Diversity of repC plasmid-replication sequences in Rhizobium leguminosarum Free

Abstract

Homologues of the plasmid replicator gene were detected and characterized in a sample of strains. Conserved PCR primers were designed from published sequences of they amplified a fragment of about 750 bp from 39 out of 41 strains tested, and also from several strains, including Restriction endonuclease digestion showed that the PCR product from individual strains, though uniform in size, was often heterogeneous in sequence. PCR products from 24 field isolates of from France, Germany and the UK were cloned and partially sequenced from both ends. Phylogenies constructed from the 5′ and 3′ ends (200 bp each) were largely congruent and demonstrated four clearly defined groups plus several unique strains. Published sequences fall within the phylogeny of sequences, though not within any of the four groups. Specific pairs of PCR primers were designed for each of the four groups; 29 out of 41 strains gave a PCR product of the expected size with more than one group-specific primer pair. We hypothesize that the sequence groups correspond to incompatibility groups of plasmids.

Loading

Article metrics loading...

/content/journal/micro/10.1099/13500872-142-7-1705
1996-07-01
2024-03-28
Loading full text...

Full text loading...

/deliver/fulltext/micro/142/7/mic-142-7-1705.html?itemId=/content/journal/micro/10.1099/13500872-142-7-1705&mimeType=html&fmt=ahah

References

  1. Beringer J.E. 1974; R factor transfer in Rhizobium leguminosarum. J Gen Microbiol 84:188–198
    [Google Scholar]
  2. Brewin N. J., Wood E. A., Johnston A. W. B., Dibb N. J., Hombrecher G. 1982; Recombinant nodulation plasmids in Rhizobium leguminosarum. J Gen Microbiol 128:1817 1827
    [Google Scholar]
  3. Couturier M., Bex F., Bergquist P. L., Maas W. K. 1988; Identification and classification of bacterial plasmids.. Microbiol Ree 52:375–395
    [Google Scholar]
  4. Géniaux E., Amarger N. 1993; Diversity and stability of plasmid transfer in isolates from a single field population of Rhizobium leguminosarum by. viciae.. FEMS Microbiol Ecol 102:251–260
    [Google Scholar]
  5. Hirsch P.R., Spokes J. D. 1994; Survival and dispersion of genetically-modified Rhizobia in the field and genetic interactions with native strains. FEMS Microbiol Ecol 15:147–159
    [Google Scholar]
  6. Hynes M.F., McGregor N.F. 1990; Two plasmids other than the nodulation plasmid are necessary for formation of nitrogenfixing nodules by Rhizobium leguminosarum.. Mol Microbiol 4:567–574
    [Google Scholar]
  7. Hynes M. F., Simon R., Pühler A. 1985; The development of plasmid-free strains of Agrobacterium tumefaciens by using incompatibility with a Rhizobium meliloti plasmid to eliminate pAtC58.. Plasmid 13:99–105
    [Google Scholar]
  8. Hynes M. F., Quandt J., O'Connell M. P., Pühler A. 1989; Direct selection for curing and deletion of Rhizobium plasmids using transposons carrying the Bacillus subtilis sac B gene. Gene 78:111–120
    [Google Scholar]
  9. Johnston A.W.B., Beringer J. E. 1975; Identification of the Rhizobium strains in pea root nodules using genetic markers. J Gen Microbiol 87:343–350
    [Google Scholar]
  10. Kobayashi N., Bailey M. J. 1994; Plasmids isolated from the sugar beet phyllosphere show little or no homology to molecular probes currently available for plasmid typing. Microbiology 140:289–296
    [Google Scholar]
  11. Laguerre G., Mazurier S., Amarger N. 1992; Plasmid profiles and restriction length polymorphism of Rhizobium leguminosarum bv. viciae in field populations. FEMS Microbiol Ecol 101:17–26
    [Google Scholar]
  12. de Lajudie P., Willems A., Pot B., Dewettinck D., Maestrojuan G., Neyra M., Collins M. D., Dreyfus B., Kersters K., Gillis M. 1994; Polyphasic taxonomy of rhizobia: emendation of the genus Sinorhizobium and description of Sinorhizobium meliloti comb, nov., Sinorhizobium saheli sp. nov., and Sinorhizobium teranga sp. nov.. Int J Syst Bacteriol 44:715–733
    [Google Scholar]
  13. Martinez E., Palacios R., Sanchez F. 1987; Nitrogen fixing nodules induced by Agrobacterium tumefaciens harboring Rhizobium phaseoli plasmids. J Bacteriol 169:2828–2834
    [Google Scholar]
  14. Mercado-Blanco J., Olivares J. 1993; Stability and transmissi- bility of the cryptic plasmids of Rhizobium meliloti GR4.. Arch Microbiol 160:477–485
    [Google Scholar]
  15. Mozo T., Cabrera E., Ruiz-Argüeso T. 1990; Isolation of the replication DNA region from a Rhizobium plasmid and examination of its potential as a replicón for Rhizobiaceae cloning vectors.. Plasmid 23:201–215
    [Google Scholar]
  16. Murillo J., Keen N. T. 1994; Two native plasmids of Pseudomonas syringae pathovar tomato strain PT23 share a large amount of repeated DNA including replication sequences. Mol Microbiol 12:941–950
    [Google Scholar]
  17. Nishiguchi R., Takanami M., Oka A. 1987; Characterisation and sequence determination of the hairy-root inducing plasmid pRiA4b. Mol Gen Genet 206:1–8
    [Google Scholar]
  18. Novick R.P. 1987; Plasmid incompatibility. Microbiol Rev 51:381–395
    [Google Scholar]
  19. Saitou N., Nei M. 1987; The neighbour-joining method: a new method for reconstructing phylogenetic trees.. Mol Biol Evo I 4:406–425
    [Google Scholar]
  20. Sambrook J., Fritsch E. F., Maniatis T. 1989 Molecular Cloning: a Eaboratory Manual, 2 nd edn. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory;
    [Google Scholar]
  21. O'Connell M.P., Hynes M. F., Puehler A. 1987; Incompatibility between a Rhizobium Sym plasmid and an Ri plasmid of Agrobacterium. Plasmid 18156–163
    [Google Scholar]
  22. Tabata S., Hookyaas P. J. J., Oka A. 1989; Sequence determination and characterisation of the replicator region in the tumor-inducing plasmid pTiB6S3. J Bacteriol 171:1665–1672
    [Google Scholar]
  23. Turner S.L., Young J. P. W. 1995; The replicator region of the Rhizobium leguminosarum cryptic plasmid pRL8JI. FEMS Microbiol Lett 133:53–58
    [Google Scholar]
  24. Velázquez E., Mateos P. F., Pedrero P., Dazzo F. B., Martfnez- Molina E. 1995; Attenuation of symbiotic effectiveness by Rhizobium meliloti SAF22 related to the presence of a cryptic plasmid. Appl Environ Microbiol 61:2033 2036
    [Google Scholar]
  25. Williams D.R., Thomas C. M. 1992; Active partitioning of bacterial plasmids. J Gen Microbiol 138:1–16
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/13500872-142-7-1705
Loading
/content/journal/micro/10.1099/13500872-142-7-1705
Loading

Data & Media loading...

Most cited Most Cited RSS feed