Analysis of heat shock gene expression in Lactococcus lactis MG1363 Free

Abstract

The induction of the heat shock response in subsp. strain MG1363 was analysed at the RNA level using a novel RNA isolation procedure to prevent degradation. Cloning of the and homologues was carried out. Northern blot analysis showed a similar induction pattern for and after transfer from 30 C to 43 C when MG1363 was grown in defined medium. The gene showed a 100-fold induction level 15 min after temperature shifting. Induction of the first two genes in the operon, and resembled the pattern observed for the above genes, although maximum induction was observed earlier for and Novel transcript sizes were detected in heat-shocked cells. The induction kinetics observed for suggested a different regulation for this gene. Experimental evidence for a pronounced transcriptional regulation being involved in the heat shock response in MG1363 is presented. A gene located downstream of the operon in strain MG1363, named was shown not to be regulated by heat shock.

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1996-07-01
2024-03-28
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References

  1. Akiyama Y., Shi rai Y., Ito K. 1994; Involvement of Fts H in protein assembly into and through the membrane. II. Dominant mutations affecting Fts H functions. J Biol Chem 269:5225–5229
    [Google Scholar]
  2. van Asseldonk M., Simons A., Visser H., De Vos W.M., Simons G. 1993; Cloning, nucleotide sequence and regulatory analysis of the Eactococcus lactis dna J gene. J Bacteriol 175:1637–1644
    [Google Scholar]
  3. Auffray Y., Gansei X., Thammavongs B., Boutibonnes P. 1992; Heat shock-induced protein synthesis in Eactococcus lactis subsp. lactis. Curr Microbiol 24:281–284
    [Google Scholar]
  4. Bukau B. 1993; Regulation of the Escherichia coli heat shock response. Mol Microbiol 9:671–680
    [Google Scholar]
  5. Burnett B. P., Horwich A. L., Low K. B. 1994; A carboxy- terminal deletion impairs the assembly of Gro EL and confers a pleiotropic phenotype in Escherichia coli K-12. J Bacteriol 176:6980–6985
    [Google Scholar]
  6. Eaton T., Shearman C., Gasson M. 1993; Cloning and sequencing analysis of the dna K gene region of Eactococcus lactis subsp. lactis. J Gen Microbiol 139:3253–3264
    [Google Scholar]
  7. Gasson M. 1983; Plasmid complements of Streptococcus lactis NCD0712 and other lactic streptococci after protoplast-induced curing. J Bacteriol 154:1–9
    [Google Scholar]
  8. Georgopoulos C., Welch W. J. 1993; Role of the major heat shock proteins as molecular chaperones.. Annu Reo Cell Biol 9:601–634
    [Google Scholar]
  9. Godon J. J., Delorme C., Ehrlich S. D., Renault P. 1992; Divergence of genomic sequences between Eactococcus lactis subsp. lactis and Eactococcus lactis subsp. crémor is. Appl Environ Microbiol 58:4045–4047
    [Google Scholar]
  10. Herman C., Ogura T., Tomoyasu T., Hiraga S., Akiyama Y., Ito K., Thomas R., D'Ari R., Bouloc P. 1993; Cell growth and X phage development controlled by the same essential Escherichia coli gene, fts H/Hfl B. Proc Natl Acad Sci USA 90:10861–10865
    [Google Scholar]
  11. Jensen P.R, 8r Hammer K. 1993; Minimal requirements for exponential growth of Eactococcus lactis. Appl Environ Microbiol 59:4363–4366
    [Google Scholar]
  12. Johansen E., Ki ben ich A. 1992; Characterization of Eeuconostoc isolates from commercial mixed strain mesophilic starter cultures.. J Dairy Sci 75:1186–1191
    [Google Scholar]
  13. Kanemori M., Mori H., Yura T. 1994; Effects of reduced levels of Gro E chaperones in protein metabolism : enhanced synthesis of heat shock proteins during steady-state growth of Escherichia coli. J Bacteriol 176:4235–4242
    [Google Scholar]
  14. Kim S.G., Batt C. A. 1993; Cloning and sequencing of the Eactococcus lactis subsp. lactis gro ESE operon. Gene 127:121–126
    [Google Scholar]
  15. Le Bourgeois P., Lautier M., van den Berghe L., Gasson M. J., Ritzenthaler P. 1995; Physical and genetic map of the Eactococcus lactis subsp. cremoris MG1363 chromosome: comparison with that of Lactococcus lactis subsp. lactis ILI403 reveals a large genome inversion. J Bacteriol 177:2840–2850
    [Google Scholar]
  16. Naberhaus F., Giebeler K., Bahl H. 1992; Molecular characterization of the dna K gene region of Clostridium acetobutylicum, including grp E, dnaj, and a new heat shock gene. J Bacteriol 174:3290–3299
    [Google Scholar]
  17. Nilsson D., Lauridsen A. A., Tomoyasu T., Ogura T. 1994; A Lactococcus lactis gene encodes a membrane protein with putative ATPase activity that is homologous to the essential Escherichia coli fts H gene. Microbiology 140:2601–2610
    [Google Scholar]
  18. Ogura T., Tomoyasu T., Yuki T., Morimura S., Beggs K. J., Donachie W. D., Mori H., Niki H., Hiraga S. 1991; Structure and function of the fts H gene.. Res Microbiol 142:279–282
    [Google Scholar]
  19. Riethdorf S., Völker U., Gerth U., Winkler A., Engelmann S., Hecker M. 1994; Cloning, nucleotide sequence and expression of the Bacillus subtilis Ion gene. J Bacteriol 176:6518–6527
    [Google Scholar]
  20. Sambrook J., Fritsch E. F., Maniatis T. 1989 Molecular Cloning: a Laboratory Manual, 2 nd edn. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory;
    [Google Scholar]
  21. Schmidt A., Schiesswohl M., Völker U., Hecker M., Schumann W. 1992; Cloning, sequencing, mapping and transcriptional analysis of the gro ESL operon from Bacillus subtilis. J Bacteriol 174:3993–3999
    [Google Scholar]
  22. Schulz A., Tzschaschel B., Schumann W. 1995; Isolation and analysis of the dna K operon of Bacillus subtilis. Mol Microbiol 15:421–429
    [Google Scholar]
  23. Segal G., Ron E. Z. 1995; The gro ESL operon of Agrobacterium tumefaciens'. evidence for heat shock-dependent mRNA cleavage. J Bacteriol 177:750–757
    [Google Scholar]
  24. Swindell S. R., Griffin H. G., Gasson M. 1994; Cloning, sequencing and comparison of three lactococcal L-lactate dehydrogenase genes. Microbiology 140:1301–1305
    [Google Scholar]
  25. Terzaghi B.E., Sandine W. E. 1975; Improved medium for lactic streptococci and their bacteriophages.. Appl Microbiol 29:807–813
    [Google Scholar]
  26. Wetzstein M., Völker U., Dedio J., Löbau S., Zuber U., Schiesswohl M., Herget C., Hecker M., Schumann W. 1992; Cloning, sequencing and molecular analysis of the dna K locus from Bacillus subtilis. J Bacteriol 174:3300–3310
    [Google Scholar]
  27. Yura T., Nagai H., Mori H. 1993; Regulation of the heat-shock response in bacteria.. Annu Ree Microbiol 47:321–350
    [Google Scholar]
  28. Zuber U., Schumann W. 1994; CIRCE, a novel heat shock element involved in regulation of heat shock operon dna K of Bacillus subtilis. J Bacteriol 176:1359–1363
    [Google Scholar]
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