@article{mbs:/content/journal/micro/10.1099/13500872-142-7-1659, author = "Yang, Maria and Luoh, Shiuh-Ming and Goddard, Audrey and Reilly, Dorothea and Henzel, William and Bass, Steve", title = "The bgIX gene located at 47.8 min on the Escherichia coll chromosome encodes a periplasmic β-glucosidase", journal= "Microbiology", year = "1996", volume = "142", number = "7", pages = "1659-1665", doi = "https://doi.org/10.1099/13500872-142-7-1659", url = "https://www.microbiologyresearch.org/content/journal/micro/10.1099/13500872-142-7-1659", publisher = "Microbiology Society", issn = "1465-2080", type = "Journal Article", keywords = "periplasm", keywords = "Escherichia coli", keywords = "BgIX family", keywords = "β-glucosidase", abstract = "A new Escherichia coli gene, bgIX, encoding a β-D-glucosidase (EC 3.2.1.21) has been characterized. The bgIX gene is located adjacent to the dld gene at 47.8 min or 2225 kb on the E. coli chromosome. The sequence of a 2.6 kb DNA fragment from this region revealed a large open reading frame encoding a protein of 765 amino acids. The BgIX protein was purified from the peripiasm, and amino-terminal sequencing suggests that the mature protein is derived from cleavage of a 20 residue signal peptide. A search of the sequence databases revealed that BgIX is a member of a large family of β-glucosidases from a variety of bacteria and fungi, and the plant Arabidopsis thaliana. It differs from the other four E. coli phospho-β-glucosidases in sequence and in its periplasmic rather than cytoplasmic location. The BgIX enzyme has a K m of 18 ± 1 mM and a V max of 3 ± 0.7 μmol min-1 for the colorimetric substrate o-nitrophenyl β-D-glucopyranoside.", }