RT Journal Article SR Electronic(1) A1 Grenier, DanielYR 1996 T1 Collagen-binding activity of Prevotella intermedia measured by a microtitre plate adherence assay JF Microbiology, VO 142 IS 6 SP 1537 OP 1541 DO https://doi.org/10.1099/13500872-142-6-1537 PB Microbiology Society, SN 1465-2080, AB The ability of Prevotella intermedia to bind type I collagen was investigated. A simple method in which bacterial cells were allowed to attach to collagen-coated microtitre plate wells was used to characterize the activity. All strains of P. intermedia tested, as well as those of the closely related species Prevotella nigrescens, showed a capacity to attach to the collagen film. Exponential-phase cultures of P. intermedia demonstrated a greater binding capacity than older cells. Attachment to the collagen film was inhibited by the presence of EDTA, type I and IV collagen, denatured collagen (gelatin), fibrinogen or fibronectin. Pretreatment of bacterial cells with heat (60 0C, 30 min) or proteinase K also inhibited the binding. The collagen-binding activity could be solubilized from the bacterial cell surface by incubation with Zwittergent 3-14, a zwitterionic detergent. The collagen-binding capacity of P. intermedia demonstrated in the present study represents a mechanism of colonization allowing these bacteria to attach to a tissue matrix., UL https://www.microbiologyresearch.org/content/journal/micro/10.1099/13500872-142-6-1537