1887

Abstract

The ability of to bind type I collagen was investigated. A simple method in which bacterial cells were allowed to attach to collagen-coated microtitre plate wells was used to characterize the activity. All strains of tested, as well as those of the closely related species , showed a capacity to attach to the collagen film. Exponential-phase cultures of demonstrated a greater binding capacity than older cells. Attachment to the collagen film was inhibited by the presence of EDTA, type I and IV collagen, denatured collagen (gelatin), fibrinogen or fibronectin. Pretreatment of bacterial cells with heat (60 C, 30 min) or proteinase K also inhibited the binding. The collagen-binding activity could be solubilized from the bacterial cell surface by incubation with Zwittergent 3-14, a zwitterionic detergent. The collagen-binding capacity of demonstrated in the present study represents a mechanism of colonization allowing these bacteria to attach to a tissue matrix.

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1996-06-01
2021-06-21
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References

  1. Devine D.A., Handley P.S. The relationship between coaggregation properties and surface structures of Bacteroides intermedius. Microb Ecol Health Dis (1989); 2:267–278
    [Google Scholar]
  2. Holt S.C., Bramanti T.E. Factors in virulence expression and their role in periodontal disease pathogenesis. Crit Rev Oral Biol Med (1991); 2:177–281
    [Google Scholar]
  3. Ivey M.H. Laboratory procedures in parasitology. In GradwohFs Clinical Laboratory Methods and Diagnosis (1980) Edited by Sonnenwirth A.C., Jarett L. St Louis: The C. V. Mosby Company; 8th edn, p 2194
    [Google Scholar]
  4. Jansen H.-J., Grenier D., Van Der Hoeven J.S. Characterization of immunoglobulin G-degrading proteases of Prevotella intermedia and Prevotella nigrescens. Oral Microbiol Immunol (1995); 10:138–145
    [Google Scholar]
  5. Kalfas S., Tigyi Z., Wikstrom M., Naidu A.S. Laminin binding to Prevotella intermedia. Oral Microbiol Immunol (1992); 7:235–239
    [Google Scholar]
  6. LabW S., Grenier D. Characterization of the human immunoglobulin G Fc-binding activity of Prevotella intermedia. Infect Immun (1995); 63:2785–2789
    [Google Scholar]
  7. Lantz M.S., Switalski L.M., Kornman K.S., HOdk M. Bacteroides intermedius binds fibrinogen. J Bacteriol (1985); 163:623–628
    [Google Scholar]
  8. Leschine S.B., Canale-Parola E. Rifampin as a selective agent for isolation of oral spirochetes. J Clin Microbiol (1980); 12:792–795
    [Google Scholar]
  9. Leung K.-P., Fukushima H., Sagawa H., , Walker, C B. & Clark W.B. Surface appendages, hemagglutination, and adherence to human epithelial cells of Bacteroides intermedius. Oral Microbiol Immunol (1989); 4:204–210
    [Google Scholar]
  10. Liu T., Gibbons R.J. Binding of strepto. occi of the ‘mutans’ group to type I collagen associated with apatitic surfaces. Oral Microbiol Immunol (1990); 5:131–136
    [Google Scholar]
  11. Liu T., Gibbons R.J., Hay D.I., Skobe Z. Binding of Actinomyces viscosus to collagen: association with the type 1 fimbrial adhesin. Oral Microbiol Immunol (1991); 6:1–5
    [Google Scholar]
  12. Naito Y., Gibbons R.J. Attachment of Bacteroidesgingivalis to collagenous substrata. J Dent Res (1988); 67:1075–1080
    [Google Scholar]
  13. Narayanan S.N., Page R. Connective tissues of the periodontium. Collagen Relat Res (1983); 3:33–64
    [Google Scholar]
  14. Shah H.N., Gharbia S.E. Biochemical and chemical studies on strains designated Prevotella intermedia and proposal of a new pigmented species, Prevotella nigrescens sp. nov.. Int J Syst Bacteriol (1992); 42:542–546
    [Google Scholar]
  15. Slots J., Listgarten M.A. Bacteroides gingivalis, Bacteroides intermedius and Actinobacillus actinomycetemcomitans in human periodontal diseases. J Clin Periodontol (1988); 15:85–93
    [Google Scholar]
  16. Sonnenwirth A.C. Stains and staining procedures. In GradwohFs Clinical Laboratory Methods and Diagnosis (1980) Edited by Sonnenwirth A.C., Jarett L. St Louis: The C. V. Mosby Company; 8th edn, p 1380–1
    [Google Scholar]
  17. Teanpaisan R., Douglas C.W.I., Walsh T.F. Characterization of black-pigmented anaerobes isolated from diseased and healthy periodontal sites. J Periodontal Res (1995); 30:245–251
    [Google Scholar]
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