1887

Abstract

A comparative chemical and serological study of the LPS of O139 and O22 was performed. Chemical analysis revealed that the sugar composition of the LPS of strain O22 was quite similar to that of O139 LPS. Each contained D-glucose, L--D--heptose, colitose (3,6-dideoxy-L-galactose), D-fructose, D-glucosamine, D-quinovosamine and D-galacturonic acid. The O-antigenic relationship between the two strains was analysed by passive haemolysis (PH) and passive haemolysis inhibition (PHI) tests with the respective LPS being used as antigens to sensitize sheep red blood cells (SRBC) and, in the latter case, as inhibitors in a PH system that consisted of LPS-sensitized SRBC, guinea-pig complement and anti-O139 or anti-O22 antiserum, both unabsorbed and absorbed with the heterologous antigen. In the PH experiment, unabsorbed anti-O139 antiserum had haemolytic titres of 66000 and 22000 against O139 LPS- and O22 LPS-sensitized SRBC, respectively; unabsorbed anti-O22 antiserum had haemolytic titres of 900 and 13000, respectively. Thus, the anti-O139 antiserum contained an antibody that reacted with a heterologous O22 antigen at a high titre (22000) and this antibody was completely removed from anti-O139 antiserum with the O22 antigen. The anti-O22 antiserum contained an antibody that reacted with the heterologous O139 antigen at a low titre (900) and this antibody was completely removed from anti-O22 antiserum with the O139 antigen. In PHI tests O139 LPS and O22 LPS each strongly inhibited (the ID of LPS ranged from 0.03 to 0.14 μg ml) the heterologous haemolytic systems of both O139 LPS-sensitized SRBC/anti-O22 antiserum and O22 LPS-sensitized SRBC/anti-O139 antiserm, which are substantially equivalent to the common antigen factor in the O139 LPS-sensitized SRBC/anti-O22 antiserum system and the common antigen factor in the O22 LPS-sensitized SRBC/anti-O139 antiserum system, respectively. The results indicated that the O antigen of O139 is closely related to that of O22 in an a,b-a,c type of relationship where a is common antigenic factor, b is an O139-specific antigenic factor and c is an O22-specific antigenic factor.

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1996-06-01
2024-03-29
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References

  1. Bitter P., Muir H.M. A modified uronic acid carbazole reaction. Anal Biochem 1962; 4:330–334
    [Google Scholar]
  2. Gerwig G.J., Kamerling J.P., Vliegenthart J.R.G. Determination of the d and l configuration of neutral monosaccharides by high-resolution capillary GLC. Carbohydr Res 1978; 62:349–357
    [Google Scholar]
  3. Hakomori S. A rapid permethylation of glycolipid and polysaccharide catalyzed by methylsulfinyl carbanion. J Biochem (Tokyo) 1964; 55:205–208
    [Google Scholar]
  4. Hisatsune K., Kondo S., Kobayashi K. Lipopoly-saccharides of non-Ol Vibrio cholerae (II): an immunochemical study on O-antigenic structure. Jpn J Med Sci Biol 1978; 31:181–184
    [Google Scholar]
  5. Hisatsune K., Kondo S., Isshiki Y., Iguchi T., Kawamata Y., Shimada T. O-Antigenic lipopolysaccharide of Vibrio cholerae 0139 Bengal, a new epidemic strain for recent cholera in the Indian subcontinent. Biochem Biophys Res Com mm 1993; 196:1309–1315
    [Google Scholar]
  6. Hitchcock P.J., Leive L., Mdkeld P.H., Rietschel E.T., Strittmatter W., Morrison D.C. Lipopolysaccharide nomenclature - past, present and future. J Bacteriol 1986; 166:699–705
    [Google Scholar]
  7. Kondo S., Haishima Y., Hisatsune K. Chemical structure of the 2-keto-3-deoxyoctonate (KDO) region of the lipopolysaccharide isolated from Ol Vibrio cholerae NIH 41R (Ogawa). Microbiol Immunol 1991a; 35:675–680
    [Google Scholar]
  8. Kondo S., Zahringer U., Seydel U., Sinnwell V., Hisatsune K., Rietschel E.T. Chemical structure of the carbohydrate backbone of Vibrio parahaemolyticus 012 lipopolysaccharide. Eur J Biochem 1991b; 200:689–698
    [Google Scholar]
  9. Kondo S., Haishima Y., Hisatsune K. Taxonomic implication of the apparent undetectability of 3-deoxy-D-wa««(5-2-octulosonate (Kdo) in lipopolysaccharides of representatives of the family Vibrionaceae and the occurrence of Kdo 4-phosphate in their inner-core regions. Carbohydr Res 1992; 231:55–64
    [Google Scholar]
  10. Luderitz O., Westphal O., Staub A.M., Nikaido H. Isolation and chemical and immunological characterization of bacterial lipopolysaccharides. In Microbial Toxins 1971 Edited by Weinbaum G., Kadis S., Ajl S.J. London: Academic Press; 4 pp 145–233
    [Google Scholar]
  11. Sakazaki R., Donovan J. Serology and epidemiology of Vibrio cholerae and Vibrio mimicus. In Methods in Microbiology 1984 Edited by Bergan T. London: Academic Press; pp 271–289
    [Google Scholar]
  12. Shimada T., Sakazaki R. R antigen of Vibrio cholerae. Jpn J Med Sci Biol 1988; 26:155–160
    [Google Scholar]
  13. Shimada T., Nair G.B., Deb B.C., Albert M.J., Sack R.B., Takeda Y. Outbreak of Vibrio cholerae non-Ol in India and Bangladesh. Eancet 1993; 341:1347
    [Google Scholar]
  14. Shimada T., Arakawa E., Itoh K., Okitsu T., Matsushima A., Asai Y., Yamai S., Nakazato T., Nair G.B., Albert M.J., Takeda Y. Expanded serotyping scheme for Vibrio cholerae. Curr Microbiol 1994a; 28:175–178
    [Google Scholar]
  15. Shimada T., Arakawa E., Itoh K., Nakazato T., Okitsu T., Yamai S., Kusum M., Nair G.B., Takeda Y. Two strains of Vibrio cholerae non-Ol possessing somatic (O) antigen factors in common with V, cholerae serogroup 0139 synonym ‘Bengal’. Curr Microbiol 1994b; 29:331–333
    [Google Scholar]
  16. Tsai C.M., Frasch C.E. A sensitive silver stain for detecting lipopolysaccharides in polyacrylamide gels. Anal Biochem 1982; 119:115–119
    [Google Scholar]
  17. Weissbach A., Hurwitz J. The formation of 2-keto-3-deoxyheptonic acid in extracts of Escherichia coli B. J Biol Chem 1959; 234:705–709
    [Google Scholar]
  18. Westphal O., Uderitz O.L., Bister R. Über die Extraktion von Bakterien mit Phenol/Wasser. Z Naturforsch 1952; 7b:148–155
    [Google Scholar]
  19. Wilkinson S.G. Composition and structure of bacterial lipopolysaccharides. In Surface Carbohydrates of the Procaryotic Cells 1977 Edited by Sutherland I.W. London: Academic Press; pp 97–175
    [Google Scholar]
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