1887

Abstract

The nucleotide sequence of a 12361 bp DNA segment in the 76 region of the chromosome has been determined. Ten putative ORFs were identified. The deduced amino acid sequences of the products of two of them ( and ) exhibited high similarity to those of (6-phospho-β-glucosidase gene) and [permease (the IIC domain) of the phosphotransferase system (PTS)], respectively, in the operon of . The C-terminal region of Glv-2 exhibited similarity to the entire region of GlvB (the IIB domain of PTS) of , suggesting fusion of the and genes in and seem to form an operon of a phosphoenolpyruvate:sugar PTS, followed by a presumed four-membered operon of an ABC transport system. Moreover, a presumed sugar symporter and its regulatory genes were located in this region.

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1996-06-01
2024-10-05
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References

  1. Akamatsu T., Sekiguchi J. Genetic mapping and properties of filamentous mutations in Bacillus subtilis. Agric Biol Chem 1987; 51:2901–2909
    [Google Scholar]
  2. Allikmets R., Gerrard B., f Court D., Dean M. Cloning and organization of the abc and mdl genes of Escherichia coli: relationship to eukaryotic multidrug resistance. Gene 1993; 136:231–236
    [Google Scholar]
  3. Anagnostopoulos C., Piggot P.J., Hoch J.A. The genetic map of Bacillus subtilis. In Bacillus subtilis and Other Grampositive Bacteria'. Biochemistry 1993 Edited by Sonenshein A.L., Hoch J.A., Losick R. Washington, DC: American Society for Microbiology; Physiology and Molecular Genetics, pp 425–461
    [Google Scholar]
  4. Chow L.M., Wong A.K., Ullman B., Wirth D.F. Cloning and functional analysis of an extrachromosomally amplified multidrug resistance-like gene in Leishmania enriettii. Mol Biochem Parasitol 1993; 60:195–208
    [Google Scholar]
  5. Cohen S.P., Hächler H., Levy S.B. Genetic and functional analysis of the multiple antibiotic resistance (mar) locus in Escherichia coli. J Bacteriol 1993; 175:1484–1492
    [Google Scholar]
  6. Davis E.O., Henderson P.J. The cloning and DNA sequence of the gene xylE for xylose-proton symport in Escherichia coli K12. J Biol Chem 1987; 262:13928–13932
    [Google Scholar]
  7. De Cr£cy-Lagard V., Bouvet O.M.M., Lejeune P., Danchin A. Fructose catabolism in Xanthomonas campestris pv campestris Sequence of the pts operon, characterization of the fructose-specific enzymes. J Biol Chem 1991; 266:18154–18161
    [Google Scholar]
  8. Fath M.J., Kolter R. ABC transporters: bacterial exporters. Microbiol Rev 1993; 57:995–1017
    [Google Scholar]
  9. Felmlee T., Pellett S., Welch R.A. Nucleotide sequence of an Escherichia coli chromosomal hemolysin. J Bacteriol 1985; 163:94–105
    [Google Scholar]
  10. Gilson L., Mahanty H.K., Kolter R. Genetic analysis of an MDR-like export system: the secretion of colicin V. EMBO J 1990; 9:3875–3894
    [Google Scholar]
  11. Itaya M., Tanaka T. Complete physical map of the Bacillus subtilis 168 chromosome constructed by a gene-directed mutagenesis method. J Mol Biol 1991; 220:631–648
    [Google Scholar]
  12. Le Coq D., Lindner C. Jr, Uger S., Steinmetz M., Ulke J. New β-glucoside (bgl) genes in Bacillus subtilis: the bglP gene product has both transport and regulatory functions similar to those of BglF, its Escherichia coli homolog. J Bacteriol 1995; 177:1527–1535
    [Google Scholar]
  13. Lei S.-P., Lin H.-C., Heffernan L., Wilcox G. araB gene and nucleotide sequence of the araC gene of Erwinia carotovora. J Bacteriol 1985; 164:717–722
    [Google Scholar]
  14. Maiden M.C.J., Davis E.O., Baldwin S.A., Moore D.C.M., Henderson P.J.F. Mammalian and bacterial sugar transport proteins are homologous. Nature 1987; 325:641–643
    [Google Scholar]
  15. Parker L.L., Hall B.G. Characterization and nucleotide sequence of the cryptic cel operon of Escherichia coli K12. Genetics 1990; 124:455–471
    [Google Scholar]
  16. Plunkett G., Burland V., Daniels D.L., Blattner F.R. Analysis of the Escherichia coli genome III DNA sequence of the region from 87-2 to 89-2 minutes. Nucleic Acids Res 1993; 21:3391–3398
    [Google Scholar]
  17. Poulsen L.K., Larsen N.W., Molin S., Andersson P. Analysis of an Escherichia coli mutant strain resistant to the cell-killing function encoded by the gef gene family. Mol Microbiol 1992; 6:895–905
    [Google Scholar]
  18. Prior T.I., Kornberg H.L. Nucleotide sequence oifruA, the gene specifying enzyme Ilfru of the phosphoenolpyruvate-dependent sugar phosphotransferase system in Escherichia coli K12. J Gen Microbiol 1988; 134:2757–2768
    [Google Scholar]
  19. Reizer J., Michotey V., Reizer A., Saier M.H. Jr Novel phosphotransferase system genes revealed by bacterial genome analysis: unique, putative fructose- and glucoside-specific systems. Protein Sci 1994; 3:440–450
    [Google Scholar]
  20. Saier M.H. Jr, Reizer J. The bacterial phosphotransferase system: new frontiers 30 years later. Mol Microbiol 1994; 13:755–764
    [Google Scholar]
  21. Sambrook J., Fritsch E.F., Maniatis T. Molecular Cloning: a Laboratory Manual 1989 Cold Spring Harbor, NY: Cold Spring Harbor Laboratory;
    [Google Scholar]
  22. Wu L.-F., Saier M.H. Jr Nucleotide sequence of the fruA gene, encoding the fructose permease of the Rhodobacter capsulatus phosphotransferase system, and analyses of the deduced protein sequence. J Bacteriol 1990; 172:7167–7178
    [Google Scholar]
  23. Yoshida K., Sano H., Seki S., Oda M., Fujimura M., Fujita Y. Cloning and sequencing of a 29 kb region of the Bacillus subtilis genome containing the hut and wap A loci. Microbiology 1995; 141:337–343
    [Google Scholar]
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