The P protein, encoded by has a central role in the control of nitrogen metabolism in nitrogen-fixing prokaryotes. The gene of was isolated and sequenced. The deduced amino acid sequence had very high sequence identity to other P proteins. The gene, encoding glutamine synthetase, was located 135 bp downstream of and was partially sequenced. is cotranscribed with from a promoter with high similarity to the s-dependent promoter consensus sequence. A putative s promoter was also identified further upstream of Northern blotting analyses showed that in addition is either transcribed from an unidentified promoter or, more likely, that the transcript is processed to give the mRNA. The total level of the two transcripts was much higher in nitrogen-fixing cells than in ammonia-grown cells.


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