1887

Abstract

Chromosomal and symbiosis-related genotypes of rhizopine-producing and non-producing isolates of and were examined by multilocus enzyme electrophoresis and RFLP. The distribution of rhizopine production in both species was found to be independent of host genotype. Conversely, rhizopine production was associated with particular symbiotic plasmid types. This association may explain the observed distribution of rhizopine production in and . Rhizopine synthesis () genes showed greater sequence divergence than rhizopine catabolism () genes in both and . Furthermore, and genes were less divergent in than , suggesting a more recent evolution in the former species.

Loading

Article metrics loading...

/content/journal/micro/10.1099/13500872-142-5-1059
1996-05-01
2021-07-31
Loading full text...

Full text loading...

/deliver/fulltext/micro/142/5/mic-142-5-1059.html?itemId=/content/journal/micro/10.1099/13500872-142-5-1059&mimeType=html&fmt=ahah

References

  1. Dooley J.H., Harrison S.P. Phylogenetic grouping and identification of Rhizobium isolates on the basis of random amplified polymorphic DNA profiles. Can J Microbiol 1993; 39:655–673
    [Google Scholar]
  2. Downie J.A., Hombrecher G., Ma Q.-S., Knight C.D., Wells B., Johnston A.W.B. Cloned nodulation genes of Rhizobium leguminosarum determine host range specificity. Mol Gen Genet 1983; 190:359–365
    [Google Scholar]
  3. Eardly B.D., Materon L.A., Smith N.H., Johnson D.A., Rumbaugh M.D., Selander R.K. Genetic structure of natural populations of the nitrogen-fixing bacterium Rbif(pbium meliloti. Appl Environ Microbiol 1990; 56:187–194
    [Google Scholar]
  4. Evans P., Howieson J. Better inoculants improve persistence and production of lucerne. Aust Grain April 1993; May:12–13
    [Google Scholar]
  5. Gordon D.M., Wexler M., Reardon T.B., Murphy P.J. The genetic structure of Rhizobium populations. Soil Biol Biochem 1995; 27:491–499
    [Google Scholar]
  6. Jensen E.S. Inoculation of pea by application of Rh'rgobium in the plant furrow. Plant Soil 1987; 97:63–70
    [Google Scholar]
  7. Laguerre G., Fernandez M.P., Edel V., Normand P., Amarger N. Genomic heterogeneity among French Rhizobium strains isolated from Phaseolus vulgaris L. Int J Sjst Bacteriol 1993a; 43:761–767
    [Google Scholar]
  8. Laguerre G., Geniaux E., Mazurier S.I., Casartelii R.R., Amarger N. Conformity and diversity among field isolates of Rhigobium leguminosarum bv viciae, bv trifolii, and bv. phaseoli revealed by DNA hybridisation using chromosome and plasmid probes. Can J Microbiol 1993b; 39:412–419
    [Google Scholar]
  9. Maynard Smith J., Smith N.H., O'Rourke M., Spratt B.G. How clonal are bacteria. Proc Natl Acad Sci USA 1993; 90:4384–4388
    [Google Scholar]
  10. Murphy P.J., Heycke N., Banfalvi Z., Tate M.E., De Bruijn F.J., Kondorosi A., Tempi J., Schell J. Genes for the catabolism and synthesis of an opine-like compound in Rhizobium meliloti are closely linked and on the Sym plasmid. Proc Natl Acad Sci USA 1987; 84:493–497
    [Google Scholar]
  11. Murphy P.J., Heycke N., Trenz S.P., Tatet P., De Bruijn F.J., Schell J. Synthesis of an opine-like compound, a rhizopine, in alfalfa nodules is symbiotically regulated. Proc Natl Acad Sci USA 1988; 85:9133–9137
    [Google Scholar]
  12. Murphy P.J., Wexler M., Grezemski W., Rao J.P., Gordon D.M. Rhizopines - their role in symbiosis and competition. Soil Biol Biochem 1995; 27:525–529
    [Google Scholar]
  13. Nei M., Li W.-H. Mathematical model for studying genetic variation in terms of restriction endonucleases. Proc Natl Acad Sci USA 1979; 76:5269–5273
    [Google Scholar]
  14. Rao J.P., Grezemski W., Murphy J.P. Rhispbium meliloti lacking mos A synthesizes the rhizopine scyllo-inosamine in place of 3-0-methyl-jry//o-inosamine. Microbiology 1995; 141:1683–1690
    [Google Scholar]
  15. Roberts G.P., Leps W.T., Sliver L.E., Brill W.J. Use of two-dimensional polyacrylamide gel electrophoresis to identify and classify Rhispbium strains. Appl Environ Microbiol 1980; 39:414–422
    [Google Scholar]
  16. Rohlf F.J. Numerical taxonomy and multivariate analysis system, version 18.0 1993 New York: Exeter Software;
    [Google Scholar]
  17. Rossbach S., Rasul G., Schneider M., Eardly B., De Bruijn F.J. Structural and functional conservation of the rhizopine catabolism (moc) locus is limited to selected Rhipobium meliloti strains and unrelated to their geographical origin. Mol Plant-Microbe Interact 1995; 4:549–559
    [Google Scholar]
  18. Saint C.P., Wexler M., Tate M., Murphy P.J. Characterization of genes for synthesis and catabolism of a new rhizopine induced in nodules by Rhizobium meliloti Rm220-3: extension of the rhizopine concept. J Bacteriol 1993; 175:5205–5215
    [Google Scholar]
  19. Sambrook J., Fritsch E.F., Maniatis T. Molecular Cloning: a Laboratory Manual 1989 2nd edn Cold Spring Harbor, NY: Cold Spring Harbor Laboratory;
    [Google Scholar]
  20. Segovia L., Pinero D., Palacios R., Martinez-Romero E. Genetic structure of a soil population of non symbiotic Rhizpbium leguminosarum. Appl Environ Microbiol 1991; 57:426–433
    [Google Scholar]
  21. Segovia L., Young J.P.W., Martinez-Romero E. Reclassification of American Rhizobium leguminosarum biovar pha-seoli type 1 strains as Rbfobium etli sp. nov. Int J Syst Bacteriol 1993; 43:372–374
    [Google Scholar]
  22. Selander R.K., Caugant D.A., Ochman H., Musser J.M., Gilmour M.N., Whittam T.S. Methods of multi locus enzyme electrophoresis for bacterial genetics and systematics. Appl Environ Microbiol 1986; 51:873–884
    [Google Scholar]
  23. Sokal R.R., Rohlf F.J. Biometry 1981 New York: W. H. Freeman; pp 787–795
    [Google Scholar]
  24. Tempe J., Petit A. La Piste des opines. In Molecular Genetics of the Bacteria Riant Interaction 1983 Edited by Piihler A. Berlin: Springer-Verlag; pp 14–32
    [Google Scholar]
  25. Truchet G., Debelle F., Vasse J., Terzaghi B., Garnerone A.-M., Rosenberg C., Batut J., Maillet F., Denarii J. Identification of a Rhizobium meliloti pSym2011 region controlling the host specificity of root hair curling and nodulation. J Bacteriol 1985; 164:1200–1210
    [Google Scholar]
  26. Upholt W.B. Estimation of DNA sequence divergence from comparison of restriction endonuclease digests. Nucleic Acids Res 1977; 4:1257–1265
    [Google Scholar]
  27. Wexler M. Molecular biology and distribution of rhizopine genes in Rhizobium species 1994 PhD thesis, University of Adelaide, Australia;
    [Google Scholar]
  28. Wexler M., Gordon D.M., Murphy P.J. The distribution of inositol rhizopine genes in Rhiizobium populations. Soil Biol Biochem 1995; 27:531–537
    [Google Scholar]
  29. Wilson K. Preparation of genomic DNA from bacteria. In Current Protocols in Molecular Biology 1991 Edited by Ausubel F.M., Breant R., Kingston R.E., Moore D.D., Seidman J.G., Smith J.A., Struhl K. Brooklyn, NY: Green Publishing Associates & John Wiley; 1 pp 241–245
    [Google Scholar]
  30. Young J.P.W. Rhizpbium population genetics: enzyme polymorphism in isolates from peas, clover, beans and lucerne grown at the same site. J Gen Microbiol 1985; 131:2399–2408
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/13500872-142-5-1059
Loading
/content/journal/micro/10.1099/13500872-142-5-1059
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error