Chromosomal and symbiosis-related genotypes of rhizopine-producing and non-producing isolates of and were examined by multilocus enzyme electrophoresis and RFLP. The distribution of rhizopine production in both species was found to be independent of host genotype. Conversely, rhizopine production was associated with particular symbiotic plasmid types. This association may explain the observed distribution of rhizopine production in and Rhizopine synthesis () genes showed greater sequence divergence than rhizopine catabolism () genes in both and Furthermore, and genes were less divergent in than suggesting a more recent evolution in the former species.


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