1887

Abstract

has two rRNA () operons designated and . Appropriate restriction fragments of genomic DNA containing sequences immediately upstream from the 16S rRNA genes were cloned. We now report the nucleotide sequence of 552 bp upstream from the 5′-end of the Box A antitermination element of the leader region of the operon. The 5′-end of this segment of DNA was found to comprise 113 codons of an ORF encoding a protein which is significantly similar to UDP--acetylglucosamine 1-carboxyvinyl-transferase (EC 2.5.1.7), which is important to cell wall synthesis. A homologous ORF is located immediately upstream from the single ( ) operons of and leprae. Primer-extension analysis of the RNA fraction of revealed four products which were related to transcription start points; the operon appears to have a single promoter whereas the operon has three (P1, P2 and P3). Analysis of RNA revealed two products corresponding to transcripts directed by promoters homologous with P1 and P3 of the of . Thus, the promoter and upstream regions of the operon of and the operon of are homologous. The presence of P2 in and its absence from is attributable to insertions/deletions of 97 bp.

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1996-03-01
2021-05-08
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References

  1. Altschul S. F., Gish W., Miller W., Myers E. W., Lipman D. F. 1990; Basic local alignment search tool. J Mol Biol 215:403–410
    [Google Scholar]
  2. Baylis H. A., Bibb M. 1988; Transcriptional analysis of the 16S rRNA gene of the rrtiD gene of Streptomyces coelicolor A3(2). Mol Microbiol 2:569–579
    [Google Scholar]
  3. Bercovier H., Kafri O., Sela S. 1986; Mycobacteria possess a surprisingly small number of ribosomal RNA genes in relation to the size of their genome. Biochem Biophys Res Commun 136:1136–1141
    [Google Scholar]
  4. Bremer H., Dennis P. P. 1987; Modulation of chemical composition and other parameters of the cell growth rate. In Escherichia coli and Salmonella typhimurium: Cellular and Molecular Biology1527–1542 Neidhardt F. C., Ingraham J. L., Brooks Low K., Magasanik B., Schaechter M., Umbarger H. E. Washington, DC: American Society for Microbiology;
    [Google Scholar]
  5. Condon C, Philips J., Fu Z.-Y., Squires C., Squires C. L. 1992; Comparison of the seven ribosomal RNA operons in Escherichia coli . EMBOJ 11:4175–4185
    [Google Scholar]
  6. Ehrt S., Hillen W. 1994; UDP-N-acetylglucosamine 1-carboxyvinyl-transferase from Acinetobacter calcoaceticus . FEMS Microbiol Lett 117:137–142
    [Google Scholar]
  7. Gonzalez-y-Merchand J. A., Estrada-Garcia I., Colston M. J., Cox R. A. 1986; A novel method for the isolation of my co-bacterial DNA. FEMS Microbiol Lett 135:71–77
    [Google Scholar]
  8. Gourse R. L, de Boer H.A., Nomura M. 1986; DNA determinants of rRNA synthesis in E. coli, growth rate dependent regulation, feedback inhibition, upstream activation, anti-termination. Cell 44:197–205
    [Google Scholar]
  9. Ji Y-e., Colston M. J., Cox R. A. 1994a; Nucleotide sequence and secondary structures of precursor 16S rRNA of slow-growing mycobacteria. Microbiology 140:123–132
    [Google Scholar]
  10. Ji Y-e., Kempsell K., Colston M. J., Cox R.A. 1994b; Nucleotide sequences of the spacer-1, spacer-2 and trailer regions of the rrn operons and secondary structures of precursor 23S rRNA and precursor 5S rRNA of slow-growing mycobacteria. Microbiology 140:1763–1773
    [Google Scholar]
  11. Ji Y-e., Colston M. J., Cox R. A. 1994c; The ribosomal RNA (rrn) operons of fast-growing mycobacteria: primary and secondary structures and their relation to rrn operons of pathogenic slow-growers. Microbiology 140:2829–2840
    [Google Scholar]
  12. Kempsell K. E., Ji Y-e., Estrada-G I. C. E., Colston M. J., Cox R. A. 1992; The nucleotide sequence of the promoter, 16S rRNA and spacer region of the ribosomal RNA operon of Mycobacterium tuberculosis and comparison with Mycobacterium leprae precursor rRNA. J Gen Microbiol 138:1717–1727
    [Google Scholar]
  13. Kieser T., Loss M. T., Dale J. W., Hopwood D. A. 1986; Cloning and expression of Mycobacterium bovis BCG DNA in “Streptomyces lividans”. J Bacteriol 168:72–80
    [Google Scholar]
  14. Liesack W., Pitulle C, Sela S., Stackebrandt E. 1990; Nucleotide sequence of the 16S rRNA from Mycobacterium leprae . Nucleic Acids Res 18:5558
    [Google Scholar]
  15. Liesack W., Sela S., Bercovier H., Pitulle C., Stackebrandt E. 1991; Complete nucleotide sequence of the Mycobacterium leprae 23S and 5S rRNA genes plus flanking regions and their potential in designing diagnostic oligonucleotide probes. FEBS Lett 281:114–118
    [Google Scholar]
  16. Ogasawara N., Moirya S., Yoshikawa H. 1983; Structure and organization of rRNA operons in the region of the replication origin of the Bacillus subtilis chromosome.. Nucleic Acids Res 11:6301–6318
    [Google Scholar]
  17. Predich M., Doukhan L, Nair G., Smith I. 1995; Characterization of RNA polymerase and two sigma-factor genes from Mycobacterium smegmatis . Mol Microbiol 15:355–366
    [Google Scholar]
  18. Rysavy F. R., Bishop M. J., Gibbs G. P., Williams G. W. 1992; U. K. HGMP on line computer service. Comput Appl Biosci 8:149–154
    [Google Scholar]
  19. Saiki R. K., Gelfand D. H., Stoffel S., Sharf S. J., Higuchi K., Horn G. T., Mullis K. B., Erlich H. A. 1988; Primer-directed enzymatic amplification of DNA with a thermostable DNA polymerase. Science 239:487–491
    [Google Scholar]
  20. Sela S., Clark-Curtiss J. E. 1991; Cloning and characterization of the Mycobacterium leprae putative ribosomal RNA promoter in Escherichia coli . Gene 98:123–127
    [Google Scholar]
  21. Strohl W. R. 1992; Compilation and analysis of DNA sequences associated with apparent streptomycete promoters. Nucleic Acids Res 20:961–974
    [Google Scholar]
  22. Suzuki Y., Nagata A., Ono Y., Yamada I. 1988; Complete nucleotide sequence of the 16S rRNA gene of Mycobacterium bovis BCG. J Bacteriol 470:1631–1636
    [Google Scholar]
  23. Verma A., Kringer A. K., Tyagi J. S. 1994; Functional analysis of transcription of the Mycobacterium tuberculosis 16S rDNA-encoding gene. Gene 148:113–118
    [Google Scholar]
  24. Vogelstein B., Gillespie D. 1979; Preparative and analytical purification of DNA from agarose. Proc Natl Acad Sci USA 76615–619
    [Google Scholar]
  25. van Wezel G. P., Krab I. M., Douthwaite S., Bibb M. J., Vijgenboom E., Bosch L. 1994; Transcription analysis of the Streptomyces coelicolor A3(2) rrnA operon. Microbiology 140:3357–3365
    [Google Scholar]
  26. Winder F. G., Rooney S. A. 1970; Effects of nitrogenous components of the medium on the carbohydrate and nucleic acid content oiMycobacterium tuberculosis BCG. J Gen Microbiol 63:29–39
    [Google Scholar]
  27. Zengel M., Lindahl L. 1990; Mapping of two promoters for elongation factor Tu within the structural gene for elongation factor G. Biochem Biophys Ada 1050:317–322
    [Google Scholar]
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