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Volume 142, Issue 3

Cloning, sequencing and disruption of a bromoperoxidase-catalase gene in : evidence that it is not required for chlorination in chloramphenicol biosynthesis Free

Abstract

Genomic DNA libraries of ISP5230 and of a mutant blocked at the chlorination step of chloramphenicol biosynthesis were probed by hybridization with a synthetic oligonucleotide corresponding to the N-terminal amino acid sequence of a bromoperoxidase-catalase purified from the wild-type strain. Hybridizing fragments obtained from the two strains were cloned and sequenced. Analysis of the nucleotide sequences demonstrated that the fragments contained the same 1449 bp open reading frame with no differences in nucleotide sequence. The deduced polypeptide encoded 483 amino acids with a calculated of 54200; the N-terminal sequence was identical to that of the bromoperoxidase-catalase purified from wild-type Comparison of the amino acid sequence predicted for the cloned bromoperoxidase-catalase gene () with database protein sequences showed a significant similarity to a group of prokaryotic and eukaryotic catalases, but none to other peroxidases or haloperoxidases. Replacement of the gene in the wild-type strain of with a copy disrupted by insertion of a DNA fragment encoding apramycin resistance did not prevent chloramphenicol production. The results suggest that the role of the enzyme in is related to its activity as a catalase rather than as a halogenating agent.

  • Received:
  • Accepted:
  • Revised:
  • Published Online:
Keyword(s): bromoperoxidase-catalase , chloramphenicol , gene disruption and Streptomyces venezuelae
© Society for General Microbiology 1996
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1996-03-01
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Cloning, sequencing and disruption of a bromoperoxidase-catalase gene in Streptomyces venezuelae: evidence that it is not required for chlorination in chloramphenicol biosynthesis
Microbiology 142, 657 (1996); https://doi.org/10.1099/13500872-142-3-657
/content/journal/micro/10.1099/13500872-142-3-657
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