The gene, which is disrupted by Tn5 in the acid-sensitive mutant of TG2-6, was cloned and sequenced. It encodes a protein of 541 amino acids with a calculated molecular mass of 57963 Da and an estimated pI of 9.0. The ActA protein sequence has 30% identity, and much higher similarity (69%), with the CutE protein of Like the mutant of TG2-6 is sensitive to copper. The reconstructed wild-type gene complemented the low pH- and copper-sensitive phenotype of TG2-6. Studies with an gene fusion showed that is constitutively expressed at pH 5.8 and 7.0. The gene appears to be chromosomal and is present in all seven strains of tested.


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