1887

Abstract

A 40 kb genomic clone and 2·3 kb RI subclone that rescued the DNA repair and recombination defects of the mutant were isolated and the subclone sequenced. The subclone hybridized to a cosmid in a chromosome-specific library confirming the assignment of to linkage group IV and indicating its closeness to Amplification by PCR clarified the relative positions of and A region identified within the subclone, encoding a C3HC4 zinc finger motif, was used as a probe to retrieve a cDNA clone. Sequencing of this clone showed that the gene has an ORF of 1329 bp with two introns of 51 bp and 60 bp. Expression of appears to be extremely low. The putative NUVA polypeptide has two zinc finger motifs, a molecular mass of 48906 Da and has 39% identity with the and 25% identity with the translation products. Although mutations in and produce similar phenotypes, only the mutation affects meiotic recombination. A role for in both DNA repair and genetic recombination is proposed.

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1996-03-01
2021-10-25
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References

  1. Aramayo R., Timberlake W. F. 1990; Sequence and molecular structure of the Aspergillus nidulans yA (laccase-I) gene. Nucleic Acids Res 18:3415
    [Google Scholar]
  2. Bailly V., Lamb J., Sung P., Prakash S., Prakash L. 1994; Specific complex formation between RAD6 and RAD18 proteins: a potential mechanism for targeting RAD6 ubiquitin-conjugating activity to DNA damage sites. Genes & Dev 8:811–820
    [Google Scholar]
  3. Bainbridge B. W. 1971; Macromolecular composition and nuclear division during spore germination in Aspergillus nidulans . J Gen Microbiol 66:319–325
    [Google Scholar]
  4. Ballance D. J., Turner G. 1985; Development of a high frequency of transforming vector for Aspergillus nidulans . Gene 33:321–327
    [Google Scholar]
  5. Berg J. M. 1990; Zinc fingers and other metal-binding domains. J Biol Chem 265:6513–6516
    [Google Scholar]
  6. Bergan G. G., Morris N. R. 1983; Kinetics of the nuclear division cycle of Aspergillus nidulans . J Bacteriol 156:155–160
    [Google Scholar]
  7. Brody H., Griffith J., Cuticchia A. J., Arnold J., Timberlake W. 1991; Chromosome-specific recombinant DNA libraries from the fungus Aspergillus nidulans . Nucleic Acids Res 19:3105–3109
    [Google Scholar]
  8. Bullock W. O., Fernandez J. M., Short J. M. 1987; XL1-blue: a high efficiency plasmid transforming recA Escherichia coli strain with beta-galactosidase selection. Bio/Techniques 5:376
    [Google Scholar]
  9. Cassier-Chauvat C., Fabre F. 1991; A similar defect in UV-induced mutagenesis conferred by the rad6 and rad18 mutations of Saccharomyces cerevisiae . Mutat Res 254:247–253
    [Google Scholar]
  10. Clutterbuck A. J. 1994; Linkage map and loci. Aspergillus: 50 Years On. Prog Ind Microbiol 29:791–827
    [Google Scholar]
  11. Cove D. J. 1966; The induction and repression of nitrate reductase in the fungus Aspergillus nidulans . Biochim Biophys Acta 113:51–56
    [Google Scholar]
  12. Feinberg A. P., Vogelstein B. 1983; A technique for radiolabelling DNA restriction endonuclease fragments to high specific activity. Anal Biochem 132:6–13
    [Google Scholar]
  13. Fidel S., Doonan J. H., Morris N. R. 1988; Aspergillus nidulans contains a single actin gene which has unique intron locations and encodes a γ-actin gene. Gene 70:283–293
    [Google Scholar]
  14. Game J. C. 1983; Radiation sensitive mutants and DNA repair in yeast. Yeast Genetics : Fundamental and Applied Aspects109–137 Spencer J. F. T., Spencer D. M., Smith A. R. W. New York: Springer-Verlag;
    [Google Scholar]
  15. Genetics Computer Group 1994 Program Manual for the Wisconsin Package, Version 8 Madison, Wisconsin, USA: GCG;
    [Google Scholar]
  16. Gribskov M., Burgess R. R. 1986; Sigma factors from Escherichia coli B subtilis phage SP-01 and phage T4 are homologous proteins. Nucleic Acids Res 14:6745–6763
    [Google Scholar]
  17. Hanahan D. 1983; Studies on transformation of Escherichia coli with plasmids. J Mol Biol 166:557–580
    [Google Scholar]
  18. Haynes R. H., Kunz B. I. 1981; Repair and mutagenesis in yeast. The Molecular Biology of the Yeast Saccharomyces. Life Cycle and Inheritance371–414 Strathern J. N., Jones E. W., Broach J. R. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory;
    [Google Scholar]
  19. Ishii C., Nakamura K., Inoue H. 1991; A novel phenotype of an excision-repair mutant in Neurospora crassa: mutagen sensitivity of mus-18 mutant is specific to UV. Mol & Gen Genet 228:33–39
    [Google Scholar]
  20. Jones J. S., Prakash L. 1990; Transcript levels of Saccharomyces cerevisiae DNA repair gene RAD18 increase in UV irradiated cells and during meiosis but not during the mitotic cell cycle. Nucleic Acids Res 19:893–898
    [Google Scholar]
  21. Jones J. S., Weber S., Prakash L. 1988; The Saccharomyces cerevisiae RAD18 gene encodes a protein that contains potential zinc finger domains for nucleic acid binding and a putative nucleotide binding sequence. Nucleic Acids Res 16:7119–7131
    [Google Scholar]
  22. Käfer E., Chae S. 1994; Phenotypic and epistatic grouping of hypo- and hyper-rec mus mutants in Aspergillus . Curr Genet 25:223–232
    [Google Scholar]
  23. Käfer E., Mayor O. 1986; Genetic analysis of DNA repair in Aspergillus: evidence for different types of MMS-sensitive hyperrec mutants. Mutat Res 161:119–134
    [Google Scholar]
  24. Osman F., Cotton C., Tomsett B., Strike P. 1991; Isolation and characterisation of nuv11, a mutation affecting meiotic and mitotic recombination in Aspergillus nidulans . Biochimie 73:321–327
    [Google Scholar]
  25. Pontecorvo G., Roper J. A., Hemmons L. M., MacDonald K. D., Button A. W. J. 1953; The genetics of Aspergillus nidulans . Adv Genet 5:141–238
    [Google Scholar]
  26. Prakash S., Sung P., Prakash L. 1993; DNA repair genes and proteins of Saccharomyces cerevisiae . Annu Rev Genet 27:33–70
    [Google Scholar]
  27. Raeder U., Broda P. 1985; Rapid preparation of DNA from filamentous fungi. Letts Appl Microbiol 1:17–20
    [Google Scholar]
  28. Roman H., Ruzinski M. M. 1990; Mechanisms of gene conversion in Saccharomyces cerevisiae . Genetics 124:7–25
    [Google Scholar]
  29. Sambrook J., Fritsch E. F., Maniatis T. 1989 Molecular Cloning: A Laboratory Manual, Second. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory;
    [Google Scholar]
  30. Sanger F., Nicklen S., Coulson A. R. 1977; DNA sequencing with chain terminating inhibitors. Proc Natl Acad SciUSA 745463–5467
    [Google Scholar]
  31. Shatz D. G., Oettinger M. A., Baltimore D. 1989; The V(D)J recombination activating gene RAG-1 . Cell 59:1035–1048
    [Google Scholar]
  32. Sylvers L. A., Beresten S. 1993; Loss of resolution in gel electrophoresis of RNA : a problem associated with the presence of formaldehyde gradients. BioTechniques 14:380–381
    [Google Scholar]
  33. Tomita H., Soshi T., Inoue H. 1993; The Neurospora uvs-2 gene encodes a protein which has homology to yeast RAD18, with unique zinc finger motifs. Mol & Gen Genet 238:225–233
    [Google Scholar]
  34. Tomsett A. B., Cove D. J. 1979; Deletion mapping of the niiAniaD region in Aspergillus nidulans . Genet Res 34:19–32
    [Google Scholar]
  35. Wang Y. H., Prade R. A., Griffith J., Timberlake W. E., Arnold J. 1994; A fast random cost algorithm for physical mapping. Proc Natl Acad SciUSA 9111094–11098
    [Google Scholar]
  36. Waring R. B., May G. S., Morris N. R. 1989; Characterization of an inducible expression system in Aspergillus nidulans using alcA and tubulin-coding genes. Gene 79:119–130
    [Google Scholar]
  37. Weis J. H. 1994; ‘RACE no more’; an alternative approach to cloning the 5′ end of transcripts. Nucleic Acids Res 22:3427–3428
    [Google Scholar]
  38. Worthy T. E., Epler J. L. 1973; Biochemical basis of radiation sensitivity in mutants of Neurospora crassa . Mutat Res 19:167–173
    [Google Scholar]
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