Summary: A 2.7 kb HI fragment of the daunorubicin biosynthetic cluster in ATCC 29050 was shown to contain two ORFs, and whose deduced products exhibit a high sequence similarity to a number of glucose-1-phosphate thymidylyl transferases and TDP-D-glucose dehydratases, respectively. Although these genes were believed to be necessary for the synthesis of the deoxyaminosugar, daunosamine, a constituent of daunorubicin, the gene contains a frameshift in the DNA sequence that causes the premature termination of translation. A gene encoding another TDP-glucose 4,6-dehydratase, previously isolated from was identified by PCR amplification of genomic DNA. The presence of this gene explains why a mutation did not block daunorubicin production.


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