Summary: We explored different methods of introducing DNA into ‘Streptomyces toyocaensis’ and Streptomyces virginiae to construct stable recombinant strains. Plasmid pIJ702 isolated from transformed protoplasts of at a frequency of 7 × 10 transformants (μgDNA)-1. pIJ702 prepared from transformed protoplasts at a frequency of 1.5 × 10 (μgDNA)-1. suggesting that expresses restriction and modification. Plasmid pRHB126 was transduced by bacteriophage FP43 into at a frequency of 1.2 × 10 (p.f.u.). Plasmids pOJ436 and pRHB304 were introduced into by conjugation from S17-1 at frequencies of about 2 × 10 and 1 × 10 per recipient, respectively. Analysis of several exconjugants indicated that pOJ436 and pRHB304 inserted into a unique øC31 site and that some of the insertions had minimal deleterious effects on glycopeptide A47934 production. The results indicate that is a suitable host for gene cloning, whereas does not appear to be.


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