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Volume 142, Issue 12

An antigenic water-soluble glucogalactomannan extracted from cell walls of Paecilomyces fumosoroseus and Paecilomyces farinosus Free

Abstract

The water-soluble fraction (F1S) obtained after solubilizing in alkali the cell walls of four strains of and two of amounted to 8·3–14·5% of the dry cell wall material. Two polysaccharides, F1S-A (13–20%) and F1S-B (57–68%) were separated from F1S by gel permeation through Sepharose CL-6B. H and C NMR spectra of F1S-B were recorded and showed analogous structural features in the six isolates of the two species. The fractions isolated from strain CBS 375.70 were subjected to structural analysis and shown to be a (1→4)--glucan (F1S-A) and a branched (1→6)-mannan with terminal residues of β-galactopyranose (F1S-B). Polyclonal antibodies against the latter polysaccharide were obtained (titre 1/8000). These antibodies reacted specifically with the F1S-B polysaccharides obtained from the four strains of and the two strains of but they did not react with similar fractions from other species of the same or related genera. The antibodies specifically stained hyphae in indirect immunofluorescence tests.

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Keyword(s): ELISA , immunofluorescence , Paecilomyces spp., cell wall glucogalactomannans and polyclonal antibodies
© Society for General Microbiology 1996
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1996-12-01
2024-04-19
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References

  1. Adams G. A. 1965; Complete acid hydrolysis. Methods Carbohydr Chem 5:269–276
     [Google Scholar]
  2. Aoki J., Yanase K. 1970a; Studies on the nutrition and metabolism of pathogenic fungi of muscardine. 2. The influences of amino acids for the formation and multiplication of hyphal bodies of Spicaria fumoso-rosea (Wizc) Vassil and of Beauvearia bassiana(Bals.) Vuil. J Seric Sci Jpn 39:285–292
     [Google Scholar]
  3. Aoki J., Yanase K. 1970b; Phenol oxidase activity in the integument of the silk-worm Bombyx mori infected with Beauvearia bassiana and Spicaria fumoso-rosea. . J Invertebr Pathol 16:459–464
     [Google Scholar]
  4. Bartnicki-Garcia S. 1968; Cell wall chemistry, morphogenesis and taxonomy of fungi. Annu Rev Microbiol 2:87–108
     [Google Scholar]
  5. Byrd R. P. Jr Roy T. M., Fields C. L., Lynch J. A. 1992; Paecilomyces variotii pneumonia in a patient with diabetes mellitus. J Diabetes Complications 6:150–153
     [Google Scholar]
  6. Ciucanu I., Kerek F. 1984; A simple and rapid method for the permethylation of carbohydrates. Carbohydr Res 131:209–217
     [Google Scholar]
  7. De Repentigny L. 1989; Serological tecniques for diagnosis of fungal infections. Eur J Clin Microbiol Infect Dis 8:362–375
     [Google Scholar]
  8. De Repentigny L., Boushira M., Ste-Marie L., Bosisio G. 1987; Detection of galactomannan antigenemia by enzyme immunoassay in experimental invasive aspergillosis. J Clin Microbiol 25:863–867
     [Google Scholar]
  9. De Ruiter G. A., Smid P., Van der Lugt A. W., Van Boom J. H., Notermans S. H. W., Rombouts F. M. 1991; Immunogenic extracellular polysaccharides of Mucorales. . In Fungal Cell Wall and Immune Response NATO ASI series H53 pp. 169–180 Latgé J. P., Boucias D. Edited by Berlin & Heidelberg: Springer;
     [Google Scholar]
  10. De Ruiter G. A., Notermans S. H. W., Rombouts F. M. 1993; New methods in food mycology. Trends Food Sci Technol 4:91–97
     [Google Scholar]
  11. Dewey F. M., MacDonald M. M., Phillips S. I., Priestley R. A. 1990; Development of monoclonal-antibody-ELISA and DIP-STICK immunoassays for Penicillium islandicum in rice grains. J Gen Microbiol 136:753–760
     [Google Scholar]
  12. Domenech J., Prieto A., Bernabé M., Leal J. A. 1994; Cell wall polysaccharides of four strains of P. variotii. . Curr Microbiol 28:169–173
     [Google Scholar]
  13. Douglas King A. J. R., Michener H. D., Ito K. A. 1969; Control of Byssochlamys and related heat-resistant fungi in grape products. Appl Microbiol 18:166–173
     [Google Scholar]
  14. Dubois M., Gilles K. A., Rebers P. A. 1956; Colorimetric method for determination of sugars and related substances. Anal Chem 28:350–356
     [Google Scholar]
  15. Engel G., Teuber M. 1991; Heat resistence of ascospores of Byssochlamys nivea in milk and cream. Int J Food Microbiol 12:225–233
     [Google Scholar]
  16. Gómez-Miranda B., Rupérez P., Leal J. A. 1981; Changes in chemical composition during germination of Botrjtis cinerea scle-rotia. Curr Microbiol 6:243–246
     [Google Scholar]
  17. Gómez-Miranda B., Prieto A., Leal J. A. 1990; Chemical composition and characterization of a galactomannoglucan from Gliocladium viride. . FEMS Microbiol Lett 70:331–336
     [Google Scholar]
  18. Jiménez-Barbero J., Prieto A., Gómez-Miranda B., Leal J. A., Bernabé M. 1995; Chemical structure of fungal cell-wall poly�saccharides isolated from Microsporum gypseum and related species of Microsporum and Trychophjton. . Carbohydr Res 272:121–128
     [Google Scholar]
  19. Kamphuis H. J., De Ruiter G. A., Veeneman G. H., Van Boom J. H., Rombouts F. M., Notermans S. H. W. 1992; Detection of Aspergillus and Penicillium extracellular polysaccharides (EPS) by ELISA: using antibodies raised against acid hydrolysed EPS. Antonie Leeuwenhoek 61:323–332
     [Google Scholar]
  20. Kaufman L., Turner L. F., McLaughlin D. W. 1989; Indirectenzyme-linked immunosorbent assay for zygomycosis. J Clin Microbiol 27:1979–1982
     [Google Scholar]
  21. Laine R. A., Esselman W. J., Sweeley C. C. 1972; Gas-liquid chromatography of carbohydrates. Methods Enzymol 28:159–167
     [Google Scholar]
  22. Leal J. A., Rupérez P., Gómez-Miranda B. 1979; Extracellular glucan production by Botrytis cinerea. . TransBr MycolSoc 72:172–176
     [Google Scholar]
  23. Leal J. A., Gómez-Miranda B., Bernabè M., Cano J., Guarro J. 1992a; The chemical composition of six species of Aphanoascus: the taxonomic significance of the presence of ±-(l-2)-(l-6) mannan and α-(l-4) glucan. Mycol Res 96:363–368
     [Google Scholar]
  24. Leal J. A., Guerrero C., Gómez-Miranda B., Prieto A., Bernabé M. 1992b; Chemical and structural similarities in wall polysaccharides of some Penicillium, Eupenicillium and Aspergillus. . FEMS Microbiol Lett 90:165–168
     [Google Scholar]
  25. Leal J. A., Gómez-Miranda B., Prieto A., Bernabé M. 1993; Differences in cell wall polysaccharides of several species of Eupenicillium. . FEMS Microbiol Lett 108:341–346
     [Google Scholar]
  26. Leal J. A., Jiménez-Barbero J., Gómez-Miranda B., Parra E., Prieto A., Bernabé M. 1995; Structural investigation of cell- wall polysaccharides from Neosartorya: relationships with their putative anamorphs of Aspergillus. . Carbohydr Res 273:255–262
     [Google Scholar]
  27. Marbanmendoza N., Garciae R., Dicklow M. B., Zuckerman B. M. 1992; Studies on Paecilomyces marquandii from nematode supressive Chinampa Soils. J Chem Ecol 18:775–783
     [Google Scholar]
  28. Naidu J., Singh S. M. 1992; Hyalohyphomycosis caused by Paecilomyces variotii: a case report, animal pathogenity and ‘in vitro’ sensitivity. Antonie Leeuwenhoek 62:225–230
     [Google Scholar]
  29. Notermans S., Heuvelman C. J. 1985; Immunological detection of moulds in food by using the enzyme-linked immunosorbent assay (ELISA); preparation of antigens. Int J Food Microbiol 2:247–258
     [Google Scholar]
  30. Notermans S., Dufrenne J., Wijnands L. M., Engel H. W. B. 1988; Human serum antibodies to extracellular polysaccharides (EPS) of moulds. J Med Vet Mycol 26:41–48
     [Google Scholar]
  31. Parra E., Jiménez-Barbero J., Bernabé M., Leal J. A., Prieto A., Gómez-Miranda B. 1993; Structural investigation of two cell- wall polysaccharides of Penicillium expansum strains. Carbohydr Res 167:269–278
     [Google Scholar]
  32. Parra E., Jiménez-Barbero J., Bernabé M., Leal J. A., Prieto A., Gómez-Miranda B. 1994; Structural studies of fungal cell-wall polysaccharides from two strains of Talaromycesflavus. . Carbohydr Res 251:315–325
     [Google Scholar]
  33. Pendland J. C. 1993; Variations in the ability of galactose and mannose-specific lectins to bind to cell wall surfaces during growth of the insect pathogenic fungus Paecilomyces farinosus. . Eur J Cell Biol 60:322–330
     [Google Scholar]
  34. Prieto A., Bernabé M., Leal J. A. 1995; Isolation, purification and chemical characterization of alkali-extractable polysaccharides from the cell walls of Talaromyces species. Mycol Res 99:69–75
     [Google Scholar]
  35. Rupérez P., Leal J. A. 1987; Mannoglucogalactans from the cell walls of Penicillium erythromellis: isolation and partial characterization. Carbohydr Res 167:269–278
     [Google Scholar]
  36. Samson R. A. 1974; Paecilomyces and some allied Hyphomycetes. Stud Mycol 6:1–119
     [Google Scholar]
  37. Sugiyama J., Fukagawa M., Chiu S. W., Fomagata K. 1985; Cellular carbohydrate composition, DNA base composition, ubiquinone systems and diazonium blue B color test in the genera Rhodosporidium, Leucosporidium, Rhodotorula and related basidio-mycetous yeast. J Gen Appl Microbiol 31:519–550
     [Google Scholar]
  38. Weijman A. C. M., Golubev W. I. 1987; Carbohydrate patterns and taxonomy of yeasts and yeast-like fungi. In The Expanding Realm of Yeast-like Fungi pp. 361–371 de Hoog G. S., Smith M. T. H., Weijman A. C. M. Edited by Amsterdam: Elsevier Science.;
     [Google Scholar]
  39. Weijman A. C. M., Van der Walt J. P. 1989; Carbohydrate profiles and the taxonomic concept in the Lipomycetaceae. . Stud Mycol 31:193–199
     [Google Scholar]
  40. Weijman A. C. M., Vlug I. J. A., Van Eijk G. W. 1982; Carbohydrate patterns. Stud Mycol 22:50–53
     [Google Scholar]
  41. Wessels J. G. H., Kreger D. R., Marchant R., Regensburg B. A., De Vries O. M. H. 1972; Chemical and morphological characterization of the hyphal wall surface of the Basidiomycete Schizphylum commune. . Biochim Biophys Acta 273:346–358
     [Google Scholar]
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An antigenic water-soluble glucogalactomannan extracted from cell walls of Paecilomyces fumosoroseus and Paecilomyces farinosus
Microbiology 142, 3497 (1996); https://doi.org/10.1099/13500872-142-12-3497
/content/journal/micro/10.1099/13500872-142-12-3497
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