1887

Abstract

Summary: In a phosphoenolpyruvate: mannose phosphotransferase system (mannose-PTS) has been characterized and it was shown to be involved in glucose and mannose transport, but no glucose-specific PTS activity could be detected. A 2.1 kb DNA fragment amplified by PCR from the genome was sequenced. Sequence analysis showed four ORFs which could encode proteins similar to PTS transporters EIIA, EIIB, EIIC and EIID of the mannose class. The expression of the gene (encoding EIIB) from in a mutant of impaired in EII activity restored this activity. Furthermore, this DNA fragment complemented the regulatory function of LevE (EIIB) in a -deficient mutant, suggesting that the protein encoded by could also play a regulatory role in

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1996-12-01
2021-07-28
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