1887

Abstract

Summary: Internal solutes in a halotolerant sp. (strain JCM 6894) were analysed by H NMR spectroscopy. At the lower osmolarity of complex medium, the main compatible solutes in the cells were glutamate, glycine betaine and hydroxyproline. At higher external salt concentrations, ectoine (1,4,5,6-tetrahydro-2-methyl-4-pyrimidinecarboxylic acid) levels increased and this became the predominant compatible solute at >1.5 M NaCl or KCl. When sp. was grown in the absence of yeast extract, glycine betaine levels were reduced to about half and a new solute, glycine, was detected. Irrespective of whether the medium contained yeast extract, sp. accumulated hydroxyproline, which has not been previously reported as an osmolyte in halophilic eubacteria. Trehalose and glutamate were the main solutes in cells grown in chemically defined medium containing 0–0·5 M NaCl. When grown in the presence of > 1·5 M NaCl, both ectoine and hydroxyectoine became dominant, as was also the case in complex medium. Additional compatible solutes detected were γ-aminobutyrate and proline, neither of which were detected in cells grown in complex medium. Regardless of the medium composition and incubation time, ectoine levels were high at high osmolarity, indicating that the halotolerant nature of sp. is closely related to its accumulation of ectoine. From its accumulation of compatible solutes, we suggest that sp. JCM 6894 is a different species from other members of the genus

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1996-12-01
2024-04-19
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References

  1. Amezaga M. -R., Davidson I., McLaggan D., Verheul A., Abee T., Booth I. R. 1995; The role of peptide metabolism in the growth ofListeria monocytogenesATCC 23074 at high osmolarity. Microbiology 141:41–49
    [Google Scholar]
  2. Bernard T., Jebbar M., Rassouli Y., Himdi-Kabbab S., Hamelin J., Blanco C. 1993; Ectoine accumulation and osmotic regulation inBrevibacterium linens. J Gen Microbiol 139:129–136
    [Google Scholar]
  3. Brown A. D. 1976; Microbial water stress. Bacterial Rev 40:803–846
    [Google Scholar]
  4. Cayley S., Record M. T.JR Lewis B. A. 1989; Accumulation of 3-(IV-morpholino)propanesulfonate by osmotically stressedEscherichia coliK-12. J Bacteriol 171:3597–3602
    [Google Scholar]
  5. Csonka L. N. 1989; Physiological and genetic responses of bacteria to osmotic stress. Microbiol Rev 33:121–147
    [Google Scholar]
  6. Dinnbier U., Limpinsel E., Schmid R., Bakker E. P. 1988; Transient accumulation of potassium glutamate and its replacement by trehalose during adaptation of growing cells ofEscherichia coliK- 12 to elevated sodium chloride concentrations. Arch Microbiol 150:348–357
    [Google Scholar]
  7. Frings E., Kunte H. J., Galinski E. A. 1993; Compatible solutes in representatives of the generaBrevibacteriumandCorynebacterium: occurrence of tetrahydropyrimidines and glutamine. FEMS Microbiol Lett 109:25–32
    [Google Scholar]
  8. Galinski E. A. 1993; Compatible solutes of halophilic eubacteria: molecular principles, water-solute interaction, stress protection. Experientia 49:487–496
    [Google Scholar]
  9. Galinski E. A. 1995; Osmoadaptation in bacteria. Adv Microbiol Physiol 37:273–328
    [Google Scholar]
  10. Gilboa H., Kogut M., Chalamish S., Regev R., Avi-dor Y., Russell N. J. 1991; Use of23Na nuclear magnetic resonance spectroscopy to determine the true intracellular concentration of free sodium in a halophilic eubacterium. J Bacteriol 173:7021–7023
    [Google Scholar]
  11. Inbar L., Frolow F., Lapidot A. 1993; The conformation of new tetrahydro-pyrimidine derivatives in solution and in crystal. Eur J Biochem 214:897–906
    [Google Scholar]
  12. Lai M. -C., Sowers K. R., Robertson D. E., Poberts M. F., Gunsalus R. P. 1991; Distribution of compatible solutes in the halophilic methanogenic archaebacteria. J Bacteriol 173:5352–5358
    [Google Scholar]
  13. McLaggan D., Naprstek J., Buurman E. T., Epstein W. 1994; Interdependence of K+and glutamate accumulation during osmotic adaptation ofEscherichia coli. J Biol Chem 269:1911–1917
    [Google Scholar]
  14. Mimura H., Nagata S., Matsumoto T. 1994; Concentrations and compositions of internal free amino acids in a halotolerantBrevibacteriumsp. in response to salt stress. Biosci Biotechnol Biochem 58:1873–1874
    [Google Scholar]
  15. Nagata S. 1988; Influence of salts and pH on the growth as well as NADH oxidase of the halotolerant bacterium A505. Arch Microbiol 150:302–308
    [Google Scholar]
  16. Nagata S., Ogawa Y., Mimura H. 1991; Internal cation concentrations of the halotolerant bacteriumBrevibacteriumsp. in response to the concentrations and species of external salts. J Gen Appl Microbiol 37:403–414
    [Google Scholar]
  17. Nagata S., Adachi K., Shirai K., Sano H. 1995; 23Na NMR spectroscopy of free Na+in the halotolerant bacteriumBrevibacteriumsp. andEscherichia coli. Microbiology 141:729–736
    [Google Scholar]
  18. Oren A., Gurevich P. 1993; The fatty acid synthetase complex ofHaloanaerobium praevalensis not inhibited by salt. FEMS Microbiol Lett 108:287–290
    [Google Scholar]
  19. Pocard J. A., Smith L. T., Smith G. M. 1994; A prominent role for glucosylglycerol in the adaptation ofPseudomonas mendocinaSKB70 to osmotic stress. J Bacteriol 176:6877–6884
    [Google Scholar]
  20. Severin J., Wohlfarth A. 1992; The predominant role of recently discovered tetrahydropyrimidines for the osmoadaptation of halophilic eubacteria. J Gen Microbiol 138:1629–1638
    [Google Scholar]
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