The nucleotide sequence of the biotin () operon of wild-type Sr41 was determined. Five ORFs were identified to encode BioA (7,8-diaminopelargonic acid aminotransferase), BioB (biotin synthase), BioF (7-keto-8-aminopelargonic acid synthase), BioC (an enzyme catalysing the synthesis of pimeloyl-CoA) and BioD (dethiobiotin synthase), in this order. The operon was deduced to be transcribed divergently to the left into and to the right into the genes. The promoters and a common predicted operator for both and genes were located between the and genes. The predicted amino acid sequences of these enzymes were similar to the sequences of the corresponding enzymes of Analysis of expression of the structural gene fused with the and promoters revealed that the biotin operon was subject to biotin-mediated feedback repression.


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