1887

Abstract

The nucleotide sequence of the biotin () operon of wild-type Sr41 was determined. Five ORFs were identified to encode BioA (7,8-diaminopelargonic acid aminotransferase), BioB (biotin synthase), BioF (7-keto-8-aminopelargonic acid synthase), BioC (an enzyme catalysing the synthesis of pimeloyl-CoA) and BioD (dethiobiotin synthase), in this order. The operon was deduced to be transcribed divergently to the left into and to the right into the genes. The promoters and a common predicted operator for both and genes were located between the and genes. The predicted amino acid sequences of these enzymes were similar to the sequences of the corresponding enzymes of Analysis of expression of the structural gene fused with the and promoters revealed that the biotin operon was subject to biotin-mediated feedback repression.

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1996-11-01
2024-12-08
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References

  1. Bachmann B. J. 1990; Linkage map of Escherichia coli K-12, edition 8. Microbiol Rev 54:130–194
    [Google Scholar]
  2. Barker D. F., Campbell A. 1981; Genetic and biochemical characterization of birA gene and its product: evidence for a direct role of biotin holoenzyme synthetase in repression of the biotin operon in Escherichia coli. J Mol Biol 153:469–492
    [Google Scholar]
  3. Boliver F., Rodriguez R. L., Greene P. J., Betlach H. L., Heynecker H. W., Boyer H. W., Crosa J. H., Falkow S. 1977; Construction and characterization of new cloning vehicles. II. A multipurpose cloning system. Gene 2:95–113
    [Google Scholar]
  4. Bradford M. M. 1976; A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of proteindye binding. Anal Biochem 72:248–254
    [Google Scholar]
  5. Davis B. D., Mingioli E. S. 1950; Mutants of Escherichia coli requiring methionine or vitamin B12. J Bacterial 60:17–28
    [Google Scholar]
  6. Demoll E., Shieve W. 1983; The origin of sulfur in biotin. Biochem Biophys Res Cornmm 110:243–249
    [Google Scholar]
  7. DeVries J. K., Zubay G. 1967; DNA-directed peptide synthesis, II. The synthesis of the α-fragment of the enzyme β-galactosidase. Proc Natl Acad Sci USA 57:267–287
    [Google Scholar]
  8. Eisenberg M. A. 1987; Biosynthesis of biotin and lipoic acid. In Escherichia coli and Salmonella typhimurium: Cellular and Molecular Biology,. pp. 544–550 Neidhardt F. C., Ingraham J. L., Brooks Low K., Magasanik B., Schaechter M., Umbarger H. E. Edited by Washington, DC: American Society for Microbiology.;
    [Google Scholar]
  9. Guha A. 1971; Divergent orientation of transcription from the biotin locus of Escherichia coli. J Mol Biol 56:53–62
    [Google Scholar]
  10. Harley C. B., Reynolds R. P. 1987; Analysis of E. coli promoter sequences. Nucleic Acids Res 15:2343–2361
    [Google Scholar]
  11. Hatakeyama K., Kohama K., Vertes A. A., Kobayashi M., Kurusu Y., Yukawa H. 1993; Analysis of the biotin biosynthesis pathway in coryneform bacteria: cloning and sequencing of the bioB gene from Brevibacterium flavum. DNA Seq 487–93
    [Google Scholar]
  12. Hayden M. A., Huang I., Bussiere D. E., Ashley G. W. 1992; The biosynthesis of lipoic acid. J Biol Chem 267:9512–9515
    [Google Scholar]
  13. Higgins D. G., Bleasby A. J., Fuchs R. 1992; CLUSTAL V: improved software for multiple sequence alignment. Comput Appl Biosci 8:189–191
    [Google Scholar]
  14. Hinnebusch A. G., Fink G. R. 1983; Repeated DNA sequences upstream from HIS1 also occur at several other co-regulated genes in Saccharomyces cerevisiae. J Biol Chem 258:5238–5247
    [Google Scholar]
  15. Ifuku O., Kishimoto J., Haze S., Yanagi M., Fukushima Y. 1992; Conversion of dethiobiotin to biotin in cell-free extracts of Escherichia coli. Biosci Biotechnol Biochem 56:1780–1785
    [Google Scholar]
  16. Ifuku O., Miyaoka H., Koga N., Kishimoto J., Haze, Wachi Y., Kajiwara M. 1994; Origin of carbon atoms of biotin 13C-NMR studies on biotin biosynthesis in Escherichia coli. Eur J Biochem 220:585–591
    [Google Scholar]
  17. Izumi Y., Morita H., Tani Y., Ogata K. 1974; The pimelyl-CoA synthetase responsible for the first step in biotin biosynthesis by microorganisms. Agric Biol Chem 38:2257–2262
    [Google Scholar]
  18. Komatsubara S. 1994; Amino acids: genetically engineered Serratia marcescens. In Recombinant Microbes for Industrial and Agricultural Applications pp. 467–484 Murooka Y., Imanaka T. Edited by New York: Marcel Dekker;
    [Google Scholar]
  19. Laemmli U. K. 1970; Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227:680–685
    [Google Scholar]
  20. Lisser S., Margalit H. 1993; Complication of E. coli mRNA promoter sequences. Nucleic Acids Res 21:1507–1516
    [Google Scholar]
  21. Lovenberg W. 1973 Iron-Sulfur Proteins Orlando, FL: Academic Press;
    [Google Scholar]
  22. Maniatis T., Fritsh E. F., Sambrook J. 1982 Molecular Cloning: a Laboratory Manual Cold Spring Harbor, NY: Cold Spring Harbor Laboratory;
    [Google Scholar]
  23. Mark S. G. 1978; The γδ sequence of F is an insertion sequence. J Mol Biol 126:346–365
    [Google Scholar]
  24. Masuda M., Takahashi K., Sakurai N., Yanagiya K., Komatsubara S., Tosa T. 1995; Further improvement of d-biotin production by a recombinant strain of Serratia marcescens. Process Biochem 30:553–562
    [Google Scholar]
  25. Matsumoto H., Tazaki T., Hosogaya S. 1973; A generalized transducing phage of Serratia marcescens. Jpn J Microbiol 17:473–479
    [Google Scholar]
  26. Miller i. H. 1972; Assay ofβ-galactosidase. In Experiments in Molecular Genetics Cold Spring Harbor, NY: Cold Spring Harbor Laboratory; pp. 352–355
    [Google Scholar]
  27. Omori K., Suzuki S., Imai Y., Komatsubara S. 1991; Analysis of the Serratia marcescens proBA operon and feedback control of proline biosynthesis. J Gen Microbiol 137:509–517
    [Google Scholar]
  28. Osawa I., Speck D., Kisou T., Hayakawa K., Zinsius M., Gloeckler R., Lemoine Y., Kamogawa K. 1989; Cloning of the biotin synthesis gene from Bacillus sphaericus and expression in E. coli and bacilli. Gene 80:39–40
    [Google Scholar]
  29. Otsuka A. J., Buoncristiani M. R., Howard P. K., Flamm J., Johnson C., R Y., Uchida K., Cook C., Ruppert J., Matsuzaki J. 1988; The Escherichia coli biotin biosynthetic enzyme sequences predicted from the nucleotide sequence of the bio operon. J Biol Chem 263:19577–19585
    [Google Scholar]
  30. Parry R. J. 1983; Biosynthesis of some sulfur containing natural products. Investigations of the mechanism of carbon-sulfur bond formation. Tetrahedron 39:1212–1238
    [Google Scholar]
  31. Pearson W. R., Lipman D. J. 1988; Improved tools for biological sequence comparison. Proc Natl Acad Sci USA 85:2444–2448
    [Google Scholar]
  32. Ploux O., Soularue P., Marquet A., Gloeckler R., Lemoine Y. 1992; Investigation of first step of biotin biosynthesis in Bacillus sphaericus. Biochem J 287:685–690
    [Google Scholar]
  33. Prakash O., Eisenberg M. A. 1979; Biotinyl 5′-adenylate: corepressor role in the regulation of the biotin genes of Escherichia coli K-12. Proc Natl Acad Sci USA 76:5592–5595
    [Google Scholar]
  34. Ringquist S., Shinedling S., Barrick D., Green L., Binkley J., Stormo G. D., Gold L. 1992; Translation initiation in Escherichia coli: sequences within the ribosome-binding site. Mol Microbiol 6:1219–1229
    [Google Scholar]
  35. Rosenberg M., Court D. 1984; Regulatory sequences involved in the promotion and termination of RNA transcription. Annu Rev Genet 13:319–353
    [Google Scholar]
  36. Sakurai N., Komatsubara S. 1996; Simple and versatile electrotransformation of Serratia marcescens. . Lett Appl Microbiol 23:23–26
    [Google Scholar]
  37. Sakurai N., Imai Y., Masuda M., Komatsubara M., Tosa T. (1993a); Construction of a biotin-overproducing strain of Serratia marcescens. Appl Environ Microbiol. Lett Appl Microbiol 59:2857–2863
    [Google Scholar]
  38. Sakurai N., Imai Y., Masuda M., Komatsubara M., Tosa T. (1993b); Molecular breeding of a biotin-hyperproducing Serratia marcescens strain. Appl Environ Microbiol 59:3225–3232
    [Google Scholar]
  39. Sakurai N., Imai Y., Masuda M., Komatsubara S., Tosa T. 1994; Improvement of a d-biotin-producing recombinant strain of Serratia marcescens. J Biotechnol 36:63–73
    [Google Scholar]
  40. Sanger F., Nicklen S., Coulson A. R. 1977; DNA sequencing with chain-terminating inhibitors. Proc Natl Acad Sci USA 74:5463–5467
    [Google Scholar]
  41. Sanyal I., Cohen G., Flint D. H. (1994a); Biotin synthase: purification, characterization as a [2Fe-2S] cluster protein, and in vitro activity of the Escherichia coli bioB gene product. Biochemistry 33:3625–3631
    [Google Scholar]
  42. Sanyal I., Lee S., Flint D. H. (1994b); Biosynthesis of pimeloyl- CoA, a biotin precursor in Escherichia coli, follows a modified fatty acid synthesis pathway: 13C-labeling studies. J Am Chem Soc 33:2637–2638
    [Google Scholar]
  43. Shiuan D., Campbell A. 1988; Transcriptional regulation and gene arrangement of Escherichia coli, Citrobacter freundii and Salmonella typhimurium biotin operons. Gene 67:203–211
    [Google Scholar]
  44. Stoker N. G., Fairweather N. F., Spratt B. G. 1982; Versatile low-copy-number plasmid vectors for cloning in Escherichia coli. Gene 18:335–341
    [Google Scholar]
  45. Takagi T., Kisumi M. 1985; Isolation of a versatile Serratia marcescens mutant as a host and molecular cloning of the aspartase gene. J Bacterial 161:1–6
    [Google Scholar]
  46. Takeshita S., Sato M., Toba M., Masahashi W., Hashimoto-Gotoh T. 1987; High-copy-number and low-copy-number plasmid vectors for /^cZ-complementation and chloramphenicol- or kanamycin-resistance selection. Gene 61:63–74
    [Google Scholar]
  47. Yanisch-Perron C., Vieira J., Messing J. 1985; Improved Ml 3 phage cloning vectors and host strains: nucleotide sequences of M13mpl8 and pUC19 vectors. Gene 33:103–119
    [Google Scholar]
  48. Zhang S., Sanyal I., Bulboaca G. H., Rich A., Flint D. H. 1994; The gene for biotin synthase from Saccharomyces cerevisiae: cloning, sequencing, and complementation of Escherichia coli strains lacking biotin synthase. Arch Biochem Biophys 309:29–35
    [Google Scholar]
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