The presence of proline in the medium was not essential for growth of indicating that there is a proline biosynthetic pathway in this organism. Genetic and biochemical analysis identified and characterized this pathway. Two genes, designated and were cloned, sequenced and characterized. Biochemical analysis of the - and -encoded enzymes showed that the proline biosynthetic pathway of is similar to the one previously described in The deduced amino acid sequence of a 2.408 kb DNA region containing the genes revealed the similarity of the gene products to ProB and ProA of and and to the corresponding N- and C-terminal domains of the bifunctional plant enzyme Δ-pyrroline-5-carboxylate synthetase of Northern blot analysis showed that the two genes in are organized in a single operon with proximal and distal to the promoter; primer extension analysis indicated that transcription is not under repressive control by exogenously supplied proline.


Article metrics loading...

Loading full text...

Full text loading...


Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error