1887

Abstract

The presence of proline in the medium was not essential for growth of indicating that there is a proline biosynthetic pathway in this organism. Genetic and biochemical analysis identified and characterized this pathway. Two genes, designated and were cloned, sequenced and characterized. Biochemical analysis of the - and -encoded enzymes showed that the proline biosynthetic pathway of is similar to the one previously described in The deduced amino acid sequence of a 2·408 kb DNA region containing the genes revealed the similarity of the gene products to ProB and ProA of and and to the corresponding N- and C-terminal domains of the bifunctional plant enzyme Δ-pyrroline-5-carboxylate synthetase of Northern blot analysis showed that the two genes in are organized in a single operon with proximal and distal to the promoter; primer extension analysis indicated that transcription is not under repressive control by exogenously supplied proline.

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1996-11-01
2021-08-06
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References

  1. Bae H.Y., Miller K.J. 1992; Identification of two proline transport systems in Staphylococcus aureusand their possible roles in osmoregulation. Appl Environ Microbiol 58:471–475
    [Google Scholar]
  2. Bardowski J., Ehrlich S.D., Chopin A. 1992; Tryptophan biosynthesis genes in Lactococcus lactis subsp.lactis. J Bacteriol 174:6563–6570
    [Google Scholar]
  3. Baumberg S., Klingel U. 1993; Biosynthesis of arginine, proline, and related compounds. In Bacillus subtilis and Other Grampositive Bacteria: Biochemistry, Physiology, and Molecular Genetics pp. 299–306 Sonenshein A.L., Hock J.A., Losick R. Edited by Washington, DC: American Society for Microbiology;
    [Google Scholar]
  4. Beumer R.R., Te Giffel M.C., Cox L.J., Rombouts F.M., Abee T. 1994; Effect of exogenous proline, betaine, and carnitine on growth of Listeria monocytogenes in minimal medium. Appl Environ Microbiol 60:1359–1363
    [Google Scholar]
  5. Bibb M.J., Janssen G.R., Ward J.M. 1986; Cloning and analysis of the promoter region of the erythromycin resistance gene(ermE)of Streptomyces erythraeus. Gene 41:E357–E368
    [Google Scholar]
  6. Bradford M.M. 1976; A rapid and sensitive method for the quantitation of microgram quantities of protein udlizing the principle of protein-dye binding. Anal Biochem 72:248–254
    [Google Scholar]
  7. Brady R.A., Csonka L.N. 1988; Transcriptional regulation of the proC gene of Salmonella typhimurium. . J Bacteriol 170:2379–2382
    [Google Scholar]
  8. Campanile C., Forlani G., Basso A.L., Marasco R., Ricca E., Sacco M., Ferrara L., De Felice M. 1993; Identification and characterization of the proBA operon of Streptococcus bovis. Appl Environ Microbiol 59:519–522
    [Google Scholar]
  9. Csonka L.N. 1989; Physiological and genetic responses of bacteria to osmotic stress. Microbiol Rev 53:121–147
    [Google Scholar]
  10. Cutting S., Panzer S., Losick R. 1989; Regulatory studies on the promoter for a gene governing synthesis and assembly of the spore coat in Bacillus subtilis. J Mol Biol 207:393–404
    [Google Scholar]
  11. Delorme C., Ehrlich S.D., Renault P. 1992; Histidine biosynthesis genes in Lactococcus lactis subsp.lactis. J Bacteriol 174:6571–6579
    [Google Scholar]
  12. Delorme C., Godon J.J., Ehrlich S.D., Renault P. 1993; Gene inactivation in Lactococcus lactis:histidine biosynthesis. J Bacteriol 175:4391–4399
    [Google Scholar]
  13. Deutch A.H., Rushlow K.E., Smith C.J. 1984; Analysis of the Escherichia coli proBA locus by DNA and protein sequencing. Nucleic Acids Res 12:6337–6355
    [Google Scholar]
  14. Fons M., Cami B., Patte J.C., Chippaux M. 1987; Cloning in Escherichia coli of genes involved in the synthesis of proline and leucine in Desulfovibrio desulfuricans Norway. Mol Gen Genet 206:141–143
    [Google Scholar]
  15. Godon J.J., Chopin M.C., Ehrlich S.D. 1992; Branched-chain amino acid biosynthesis genes in Lactococcus lactis subsp.lactis. J Bacteriol 174:6580–6589
    [Google Scholar]
  16. Hanahan D. 1983; Studies on transformation of Escherichia coli with plasmids. J Mol Biol 166:557–580
    [Google Scholar]
  17. Hayzer D.J., Moses V. 1978; The enzymes of proline biosynthesis in Escherichia coli. Biochem J 173:219–228
    [Google Scholar]
  18. Hopwood D.A., Bibb M.J., Chater K.F., Kieser T.C., Bruton J., Kieser H.M., Lydiate D.J., Smith C.P., Ward J.M., Schrempf M. 1985 Genetic Manipulation of Streptomyces: a Laboratory Manual. Norwich: John Innes Foundation;
    [Google Scholar]
  19. Horinouchi S., Furuya K., Nishiyama M., Suzuki H., Beppu T. 1987; Nucleotide sequence of the streptothricin acetyltransferase gene from Streptomyces lavendulae and its expression in heterologous hosts. J Bacteriol 169:1929–1937
    [Google Scholar]
  20. Hu C.-A., Delauney A.J., Verma D.P.S. 1992; A bifunctional enzyme (Δ1-pyrroline-5-carboxylate synthetase) catalyzes the first two steps in proline biosynthesis in plants. Proc Natl Acad Sci USA 899354–9358
    [Google Scholar]
  21. Igo M., Losick R. 1986; Regulation of a promoter that is utilized by minor forms of RNA polymerase holoenzyme in Bacillus subtilis. J Mol Biol 191:615–624
    [Google Scholar]
  22. Janssen G.R., Ward J.M., Bibb M.J. 1989; Unusual transcriptional and translational features of the aminoglycoside phosphotransferase gene(aph)from Streptomycesfradiae. Genes Dev 3:415–429
    [Google Scholar]
  23. Jewell J.B., Kashket E.R. 1991; Osmotically regulated transport of proline by Lactobacillus acidophilus IFO 3532. Appl Environ Microbiol 57:2829–2833
    [Google Scholar]
  24. Killham K., Firestone M.K. 1984; Salt stress control of intracellular solutes in Streptomyces indigenous to saline soils. Appl Environ Microbiol 47:301–306
    [Google Scholar]
  25. Klock G., Hillen W. 1986; Expression, purification and operator binding of the transposon Tn172T et-encoded Tet repressor. J Mol Biol 189:633–641
    [Google Scholar]
  26. Leisinger T. 1987; Biosynthesis of proline. . In Escherichia coli and Salmonella typhimurium: Cellular and Molecular Biology 1 pp. 345–351 Neidhardt F.C., Ingraham J.L., Brooks Low L., Magasanik B., Schaechter M., Umbarger H.E. Edited by Washington, DC: American Society for Microbiology;
    [Google Scholar]
  27. Limauro D., Avitabile A., Puglia A.M., Bruni C.B. 1992; Further characterization of the histidine gene cluster of Streptomyces coelicolor A3(2):nucleotide sequence and transcriptional analysis of hisD. . Res Microbiol 143:638–693
    [Google Scholar]
  28. Ludwig W., Kirchof G., Klugbauer N., Weizenegger M., Betzl D., Ehrmann M., Hertel C., Jilg S., Tatzel R., Zitzelsberger H., Liebl S., Hochberger M., Shah J., Lane D., Wallnoef P.R. 1992; Complete 23S ribosomal RNA sequences of Gram-positive bacteria with a low DNA G + C content. Syst Appl Microbiol 15:487–501
    [Google Scholar]
  29. Miller J.H. 1972 Experiments in Molecular Genetics p. 433 Cold Spring Harbor, NY: Cold Spring Harbor Laboratory;
    [Google Scholar]
  30. Molenaar D., Hagting A., Alkema H., Driessen A.J.M., Konings W.N. 1993; Characteristic and osmoregulatory roles of uptake systems for proline and glycine betaine in Lactococcus lactis. J Bacteriol 175:5438–5444
    [Google Scholar]
  31. Omori K., Suzuki S., Imai Y., Komatsubara S. 1991; Analysis of the Serratia marcescens proBA operon and feedback control of proline biosynthesis. J Gen Microbiol 137:509–517
    [Google Scholar]
  32. Ptashne M., Backman K., Humayun M.Z., Jeffrey A., Mauree R., Sauer R.T. 1976; Autoregulation and function of repressor in bacteriophage lambda. Science 194:156–161
    [Google Scholar]
  33. Reiter B., Oram J.D. 1962; Nutritional studies on cheese starters. I. Vitamin and amino acid requirements of single strain starters. J Daisy Res 29:63–77
    [Google Scholar]
  34. Sambrook J., Fritsch E.F., Maniatis T. 1989 Molecular Cloning: a Laboratory Manual, 2nd edn.. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory;
    [Google Scholar]
  35. Sanger F., Nicklen S., Coulson A.R. 1977; DNA sequencing with chain-terminating inhibitors. Proc Natl Acad Sci USA 745463–5467
    [Google Scholar]
  36. Slos P., Bourquin J.C., Lemoine Y., Mercenier A. 1991; Isolation and characterization of chromosomal promoters of Streptococcus salivarius subsp.thermophilus. Appl Environ Microbiol 57:1333–1339
    [Google Scholar]
  37. Smid E. J., Konings W. N. 1990; Relationship between utilization of proline and proline-containing peptides and growth of Lactococcus lactis. J Bacteriol 172:5286–5292
    [Google Scholar]
  38. Sprengart M.L., Fuchs E., Porter A.G. 1996; The downstream box: an efficient and independent translation initiation signal in Escherichia coli. EMBO J 15:665–674
    [Google Scholar]
  39. Stormo G., Schneider T., Gold L. 1982; Characterization of translational initiation sites in E. coli. Nucleic Acids Res 10:2971–2996
    [Google Scholar]
  40. Terzaghi B.E., Sandine W.E. 1975; Improved medium for lactic streptococci and their bacteriophages. Appl Microbiol 29:807–813
    [Google Scholar]
  41. Thomas P.S. 1980; Hybridization of denatured RNA and small DNA fragments transferred to nitrocellulose. Proc Natl Acad Sci USA 775201–5205
    [Google Scholar]
  42. de Vos W.M. 1987; Gene cloning and expression in lactic streptococci. FEMS Microbiol Rev 46:281–295
    [Google Scholar]
  43. Williams I., Frank L. 1975; Improved chemical synthesis and enzymatic assay of Δ1-pyrroline-5-carboxylic acid. Anal Biochem 64:85–97
    [Google Scholar]
  44. Yanisch-Perron C., Vieira J., Messing J. 1985; Improved Ml3 phage cloning vectors and host strains: nucleotide sequences of the M13mpl8 and pUC19 vectors. Gene 33:103–119
    [Google Scholar]
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