The structural gene () for succinyl-AMP (S-AMP) lyase and three additional ORFs are on the same DNA strand of the chromosome of Cassette mutagenesis and primer extension mapping demonstrated that is co-transcribed with an upstream gene (ORF23, or ) encoding a 22.9 kDa membrane-associated protein of non-essential, but unknown, function unrelated to purine biosynthesis. The operon lies between and an ORF expressing an essential function which may correspond to ). S-AMP lyase was purified to near homogeneity. The purified enzyme is a homotetramer of 50 kDa subunits, has a for S-AMP of 3.7 µM and a pH optimum of 7.4-7.6.


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