1887

Abstract

A two-dimensional (2-D) gel electrophoresis study of strain 168 identified 20 proteins that are strongly induced in response to phosphate starvation. The induction of nine of these phosphate-starvation-induced (Psi) proteins was dependent on a functional PhoR protein. PhoR is the histidine sensor-kinase component of a phosphate-concentration-sensing two-component regulatory system which, together with its partner response regulator PhoP, controls the expression of genes in the Pho regulon. Genes encoding PhoR-dependent Psi proteins are therefore likely to be members of the Pho regulon. SpoOA ~ P, the response regulator of the signal transduction pathway required for the induction of sporulation, has previously been shown to negatively affect the induction of the Pho regulon by repressing the operon. The induction pattern of some PhoR-dependent Psi proteins was altered in a mutant such that their synthesis continued for longer than was found with the wild-type. The most abundant Psi protein, Psi1–3, was characterized by N-terminal sequencing of internal peptide fragments and shown to have a high similarity to an protein which is involved in phosphate uptake during phosphate starvation.

Loading

Article metrics loading...

/content/journal/micro/10.1099/13500872-142-11-3163
1996-11-01
2021-10-23
Loading full text...

Full text loading...

/deliver/fulltext/micro/142/11/mic-142-11-3163.html?itemId=/content/journal/micro/10.1099/13500872-142-11-3163&mimeType=html&fmt=ahah

References

  1. Antelmann H., Bernhardt J., Schmid R., Hecker M. 1995; A gene at 333° on the Bacillus subtilis chromosome encodes the newly identified σB-dependent general stress protein GspA. J Bacteriol 177:3540–3545
    [Google Scholar]
  2. Ausubel F. M. 1993; Isolation of proteins for microsequence analysis, unit 10.19.1-10.19.11. In Current Protocols in Molecular Biology 2 Chichester: Wiley;
    [Google Scholar]
  3. Birkey S. M., Sun G., Hulett F. M. 1995; Three two-component signal transduction systems interact for PHO regulation in Bacillus subtilis. Abstracts of the 8th International Meeting on Bacilli Stanford University, USA
    [Google Scholar]
  4. Chesnut R. S., Bookstein C., Hulett F. M. 1991; Separate promoters direct expression of phoAIII, a member of the Bacillus subtilis alkaline phosphatase multigene family during phosphate starvation and sporulation. Mol Microbiol 5:2181–2190
    [Google Scholar]
  5. Coleman J. E. 1992; Structure and mechanism of alkaline phosphatase. Annu Rep Biophys Biomol Struct 21:441–483
    [Google Scholar]
  6. Grant W. D. 1979; Cell wall teichoic acid as a reserve phosphate source in Bacillus subtilis. J Bacteriol 137:35–43
    [Google Scholar]
  7. Hecker M., Voelker U. 1990; General stress proteins in B. subtilis. FEMS Microbiol Ecol 74:197–213
    [Google Scholar]
  8. Hulett F. M. 1993; Regulation of phosphorus metabolism. In Bacillus subtilis and Other Gram-positive Bacteria: Biochemistry, Physiology and Molecular Biology pp. 229–235 Edited by Sonenshein A. L., Hoch J. A., Losick R. Washington, DC: American Society for Microbiology;
    [Google Scholar]
  9. Hulett F. M., Bookstein C., Jenson K. 1990; Evidence for two structural genes for alkaline phosphatase in Bacillus subtilis. J Bacteriol 172:735–740
    [Google Scholar]
  10. Hulett F. M., Kim E. E., Bookstein C., Kapp N. V., Edwards C. W., Wyckoff H. W. 1991; Bacillus subtilis alkaline phosphatases III and IV. Cloning, sequencing, and comparisons of deduced amino acid sequence with Escherichia coli alkaline phosphatase three dimensional structure. J Biol Chem 266:1077–1084
    [Google Scholar]
  11. Hulett F. M., Lee J., Shi L., Sun G., Chesnut R. S., Sharkova E., Duggan M. F., Kapp N. 1994; Sequential action of two- component genetic switches regulates the PHO regulon in Bacillus subtilis. J Bacteriol 176:1348–1358
    [Google Scholar]
  12. Igo M., Lampe M., Ray C., Schafer W., Moran C. P.Jr Losick R. 1987; Genetic studies of a secondary RNA polymerase sigma factor in Bacillus subtilis. J Bacteriol 169:3463–3469
    [Google Scholar]
  13. Jensen K. K., Sharkova E., Duggan M. F., Qi Y., Koide A., Hoch J. A., Hulett F. M. 1993; Bacillus subtilis transcription regulator, SpoOA, decreases alkaline phosphatase levels induced by phosphate starvation. J Bacteriol 175:3749–3756
    [Google Scholar]
  14. Matsudaira P. T. 1989 A Practical Guide to Protein and Peptide Purification for Microsequencing. San Diego: Academic Press;
    [Google Scholar]
  15. Nicholson W. L., Setlow P. 1990; Sporulation, germination and outgrowth. In Molecular Biological Methods for Bacillus pp. 391–450 Edited by Harwood C. R., Cutting S. M. Chicester: Wiley;
    [Google Scholar]
  16. Rao N. N., Torriani A. 1990; Molecular aspects of phosphate transport in Escherichia coli. Mol Microbiol 4:1083–1090
    [Google Scholar]
  17. Sambrook J., Fritsch E. F., Maniatis T. 1989 Molecular Cloning: a Laboratory Manual, 2nd edn.. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory;
    [Google Scholar]
  18. Seki T., Yoshikawa H., Takahashi H., Saito H. 1988; Nucleotide sequence of the Bacillus subtilis phoR. gene. J Bacteriol 170:5935–5938
    [Google Scholar]
  19. Shi L., Hulett F. M. 1995; PhoR, the histidine sensor-kinase of the two-component signal transduction system of Bacillus subtilis. Abstracts of the 8th International Meeting on Bacilli Stanford University, USA
    [Google Scholar]
  20. Stülke J., Hanschke R., Hecker M. 1993; Temporal activation of β-glucanase synthesis in Bacillus subtilis is mediated by the GTP pool. J Gen Microbiol 139:2041–2045
    [Google Scholar]
  21. Sun G. F., Birkey S. M., Hulett F. M. 1996; Three two-component signal-transduction systems interact for Pho regulation in Bacillus subtilis. Mol Microbiol 19:941–948
    [Google Scholar]
  22. Völker U., Engelmann S., Maul B., Riethdorf S., VÖlker A., Schmid R., Mach H., Hecker M. 1994; Analysis of the induction of general stress proteins of Bacillus subtilis. Microbiol 140:741–752
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/13500872-142-11-3163
Loading
/content/journal/micro/10.1099/13500872-142-11-3163
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error