1887

Abstract

As part of the genome sequencing project, we have determined a 283 kb contiguous sequence from 210° to 232° of the genome. This region contains the 48 kb element which is excised during sporulation by a site-specific recombinase. In this region, 310 complete ORFs and one tRNA gene were identified: 66 ORFs have been sequenced and characterized previously by other workers, e.g. and operons; and genes; many sporulation genes and operons, and etc. The products of 84 ORFs were found to display significant similarity to proteins with known function in data banks, e.g., proteins involved in nucleotide metabolism, lipid biosynthesis, amino acid transport (ABC transporter), phosphate-specific transport, the glycine cleavage system, the two-component regulatory system, cell wall autolysis, ferric uptake and sporulation. However, the functions of more than half of the ORFs (52%, 160 ORFs) are still unknown. In the element containing 60 ORFs, 32 ORFs (53%) encode proteins which have significant homology to gene products of the temperate phage 105 and/or the defective phage PBSX.

Loading

Article metrics loading...

/content/journal/micro/10.1099/13500872-142-11-3103
1996-11-01
2021-07-31
Loading full text...

Full text loading...

/deliver/fulltext/micro/142/11/mic-142-11-3103.html?itemId=/content/journal/micro/10.1099/13500872-142-11-3103&mimeType=html&fmt=ahah

References

  1. Barnes W. M. 1994; PCR amplification of up to 35-kb DNA with high fidelity and high yield from X bacteriophage templates. Proc Natl Acad Set USA 912216–2220
    [Google Scholar]
  2. Burland V., Plunkett G.III Sofia H. J., Daniels D. L., Blattner F. R. 1995; Analysis of the Escherichia coli genome VI: DNA sequence of the region from 92·8 through 100 minutes. Nucleic Acids Res 23:2105–2119
    [Google Scholar]
  3. DiRusso C. C. 1990; Primary sequence of Escherichia coli fabBA operon, encoding the fatty acid-oxidizing multienzyme complex, indicates a high degree of homology to eucaryotic enzymes. J Bacteriol 172:6459–6468
    [Google Scholar]
  4. Errington J. 1993; Bacillus subtilis sporulation: regulation of gene expression and control of morphogenesis. Microbiol Rev 57:1–33
    [Google Scholar]
  5. Errington J., Appleby L., Daniel R. A., Goodfellow H., Partridge S. R., Yudkin M. D. 1992; Structure and function of the spoIIIJ gene of Bacillus subtilis: a vegetatively expressed gene that is essential for σG activity at an intermediate stage of sporulation. J Gen Microbiol 138:2609–2618
    [Google Scholar]
  6. Fleischmann R. D. and others 1995; Whole-genome random sequencing and assembly of Haemophilus influenzae Rd. Science 269:496–512
    [Google Scholar]
  7. Fraser C. M. and others 1995; The minimal gene complement of Mycoplasma genitalium. Science 270:397–403
    [Google Scholar]
  8. Hori H., Osawa S. 1987; Origin and evolution of organisms as deduced from 5S ribosomal RNA sequences. Mol Biol Evol 4:445–472
    [Google Scholar]
  9. Hourdou M. -L., Guinand M., Vacheron M. -J., Michel G., Denoroy L., Duez C., Englebert S., Joris B., Weber G., Ghuysen J. -M. 1993; Characterization of the sporulation-related γ-d-glutamyl-(l)meso-diaminopimelic-acid-hydrolysing peptidase I of Bacillus sphaericus NCTC 9602 as a member of the metallo (zinc) carboxypeptidase A family. Biochem J 292:563–570
    [Google Scholar]
  10. Itaya M., Tanaka T. 1991; Complete physical map of the Bacillus subtilis 168 chromosome constructed by a gene-directed mutagenesis method. J Mol Biol 220:631–648
    [Google Scholar]
  11. Kaneko T. and others 1995; Sequence analysis of the genome of the unicellular cyanobacterium Synechocystis sp. strain PCC 68033.I. Sequence features in the 1 Mb region from map positions 64 % to 92% of the genome. DNA Res 2:153–166
    [Google Scholar]
  12. Krogh S., O´Reilly M., Nolan N., Devine K. M. 1996; The phage-like element PBSX and part of the skin element, which are resident at different locations on the Bacillus subtilis chromosome, are highly homologous. Microbiology 142:2031–2040
    [Google Scholar]
  13. Kunst F., Vassarotti A., Danchin A. 1995; Organization of the European Bacillus subtilis genome sequencing project. Microbiology 141:249–255
    [Google Scholar]
  14. Kuroda A., Sekiguchi J. 1990; Cloning, sequencing and genetic mapping of a Bacillus subtilis cell wall hydrolase gene. J Gen Microbiol 136:2209–2216
    [Google Scholar]
  15. Kuroda A., Sekiguchi J. 1991; Molecular cloning and sequencing of a major Bacillus subtilis autolysin gene. J Bacteriol 173:7304–7312
    [Google Scholar]
  16. Meinhardt F., BuBkamp M., Wittchen K. D. 1994; Cloning and sequencing of the leuC and nprM genes and a putative spoIV gene from Bacillus megaterium DSM319. Appl Microbiol Biotechnol 41:344–351
    [Google Scholar]
  17. Nohno T., Saito T., Hong J. -S. 1986; Cloning and complete nucleotide sequence of the Escherichia coli glutamine permease operon glnHPQ). Mol Gen Genet 205:260–269
    [Google Scholar]
  18. Ochman H., Gerber A. S., Hartl D. L. 1988; Genetic application of an inverse polymerase chain reaction. Genetics 120:621–623
    [Google Scholar]
  19. Ogasawara N., Nakai S., Yoshikawa H. 1994; Systematic sequencing of the 180 kilobase region of the Bacillus subtilis chromosome containing the replication origin. DNA Res 1:1–14
    [Google Scholar]
  20. Ogasawara N., Fujita Y., Kobayashi Y., Sadaie Y., Tanaka T., Takahashi H., Yamane K., Yoshikawa H. 1995; Systematic sequencing of the Bacillus subtilis genome: progress report of the Japanese group. Microbiology 141:257–259
    [Google Scholar]
  21. O-lkeda K., Ohmura Y., Fujikawa K., Motokawa Y. 1993; Cloning and nucleotide sequencing of the gev operon encoding the Escherichia coli glycine-cleavage enzyme system. Eur J Biochem 216:539–548
    [Google Scholar]
  22. Sambrook J., Fritsch E. F., Maniatis T. 1989 Molecular Cloning: a Eaboratory Manual. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory;
    [Google Scholar]
  23. Schäffer S., Hantke K., Braun V. 1985; Nucleotide sequence of the iron regulatory gene fur. Mol Gen Genet 200:110–113
    [Google Scholar]
  24. Stock J. B., Ninfa A. J., Stock A. M. 1989; Protein phosphorylation and regulation of adaptive responses in bacteria. Microbiol Rev 53:450–490
    [Google Scholar]
  25. Surin B. P., Rosenberg H., Cox G. B. 1985; Phosphate-specific transport system of Escherichia coli: nucleotide sequence and genepolypeptide relationships. J Bacteriol 161:189–198
    [Google Scholar]
  26. Takemaru K., Mizuno M., Sato T., Takeuchi M., Kobayashi Y. 1995; Complete nucleotide sequence of a skin element excised by DNA rearrangement during sporulation in Bacillus subtilis. Microbiology 141:323–327
    [Google Scholar]
  27. Takemaru K., Mizuno M., Kobayashi Y. 1996; A Bacillus subtilis gene cluster similar to the Escherichia coli phosphate-specific transport (pst) operon: evidence for a tandemly arranged pstB gene. Microbiology 142:2017–2020
    [Google Scholar]
  28. V-Hansen P., Hammer K., Larsen J. E. L., Svendsen I. 1984; The internal regulated promoter of the deo operon of Escherichia coli K-12. Nucleic Acids Res 12:5211–5224
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/13500872-142-11-3103
Loading
/content/journal/micro/10.1099/13500872-142-11-3103
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error