1887

Abstract

acquired increased acid tolerance during exponential growth upon exposure to sublethal acid stress, a response designated the acid tolerance response (ATR). Maximal acid resistance was seen when the organism was exposed to pH 5·0 for 1 h prior to challenge at pH 3·0, although intermediate levels of protection were afforded by exposure to pH values ranging from 4·0 to 6·0. A 60 min adaptive period was required for the development of maximal acid tolerance; during this period the level of acid tolerance increased gradually. Full expression of the ATR required protein synthesis; chloramphenicol, a protein synthesis inhibitor, prevented full induction of acid tolerance. Analysis of protein expression during the adaptive period by two-dimensional gel electrophoresis revealed a change in the expression of at least 23 proteins compared to the non-adapted culture. Eleven proteins showed induced expression while 12 were repressed, implying that the ATR is a complex response involving a modulation in the expression of a large number of genes. In addition to the exponential phase ATR, also developed increased acid resistance upon entry into the stationary phase; this response appeared to be independent of the pH-dependent ATR seen during exponential growth.

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1996-10-01
2021-08-03
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References

  1. Abrams A., Jensen C. 1984; Altered expression of the H+ ATPase in Streptococcus faecalis membranes.. Biochem Biophys Res Commun 122:151–157
    [Google Scholar]
  2. Arnold K.W., Kasper C.W. 1995; Starvation- and stationary-phase-induced acid tolerance in Escherichia coli O157 :H7. Appl Environ Microbiol 61:2037–2039
    [Google Scholar]
  3. Belli W.A., Marquis R.E. 1991; Adaptation of Streptococcus mutans and Enterococcus hirae to acid stress in continuous culture. Appl Environ Microbiol 57:1134–1138
    [Google Scholar]
  4. Bender G.R., Sutton S.V.W., Marquis R.E. 1986; Acid tolerance, proton permeabilities, and membrane ATPases of oral streptococci. Infect Immun 53:331–338
    [Google Scholar]
  5. Casiano-Colón A., Marquis R.E. 1988; Role of the arginine deiminase system in protecting oral bacteria and an enzymatic basis for acid tolerance. Appl Environ Microbiol 54:1318–1324
    [Google Scholar]
  6. Cole M.B., Jones M.V., Holyoak C. 1990; The effect of pH, salt concentration and temperature on the survival and growth of Listeria monocytogenes . J Appl Bacteriol 69:63–72
    [Google Scholar]
  7. Conner D.E., Scott V.N., Bernard D.T. 1990; Growth, inhibition and survival of Listeria monocytogenes as affected by acidic conditions. J Food Prot 53:652–655
    [Google Scholar]
  8. Foster J.W. 1991; Salmonella acid shock proteins are required for the adaptive acid tolerance response. J Bacteriol 173:6896–6902
    [Google Scholar]
  9. Foster J.W., Hall H.K. 1990; Adaptive acidification tolerance response of Salmonella typhimurium . J Bacteriol 172:771–778
    [Google Scholar]
  10. Foster J.W., Hall H.K. 1991; Inducible pH homeostasis and the acid tolerance response of Salmonella typhimurium . J Bacteriol 173:5129–5135
    [Google Scholar]
  11. Goodson M., Rowbury R. J. 1989a; Habituation to normally lethal acidity by prior growth of Escherichia coli at a sub-lethal acid pH value. Lett Appl Microbiol 8:77–79
    [Google Scholar]
  12. Goodson M., Rowbury R. J. 1989b; Resistance of acid habituated Escherichia coli to organic acids and its medical and applied significance. Lett Appl Microbiol 8:211–214
    [Google Scholar]
  13. Gorden J., Small P.LC. 1993; Acid resistance in enteric bacteria. Infect Immun 61:364–367
    [Google Scholar]
  14. Hengge-Aronis R. 1993; Survival of hunger and stress: the role of rpoS in early stationary phase gene regulation in E. coli . Cell 72:165–168
    [Google Scholar]
  15. Hickey E.W., Hirshfield I.N. 1990; Low-pH-induced effects on patterns of protein synthesis and on internal pH in Escherichia coliand Salmonella typhimurium . Appl Environ Microbiol 56:1038–1045
    [Google Scholar]
  16. Karem K.L., Foster J.W., Bej A.K. 1994; Adaptive acid tolerance response (ATR) in Aeromonas hydrophila . Microbiology 140:1731–1736
    [Google Scholar]
  17. Kobayashi H., Suzuki T., Kinoshita N., Unemoto T. 1984; Amplification of the Streptococcus faecalis proton-translocating ATPase by a decrease in cytoplasmic pH. J Bacteriol 158:1157–1160
    [Google Scholar]
  18. Kobayashi H., Suzuki T., Unemoto T. 1986; Streptococcal cytoplasmic pH is regulated by changes in amount and activity of a proton-translocating ATPase. J Biol Chem 261:627–630
    [Google Scholar]
  19. Kroll R.G., Patchett R.A. 1992; Induced acid tolerance in Listeria monocytogenes . Lett Appl Microbiol 14:224–227
    [Google Scholar]
  20. Lee I.S., Slonczewski J.L., Foster J.W. 1994; A low-pH- inducible, stationary-phase acid tolerance response in Salmonella typhimurium . J Bacteriol 176:1422–1426
    [Google Scholar]
  21. Lin J., Lee I.S., Frey J., Slonczewski J.L., Foster J.W. 1995; Comparative analysis of extreme acid survival in Salmonella typhimurium, Shigella flexneri, and Escherichia coli . J Bacteriol 177:4097–4104
    [Google Scholar]
  22. McClure P.J., Roberts T.A., Oguru P.O. 1989; Comparison of the effects of sodium chloride, pH, and temperature on the growth of Listeria monocytogenes on gradient plates and in liquid medium. Lett Appl Microbiol 9:95–99
    [Google Scholar]
  23. O’Farrell P.H. 1975; High resolution two-dimensional electrophoresis of proteins. J Biol Chem 250:4007–4021
    [Google Scholar]
  24. Raja N., Goodson M., Chui W.C.M., Smith D.G., Rowbury R.J. 1991; Habituation to acid in Escherichia coli: conditions for habituation and its effects on plasmid transfer. J Appl Bacteriol 70:59–65
    [Google Scholar]
  25. Rowbury R.J. 1995; An assessment of environmental factors influencing acid tolerance and sensitivity in Escherichia coli, Salmonella spp. and other enterobacteria. Lett Appl Microbiol 20:333–337
    [Google Scholar]
  26. Rowbury R.J., Goodson M. 1993; PhoE porin of Escherichia coli and phosphate reversal of acid damage and killing and of acid induction of the CadA gene product. J Appl Bacteriol 74:652–661
    [Google Scholar]
  27. Small P., Blankenhorn D., Welty D., Zinser E., Slonczewski J.L. 1994; Acid base resistance in Escherichia coli and Shigella flexneri: role of rpoS and growth pH. J Bacteriol 176:1729–1737
    [Google Scholar]
  28. Young K.M., Foegeding P.M. 1993; Acetic, lactic and citric acids and pH inhibition of Listeria monocytogenes Scott A and the effect of intracellular pH. J Appl Bacteriol 74:515–520
    [Google Scholar]
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