1887

Abstract

A soluble mono(ADP-ribosyl)transferase was detected in dormant spores of Soluble proteins incubated with [P]NAD revealed, after two-dimensional electrophoresis, three major ADP-ribosylated substrates with molecular masses of 38, 37 and 36 kDa and pl values of 6·9, 8·1 and 4·6, respectively. When these endogenous substrates were first ADP-ribosylated with [P]NAD and then incubated for different times with either 3 M hydroxylamine (pH 7·0) or 1 mM HgCl, only hydroxylamine released the incorporated radioactivity after 30 min incubation. Additionally, agmatine was used as a substrate for this enzyme. These data suggest that the mono(ADP-ribosyl)transferase is an arginine-specific enzyme. This enzymic activity was stimulated by 10 mM MgCl and by 250 μM of the nitric-oxide-releasing agent sodium nitroprusside, and inhibited by 8 mM benzamide, 0·4 mM -iodobenzylguanidine and 0·5 mM novobiocin. The three ADP-ribosylation inhibitors affected the germination of spores, leaving them as swollen cells. The effect of MgCl, GTP and ATP on the ADP-ribosylation of the endogenous proteins was studied. The presence of two additional [P]ADP-ribosylated proteins of 57 and 55 kDa was observed in the absence of MgCl. An increase in incorporation of radioactivity into the 55 kDa band was observed when the assay was performed in the presence of GTP or ATP. The addition of Mg together with either or both nucleotides eliminated the appearance of the 57 and 55 kDa bands, but intensified the 37 kDa band. Photoaffinity-labelling of the soluble fraction with [α-P]GTP revealed a 55 kDa band together with other proteins of 32 and 17 kDa. These results suggest that among the five different endogenous substrates for the fungal mono(ADP-ribosyl)transferase, the 55 kDa protein may be a GTP-binding protein.

Loading

Article metrics loading...

/content/journal/micro/10.1099/13500872-142-10-2907
1996-10-01
2021-08-01
Loading full text...

Full text loading...

/deliver/fulltext/micro/142/10/mic-142-10-2907.html?itemId=/content/journal/micro/10.1099/13500872-142-10-2907&mimeType=html&fmt=ahah

References

  1. Banasik M., Komura H., Shimoyama M., Ueda K. 1992; Specific inhibitors of poly(ADP-ribose) synthetase and mono(ADP- ribosyl)transferase. . J Biol Chem 267:1569–1575
    [Google Scholar]
  2. Bartnicki-Garcia S., Nickerson W.J. 1962; Nutrition, growth, and morphogenesis of Mucor rouxii. . J Bacterial 84:841–858
    [Google Scholar]
  3. Bravo R. 1984; Two dimensional gel electrophoresis : a guide for the beginner. . In Two-dimensional Gel Electrophoresis of Proteins pp. 3–36 Celis J.E., Bravo R. Edited by New York: Academic Press;
    [Google Scholar]
  4. Brüne B., Lapetina E.G. 1989; Activation of cytosolic ADP- ribosyltransferase by nitric oxide-generating agents.. J Biol Chem 264:8455–8458
    [Google Scholar]
  5. Brüne B., Molina y Vedia L., Lapetina E.G. 1990; Agonist induced ADP-ribosylation of a cytosolic protein in human platelets.. Proc Natl Acad Sci USA 873304–3308
    [Google Scholar]
  6. Carrillo-Rayas M.T., García-Soto J., Martínez-Cadena G. 1988; 12-O-Tetradecanoyl phorbol-13-acetate interferes with germination of Phycomyces blakesleeanus sporangiospores. FEBS Lett 238:441–444
    [Google Scholar]
  7. De Matteis M.A., DiGirolamo M., Colanzi A., Pallas M., DiTullio G., McDonald L.S., Moss J., Santini G., Bannykh S., Corda D., Luini A. 1994; Stimulation of endogenous ADP- ribosylation by brefeldin A.. Proc Natl Acad Sci USA 911114–1118
    [Google Scholar]
  8. Hasunuma K. 1991; Nucleoside triphosphate (NTP)-binding proteins and endogenous ADP-ribosyl transferase inNeurospora crassa . Plant Cell Physiol 32:653–664
    [Google Scholar]
  9. Jacobson M.K., Loflin P.T., Aboul-Ela N., Minmuang M., Moss J., Jacobson E.L. 1990; Modification of plasma membrane protein cysteine residues by ADP-ribosein vivo . J Biol Chem 265:10825–10828
    [Google Scholar]
  10. Laemmli U.K. 1970; Cleavage of structural proteins during the assembly of the head of bacteriophage T4.. Nature 227:680–685
    [Google Scholar]
  11. McDonald L.J., Moss J. 1993; Nitric oxide-independent, thiol- associated ADP-ribosylation inactivates aldehyde dehydrogenase. J Biol Chem 268:17878–17882
    [Google Scholar]
  12. Martínez-Cadena G., Novoa-Martínez G., González-Hernández A., García-Soto J. 1995; The GTP-binding protein Gα sis present in dormant spores and expressed differentially during spore germination of the fungus Phycomyces blakesleeanus . Microbiology 141:3149–3154
    [Google Scholar]
  13. Meyer T., Koch R., Fanick W., Hilz H. 1988; ADP-ribosyl proteins formed by pertussis toxin are specifically cleaved by mercury ions. Biol Chem Hoppe-Seyler 369:579–583
    [Google Scholar]
  14. Molina y Vedia L., Nolan R.D., Lapetina E.G. 1989; The effect of iloprost on the ADP-ribosylation of Gsα (the α-subunit of Gs). Biochem J 261:841–845
    [Google Scholar]
  15. Moss J., Yost A., Stanley S.J. 1983; Amino acid-specific ADP- ribosylation. J Biol Chem 258:6466–6470
    [Google Scholar]
  16. Moss J., Jacobson M.K., Stanley S.J. 1985; Reversibility of arginine-specific mono(ADP-ribosylation) : identification in erythrocytes of an ADP-ribose-l-arginine cleavage enzyme. Proc Natl Acad Sci USA 825603–5607
    [Google Scholar]
  17. Riquelme P.T., Burzio L.O., Koide S.S. 1979; ADP- ribosylation of rat liver lysine-rich histonein vitro . J Biol Chem 254:3018–3028
    [Google Scholar]
  18. Rivero F., Cerdá-Olmedo E. 1987; Spore activation by acetate, propionate and heat in Phycomycesmutants. Mol Gen Genet 209:149–153
    [Google Scholar]
  19. Smets L.A., Loesberg C., Janssen M., Van Rooij H. 1990; Intracellular inhibition of mono(ADP-ribosylation) bymeta-iodo- benzylguanidine : specificity, intracellular concentration and effects on glucocorticoid-mediated cell lysis. Biochim Biophys Acta 1054:49–55
    [Google Scholar]
  20. Soman G., Mickelson S.J., Louis C.F., Graves D.J. 1984; NAD:guanidino group specific mono ADP-ribosyltransferase activity in skeletal muscle. Biochem Biophys Res Commun 120:973–980
    [Google Scholar]
  21. Sutter R.P. 1975; Mutations affecting sexual development in Phycomyces blakesleeanus . Proc Natl Acad Set USA 72127–130
    [Google Scholar]
  22. Tanigawa Y., Tsuchiya M., Imai Y., Shimoyama M. 1984; ADP-ribosyltransferase from hen liver nuclei. J Biol Chem 259:2022–2029
    [Google Scholar]
  23. Tao Y., Howlett A., Klein C. 1992; Nitric oxide stimulates the ADP-ribosylation of a 41-kDa cytosolic protein in Dictyostelium discoideum. . Proc Natl Acad Sci USA 895902–5906
    [Google Scholar]
  24. Van Laere A.J., Hendrix P. 1983; Cyclic AMP-dependentin vitroactivation of trehalase from dormant Phycomyces blakesleeanusspores. J Gen Microbiol 129:3287–3290
    [Google Scholar]
  25. Van Laere A.J., Rivero F. 1986; Properties of a germination mutant of Phycomyces blakesleeanus. . Arch Microbiol 145:290–294
    [Google Scholar]
  26. Van Laere A.J., Van Assche J.A., Furch B. 1987; The sporangiospore : dormancy and germination. . In Phycomyces pp. 247–279 Cerdá-Olmedo E., Lipson E.D. Edited by Cold Spring Harbor, NY: Cold Spring Harbor Laboratory;
    [Google Scholar]
  27. Van Mulders R.M., Van Laere A.J. 1984; Cyclic AMP, trehalase and germination of Phycomyces blakesleeanusspores. J Gen Microbiol 130:541–547
    [Google Scholar]
  28. Williamson K.C., Moss J. 1990; Mono-ADP-ribosyl- transferases and ADP-ribosylarginine hydrolases: a mono-ADP ribosylation cycle in animal cells . In ADP-ribosylating Toxins and G Proteins: Insights into Signal Transduction pp. 493–510 Moss J., Vaughan M. Edited by Washington, DC: American Society for Microbiology;
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/13500872-142-10-2907
Loading
/content/journal/micro/10.1099/13500872-142-10-2907
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error