1887

Abstract

The response of to the electrophile -ethylmaleimide (NEM) has been investigated. Treatment with low levels of NEM (20–30 μM) led to transient growth inhibition followed by recovery of normal growth. Stationary phase cells acquired resistance to NEM; one component of this tolerance was a more rapid metabolism of NEM. In exponential phase cells, the period of growth inhibition was associated with detoxification of NEM. Detoxification was biphasic and cells lost the ability to form colonies on minimal agar plates during the first phase of detoxification. Full viability was retained on MacConkey agar. Peptones are the active components in MacConkey medium. Addition of peptones to minimal agar restored colony-forming ability, but each peptone source had a different efficiency. These data suggest that a deficiency in the ability to assimilate nitrogen is a consequence of NEM treatment.

Loading

Article metrics loading...

/content/journal/micro/10.1099/13500872-142-10-2857
1996-10-01
2021-05-08
Loading full text...

Full text loading...

/deliver/fulltext/micro/142/10/mic-142-10-2857.html?itemId=/content/journal/micro/10.1099/13500872-142-10-2857&mimeType=html&fmt=ahah

References

  1. Bakker E.P., Booth I.R., Dinnbier U., Epstein W., Gajewska A. 1987; Evidence for multiple K+-export systems in Escherichia coli . J Bacterial 169:3743–3749
    [Google Scholar]
  2. Cooper R.A. 1984; Metabolism of methylglyoxal in microorganisms. Annu Rev Microbiol 38:49–68
    [Google Scholar]
  3. Douglas R.M., Roberts J.A., Munro A.W., Ritchie G.Y., Lamb A.J., Booth I.R. 1991; The distribution of homologues of the Escherichia coli KefC K+-efflux system in other bacterial species. J Gen Microbiol 137:1999–2005
    [Google Scholar]
  4. Eberl L., Givskov M., Sternberg C., Møller S., Christiansen G., Molin S. 1996; Physiological responses of Pseudomonas putida KT2442 to phosphate starvation. Microbiology 142:155–163
    [Google Scholar]
  5. Elmore M.J., Lamb A.J., Ritchie G.Y., Douglas R.M., Munro A., Gajewska A., Booth I.R. 1990; Activation of potassium efflux from Escherichia coli by glutathione metabolites. Mol Microbiol 4:405–412
    [Google Scholar]
  6. Epstein W., Kim B.S. 1971; Potassium transport loci in Escherichia coli K-12. J Bacteriol 108:639–644
    [Google Scholar]
  7. Ferguson G.P., Munro A.W., Douglas R.M., McLaggan D., Booth I.R. 1993; Activation of potassium channels during metabolite detoxification in Escherichia coli . Mol Microbiol 9:1–7
    [Google Scholar]
  8. Ferguson G.P., McLaggan D. 1995; Potassium channel activation by glutathione-S-conjugates in Escherichia coli: protection against methylglyoxal is mediated by cytoplasmic acidification. Mol Microbiol 17:1025–1033
    [Google Scholar]
  9. Givskov M., Møller S., Paulsen L. K., Molin S. 1994a; Responses to nutrient starvation in Pseudomonas putida KT2442: analysis of general cross-protection, cell shape, and macromolecular content. J Bacteriol 176:7–14
    [Google Scholar]
  10. Givskov M., Eberl L., Molin S. 1994b; Responses to nutrient starvation in Pseudomonas putida KT2442: two-dimensional electrophoretic analysis of starvation- and stress-induced proteins. J Bacteriol 176:4816–4824
    [Google Scholar]
  11. Gray D.I., Morris B.M. 1992; A low cost video analysis system for the BBC master computer. Binary 4:58–61
    [Google Scholar]
  12. Hengge-Aronis R. 1993; Survival of hunger and stress: the role of rpoS in early stationary phase gene regulation in Escherichia coli . Cell 12:165–168
    [Google Scholar]
  13. Kim Y., Watrud L.S., Matin A. 1995; A carbon starvation survival gene of Pseudomonas putida is regulated by σ34 . J Bacteriol 177:1850–1859
    [Google Scholar]
  14. Klotz M.G., Anderson A.J. 1994; The role of catalase isoenzymes in the culturability of the root colonizer Pseudomonas putida after exposure to hydrogen peroxide and antibiotics. Can J Microbiol 40:382–387
    [Google Scholar]
  15. Mannervik B., Danielson H. 1988; Glutathione transferase-structure and catalytic activity. CRC Crit Rev Biochem 23:283–337
    [Google Scholar]
  16. Matin A. 1992; Physiology, molecular biology and applications of the bacterial starvation response. J Appl Bacteriol 73: (Symp Suppl) 495–575
    [Google Scholar]
  17. Meury J., Kepes A. 1982; Glutathione and the gated potassium channels of Escherichia coli . EMBO J 1:339–343
    [Google Scholar]
  18. Murata K., Inoue Y., Rhee H., Kimura A. 1989; 2-Oxoaldehyde metabolism in microorganisms. Can J Microbiol 35:423–431
    [Google Scholar]
  19. Rhee H., Murata K., Kimura A. 1987; Metabolism of 2-ketoaldehydes in bacteria: oxidative conversion of methylglyoxal to pyruvate by an enzyme from Pseudomonas putida . Agric Biol Chem 51:1059–1066
    [Google Scholar]
  20. Rowland G.C., Giffard P.M., Booth I.R. 1984; Genetic studies of the phs locus of Escherichia coli, a mutation causing pleiotropic lesions in metabolism and pH homeostasis. FEBS Lett 173:295–300
    [Google Scholar]
  21. Summer K., Goggelmann W. 1980; Mutagenicity of 1-fluoro-2,4-dinitrobenzene is affected by bacterial glutathione. Mutat Res 70:173–178
    [Google Scholar]
  22. Zablotowicz R. M., Hoagland R. E., Locke M. A., Hickey W. J. 1995; Glutathione-S-transferase activity and metabolism of glutathione conjugates by rhizosphere bacteria. Appl Environ Microbiol 61:1054–1060
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/13500872-142-10-2857
Loading
/content/journal/micro/10.1099/13500872-142-10-2857
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error