1887

Abstract

A cluster of arginine biosynthetic genes of ATCC 13032, comprising and as well as part of and has been cloned by heterologous complementation of an mutant. The gene order has been established as by sequencing the entire 4.4 kb cloned DNA fragment. The gene can be transcribed in cells from an internal promoter located in the coding part of the preceding gene, whereas transcription of the gene appears vector-dependent. Expression of the corynebacterial gene is repressed by arginine in the native host but not in recombinant cells. Feedback inhibition of the corresponding -acetylglutamate kinase activity was observed both in cell extracts of and in recombinant auxotrophic strains. Extracts of cells carrying cloned corynebacterial DNA display an ornithine acetyltransferase activity (encoded by which alleviates the acetylornithinase (encoded by deficiency of the enterobacterial host. In contrast to ornithine acetyltransferase which also exhibits acetylglutamate synthase activity, ornithine acetyltransferase appears monofunctional. ArgA and ArgB proteins from different sources share highly significant similarities. The evolutionary implications of these data are discussed.

Loading

Article metrics loading...

/content/journal/micro/10.1099/13500872-142-1-99
1996-01-01
2021-03-04
Loading full text...

Full text loading...

/deliver/fulltext/micro/142/1/mic-142-1-99.html?itemId=/content/journal/micro/10.1099/13500872-142-1-99&mimeType=html&fmt=ahah

References

  1. An G., Friesen J. D. 1979; Plasmid vehicles for direct cloning of Escherichia coli promoters. J Bacterial 140:400–407
    [Google Scholar]
  2. Birnboim H. C., Doly J. 1979; A rapid alkaline extraction procedure for screening recombinant DNA. Nucleic Acids Res 7:1513–1523
    [Google Scholar]
  3. Bolivar F. 1978; Construction and characterization of new cloning vehicles. III. Derivatives of plasmid pBR322 carrying unique EcoRI sites for selection of EcoRI generated recombinant DNA molecules. Gene 4:121–130
    [Google Scholar]
  4. Boonchird C., Messengey F., Dubois S. 1991; Characterization of the yeast ARG5,6 gene: determination of the nucleotide sequence, analysis of the control region and of ARG5,6 transcript. Mol & Gen Genet 226:154–166
    [Google Scholar]
  5. Bróer S., Eggeling L., Kramer R. 1993; Strains of Corynebacterium glutamicum with different lysine productivities may have different lysine excretion systems. Appl Environ Microbiol 59:316–321
    [Google Scholar]
  6. Brown K., Finch P. W., Hickson I. D., Emmerson P. T. 1987; Complete nucleotide sequence of the Escherichia coli argA gene. Nucleic Acids Res 15:10586
    [Google Scholar]
  7. Cunin R., Glansdorff N., Pierard A., Stalon V. 1986; Biosynthesis and metabolism of arginine in bacteria. Microbiol Rev 50:314–352
    [Google Scholar]
  8. Davis R. H. 1986; Compartmental and regulatory mechanisms in the arginine pathways of Neurospora crassa and Saccharomyces cerevisiae. Microbiol Rev 50:280–313
    [Google Scholar]
  9. Dharmsthiti S., Krishnapillai V. 1993; DNA sequence conservation at the gene level in a conserved chromosomal segment in two Pseudomonas species. J Genet 72:1–14
    [Google Scholar]
  10. Eikmanns B. J., Kircher M., Liebl W., Reinscheid D. J. 1991; Discrimination of Corynebacterium glutamicum, Brevibacterium flavum and Brevibacterium lactojermentum by restriction pattern analysis of DNA adjacent to the horn gene. FEMS Microbiol Lett 82:203–208
    [Google Scholar]
  11. Gessert S. F., Kim J. H., Nargang F. E., Weiss R. L. 1994; A polyprotein precursor of two mitochondrial enzymes in Neurospora crassa. Gene structure and precursor processing. J Biol Chem 269:8189–8203
    [Google Scholar]
  12. Haas D., Leisinger T. 1974; Multiple control of IV-acetylglutamate synthetase from Pseudomonas aeruginosa: synergistic inhibition by acetylglutamate and polyamines. Biochem Biophys Res Commun 60:42–47
    [Google Scholar]
  13. Haas D., Leisinger T. 1975; N-acetylglutamate 5-phosphotransferase of Pseudomonas aeruginosa. Catalytic and regulatory properties. Eur J Biochem 52:377–383
    [Google Scholar]
  14. Haas D., Kurer V., Leisinger T. 1972; N-acetylglutamate synthetase of Pseudomonas aeruginosa. An assay in vitro and feedback inhibition by arginine. Eur J Biochem 31:290–295
    [Google Scholar]
  15. Higgins D. G., Sharp P. M. 1988; clustal: a package for performing multiple sequence alignment on a microcomputer. Gene 73:237–244
    [Google Scholar]
  16. Hindle Z., Callis R., Dowden S., Rudd B. A. M., Baumberg S. 1994; Cloning and expression in Escherichia coli of a Streptomyces coelicolor A3(2) argCJB gene cluster. Microbiology 140:311–320
    [Google Scholar]
  17. Hoare D. S., Hoare S. L. 1966; Feedback regulation of arginine biosynthesis in blue-green algae and photosynthetic bacteria. J Bacteriol 92:375–379
    [Google Scholar]
  18. Horinouchi S., Furuya K., Nishiyama M., Suzuki H., Beppu T. 1987; Nucleotide sequence of the streptothrycin acetyltransferase gene from Streptomyces lavendulae and its expression in heterologous hosts. J Bacteriol 169:1929–1937
    [Google Scholar]
  19. Jones D., Collins M. D. 1986; Irregular, nonsporing Gram-positive rods. Bergey's Manual of Systematic Bacteriology 21261–1434 Edited by Sneath P. H. A., Mair N. S., Sharpe M. E., Holt J. G. Baltimore: Williams & Wilkins;
    [Google Scholar]
  20. Kinoshita S., Nakayama K. 1978; Amino acids. Primary Products of Metabolism209–261 Edited by Rose A. H. London, New York & San Francisco: Academic Press;
    [Google Scholar]
  21. Makino K., Kim S., Shinagawa H., Amemura M., Nataka A. 1991; Molecular analysis of the cryptic and functional phn operons for phosphonate use in Escherichia coli K-12. J Bacteriol 173:2665–2672
    [Google Scholar]
  22. Malumbres M., Gil J. A., Martin J. F. 1993; Codon preference in Corynebacteria. Gene 134:15–24
    [Google Scholar]
  23. Martin P. R., Mulks M. H. 1992; Sequence analysis and complementation studies of the argJgene encoding ornithine acetyltransferase from Neisseria gonorrhoeae. J Bacteriol 174:2694–2701
    [Google Scholar]
  24. Meile L., Leisinger T. 1984; Enzymes of arginine biosynthesis in methanogenic bacteria. Experientia 40:899–900
    [Google Scholar]
  25. Miller J. H. 1972 Experiments in Molecular Genetics Cold Spring Harbor, NY: Cold Spring Harbor Laboratory;
    [Google Scholar]
  26. Mountain A., Mann N. H., Munton R. N., Baumberg S. 1984; Cloning of a Bacillus subtilis restriction fragment complementing auxotrophic mutants of eight Escherichia coli genes of arginine biosynthesis. Mol & Gen Genet 19782–89
    [Google Scholar]
  27. Mountain A., McChesney J., Smith M. C. M., Baumberg S. 1986; Gene sequence encoding early enzymes of arginine synthesis within a cluster in Bacillus subtilis, as revealed by cloning in Escherichia coli. J Bacteriol 165:1026–1028
    [Google Scholar]
  28. O'Reilly M., Devine K. M. 1994; Sequence and analysis of the citrulline biosynthetic operon argC-F from Bacillus subtilis. Microbiology 140:1023–1025
    [Google Scholar]
  29. Parsot C., Boyen A., Cohen G. N., Glansdorff N. 1988; Nucleotide sequence of Escherichia coli argB and argC genes: comparison of N-acetylglutamate kinase and N-acetylglutamate-)'-semialdehyde dehydrogenase with homologous and analogous enzymes. Gene 68:275–283
    [Google Scholar]
  30. Pearson W. R., Lipman D. J. 1988; Improved tools for biological sequence comparison. Proc Natl Acad Sci USA 85:2444–2448
    [Google Scholar]
  31. Picard F. J., Dillon J. R. 1989; Cloning and organization of seven arginine biosynthesis genes from Neisseria gonorrhoeae. J Bacteriol 171:1644–1651
    [Google Scholar]
  32. Reith M., Munholland J. 1993; Two amino-acid biosynthetic genes are encoded on the plasmid genome of the red alga Porphyra umbilicalis. Curr Genet 23:59–65
    [Google Scholar]
  33. Sakanyan V. A., Hovsepyan A. S., Mett I.L., Kochikyan A. V., Petrosyan P. K. 1990; Molecular cloning and structural-functional analysis of the arginine biosynthesis genes of the thermophilic bacterium Bacillus stearothermophilus. Genetika 26:1915–1925
    [Google Scholar]
  34. Sakanyan V., Kochikyan A., Mett I., Legrain C., Charlier D., Piéard A., Glansdorff N. 1992; A re-examination of the pathway for ornithine biosynthesis in a thermophilic and two mesophilic Bacillus species. J Gen Microbiol 138:125–130
    [Google Scholar]
  35. Sakanyan V., Charlier D., Legrain C., Kochikyan A., Mett I., Piéard A., Glansdorff N. 1993a; Primary structure, partial purification and regulation of key enzymes of the acetyl cycle of arginine biosynthesis in Bacillus stearothermophilus: dual function of ornithine acetyltransferase. J Gen Microbiol 139:393–402
    [Google Scholar]
  36. Sakanyan V. A., Legrain C., Charlier D., Kochikyan A. V., Osina N. K., Glansdorff N. 1993b; N-acetylglutamate-5-phospho-transferase of the thermophilic bacterium Bacillus stearothermophilus: nucleotide sequence and enzyme characterization. Genetika 29:556–570
    [Google Scholar]
  37. Sakanyan V., Desmarez L., Legrain C., Charlier D., Mett I., Kochikyan A., Savchenko A., Boyen A., Falmagne P., Piéard A., Glansdorff N. 1993c; Gene cloning, sequence analysis, purification, and characterization of a thermostable aminoacylase from Bacillus stearothermophilus. Appl Environ Microbiol 59:3878–3888
    [Google Scholar]
  38. Sambrook J., Fritsch E. F., Maniatis T. 1989 Molecular Cloning: a Laboratory Manual, 2nd edn.. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory;
    [Google Scholar]
  39. Sanger F., Nicklen S., Coulson A. R. 1977; DNA sequencing with chain-terminating inhibitors. Proc Natl Acad Sci USA 74:5463–5467
    [Google Scholar]
  40. Shiio I., Otsuka S. I., Takahashi M. 1962; Effect of biotin on the bacterial formation of glutamic acid. I. Glutamate formation and cellular permeability of amino acids. J Biochem 51:56–62
    [Google Scholar]
  41. Shinners E. N., Catlin B. W. 1978; Arginine biosynthesis in Neisseria gonorrhoeae: enzymes catalyzing the formation of ornithine and citrulline. J Bacteriol 136:131–135
    [Google Scholar]
  42. Udaka S. 1966; Pathway-specific pattern of control of arginine biosynthesis in bacteria. J Bacteriol 91:617–621
    [Google Scholar]
  43. Udaka S., Kinoshita S. 1958; Studies on l-ornithine fermentation. I. The biosynthetic pathway of l-ornithine in Micrococcus glutamicus. J Gen Appl Microbiol 4:272–282
    [Google Scholar]
  44. Van de Casteele M., Demarez M., Legrain C., Glansdorff N., Pterard A. 1990; Pathways of arginine biosynthesis in extreme thermophilic archaeo- and eubacteria. J Gen Microbiol 136:1177–1183
    [Google Scholar]
  45. Van Huffel C., Dubois E., Messenguy F. 1992; Cloning and sequencing of arg3 and argil genes of Schizosaccharomycespombe on a 10-kb DNA fragment. Heterologous expression and mitochondrial targeting of their translation products. Eur J Biochem 205:33–43
    [Google Scholar]
  46. Vieira J., Messing J. 1982; The pUC plasmids, a M13mp7-derived system for insertion mutagenesis and sequencing with synthetic universal primers. Gene 19:259–268
    [Google Scholar]
  47. Vogel H. J., MacLellan W. L. 1970; Acetylornithinase (E. coli). Methods Enzymol 17A:265–269
    [Google Scholar]
  48. Wipf B., Leisinger T. 1979; Regulation of activity and synthesis of N-acetylglutamate synthase from Saccharomjces cerevisiae. J Bacteriol 140:874–880
    [Google Scholar]
  49. Yamada K., Komagata K. 1970; Taxonomic studies on coryneform bacteria. III. DNA base composition of coryneform bacteria. J Gen Appl Microbiol 16:55–65
    [Google Scholar]
  50. Yanisch-Perron C., Vieira J., Messing J. 1985; Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13mpl8 and pUC19 vectors. Gene 33:103–119
    [Google Scholar]
  51. Yoshikawa A., Isono S., Sheback A., Isono K. 1987; Cloning and nucleotide sequencing of the genes riml and rimJ which encode enzymes acetylating ribosomal proteins SI 8 and S5 of Escherichia coli. Mol & Gen Genet 209:481–488
    [Google Scholar]
  52. Zimmer W., Aparicio C., Elmerich C. 1991; Relationship between tryptophan biosynthesis and indole-3-acetic acid production in Azospirillum: identification and sequencing of a trpGDC cluster. Mol & Gen Genet 229:41–51
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/13500872-142-1-99
Loading
/content/journal/micro/10.1099/13500872-142-1-99
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error