1887

Abstract

The concentration-dependence of the inhibition of whole-cell hydrogen formation by oxygen has been measured in the trichomonads and compared with the oxygen inhibition of the hydrogenase activity as measured by a tritium exchange assay. The inhibition profiles closely paralleled each other, suggesting that hydrogenase is the primary site of inhibition of anaerobic fermentative metabolism. In addition the inhibition profile for isolated hydrogenosomes was measured and shown to be similar to that for whole organisms. Ascorbate peroxidase was shown to be present in both organisms whereas catalase was confirmed to be present only in The kinetic parameters of both enzymes were measured and their respective roles in oxygen protection discussed.

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1996-01-01
2021-08-04
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References

  1. Adams M. W. W., Mortenson L. E., Chen J.-S. 1981; Hydrogenase. Biochim Biophys Acta 594:105–176
    [Google Scholar]
  2. Boveris A., Sies H., Martino E. E., Docampo R., Turrens J. F., Stopani A. O. M. 1980; Deficient metabolic utilization of hydrogen peroxide in Trypanosoma cruzi. Biochem J 188:643–648
    [Google Scholar]
  3. Brown D. M., Upcroft J. A., Upcroft P. 1993; Cysteine is the major low-molecular weight thiol in Giardia duodenalis. Mol Biochem Parasitol 61:155–158
    [Google Scholar]
  4. Cerkasovova A., Cerkasov J., Kulda J. 1984; Metabolic differences between metronidazole resistant and susceptible strains of Tritrichomonas foetus. Mol Biochem Parasitol 11:105–118
    [Google Scholar]
  5. Daday A., Platz R. A., Smith G. D. 1977; Anaerobic and aerobic hydrogen formation by the blue-green alga Anabaena cylindrica. Appl Environ Microbiol 34:478–483
    [Google Scholar]
  6. Diamond L. S. 1955; The establishment of various trichomonads of animals and man in axenic cultures. J Parasitol 43:488–490
    [Google Scholar]
  7. Ellis J. E., Cole D., Lloyd D. 1992; Influence of oxygen on the fermentative metabolism of metronidazole-sensitive and resistant strains of Trichomonas vaginalis. Mol Biochem Parasitol 56:79–88
    [Google Scholar]
  8. Ewart G. D., Smith G. D. 1989; Purification and properties of soluble hydrogenase from the cyanobacterium Anabaena cylindrica. Arch Biochem Biophys 268:327–337
    [Google Scholar]
  9. Halliwell B., Gutteridge J. M. C. 1989 Free Radicals in Biology and Medicine, 2nd edn. Oxford: Oxford University Press;
    [Google Scholar]
  10. Hrdy I., Mertens E., Van Schaftingen E. 1993; Identification, purification and separation of different isoenzymes of NADP-specific malic enzyme from Tritrichomonas foetus. Mol Biochem Parasitol 57:253–260
    [Google Scholar]
  11. Lindmark D. G., Müller M. 1973; Hydrogenosome, a cytoplasmic organelle of the anaerobic flagellate, Tritrichomonas foetus, and its role in pyruvate metabolism. J Biol Chem 248:7724–7728
    [Google Scholar]
  12. Lindmark D. G., Müller M., Shio H. 1975; Hydrogenosomes in Trichomonas vaginalis. J Parasitol 61:552–554
    [Google Scholar]
  13. Lloyd D., Kristensen B. 1985; Metronidazole inhibition of hydrogen production in vivo in drug-sensitive and resistant strains of Trichomonas vaginalis. J Gen Microbiol 131:849–853
    [Google Scholar]
  14. Miyake C, Michihata F., Asada K. 1991; Scavenging of hydrogen peroxide in prokaryotic and eukaryotic algae: acquisition of ascorbate peroxidase during the evolution of cyanobacteria. Plant Cell Physiol 32:33–43
    [Google Scholar]
  15. Müller M. 1973; Biochemical cytology of trichomonad flagellates. I. Subcellular localization of hydrolases, hydrogenase and catalase in Tritrichomonas foetus. J Cell Biol 57:453–474
    [Google Scholar]
  16. Müller M. 1990; Biochemistry of Trichomonas vaginalis. Trichomonads Parasitic in Humans53–83 Edited by Honigberg B. M. New York: Springer-Verlag;
    [Google Scholar]
  17. Nakano Y., Asada K. 1981; Hydrogen peroxide is scavenged by ascorbate-specific peroxidase in spinach chloroplasts. Plant Cell Physiol 22:867–880
    [Google Scholar]
  18. Ninomiya H., Suzuoki-Ziro. 1952; The metabolism of Trichomonas vaginalis, with comparative aspects of trichomonads. J Biochem 39:321–331
    [Google Scholar]
  19. Pow T., Krasna A. I. 1979; Photoproduction of hydrogen from water in hydrogenase-containing algae. Arch Biochem Biophys 194:413–421
    [Google Scholar]
  20. Rio L. A. D., Ortega M. G., Lopez A. L., George J. L. 1977; A more sensitive modification of the catalase assay with the Clark oxygen electrode. Anal Biochem 80:409–415
    [Google Scholar]
  21. Ryley J. F. 1955; Studies on the metabolism of the protozoa. 5. Metabolism of the parasitic flagellate Trichomonas foetus. Biochem J 59:361–369
    [Google Scholar]
  22. Steinbuchel A., Müller M. 1986; Glycerol, a metabolic end product of Trichomonas vaginalis and Tritrichomonas foetus. Mol Biochem Parasitol 20:45–55
    [Google Scholar]
  23. Wagner G., Levin R. 1978; Oxygen tension of the vaginal surface during sexual stimulation in the human. Fertil Steril 30:50–53
    [Google Scholar]
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