1887

Abstract

A protocol was developed for the introduction of foreign plasmid DNA into various strains. The method, which is applicable to all species tested so far, is based on electroporation of bacteria made competent by preincubation in electroporation buffer for several hours at 4 °C. Transformation of could be achieved with a plasmid vector originating from and with plasmid vectors from but not with vectors carrying replicons from or

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1996-01-01
2021-07-26
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