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Five new mutants of Neurospora crassa that require supplementation with unsaturated fatty acids have been isolated. The mutants, designated pfa, are impaired in the synthesis of the polyunsaturated fatty acids α-linoleic or α-linolenic acid, but are able to synthesize oleic acid. The pfa mutants are thus distinct from previously described ufa mutants, which are unable to synthesize oleic acid. The five pfa mutants map to distinct loci, and have characteristic patterns of incorporation of [14C]acetate and [14C]oleate into their fatty acids.
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