1887

Abstract

Copper- and zinc-containing superoxide dismutases ([Cu,Zn]-SODs) are generally considered almost exclusively eukaryotic enzymes, protecting the cytosol and extracellular compartments of higher organisms from damage by oxygen free-radicals. The recent description of a few examples of bacterial forms of the enzyme, located in the periplasm of different Gram-negative micro-organisms, prompted a re-evaluation of this general perception. A PCR-based approach has been developed and used successfully to identify bacterial genes encoding [Cu,Zn]-SOD in a wide range of important human and animal pathogens - members of the and (HAP) group, and Comparison of [Cu,Zn]-SOD peptide sequences found in and with previously described bacterial proteins and examples of eukaryotic [Cu,Zn]-SOD has shown that the bacterial proteins constitute a distinct family apparently widely separated in evolutionary terms from the eukaryotic examples. The widespread occurrence of [Cu,Zn]-SOD in the periplasm of bacterial pathogens, appropriately located to dismute exogenously derived superoxide radical anions, suggests that this enzyme may play a role in the interactive biology of organisms with their hosts and so contribute to their capacity to cause disease.

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1995-09-01
2021-08-04
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References

  1. Bannister J.V., Parker M.W. 1985; The presence of a copper- zinc superoxide dismutase EC 1.15.1.1 in the bacterium Photo-bacterium leiognathi-, a likely case of gene transfer from eukaryotes to prokaryotes.. Proc Natl Acad Sci USA 82:149–152
    [Google Scholar]
  2. Barra D., Martini F., Bannister J.V., Schinina M.E., Rotilio G., Bannister W.H., Bosa F. 1980; The complete amino acid sequence of human Cu/Zn superoxide dismutase.. FEBS Lett 120:53–56
    [Google Scholar]
  3. Beaman L., Beaman B.L. 1990; Monoclonal antibodies demonstrate that superoxide dismutase contributes to protection of Nocardia asteroides within the intact host.. InfectImmun 58:3122–3128
    [Google Scholar]
  4. Beauchamp C.O., Fridovich I. 1971; Superoxide dismutase: improved assays and an assay applicable to acrylamide gels.. Anal Biochem 72:276–287
    [Google Scholar]
  5. Beck B., Tabatabai L.B., Mayfield J.E. 1990; A protein isolated from Brucella abortus is a Cu-Zn superoxide dismutase.. Biochemistry 29:372–376
    [Google Scholar]
  6. Benov L.T., Fridovich I. 1994; Escherichia coli expresses a copper- and zinc-containing superoxide dismutase.. J Biol Chem 269:25310–25314
    [Google Scholar]
  7. Bordo D., Djinovic K., Bolognesi M. 1994; Conserved patterns in the Cu, Zn superoxide dismutase family.. J Mol Biol 238:366–386
    [Google Scholar]
  8. Davis B.J. 1964; Disc electrophoresis. II. Method and applications to human serum proteins.. Ann NY Acad Sci USA 121:404–427
    [Google Scholar]
  9. Flohe L. 1984; The phylogenetic position of the copper/zinc superoxide dismutase of Photobacterium leiognathi.. In Oxygen Radicals in Chemistry and Biology pp. 793–799 Bors W., Saran M., Tait D. Edited by Berlin: Walter de Gruyter;
    [Google Scholar]
  10. Getzoff E.D., Tainer J.A., Stempien M.M., Bell G.I., Hallewell R.A. 1989; Evolution of CuZn superoxide dismutase and the Greek key beta-barrel structural motif.. Proteins 5:322–336
    [Google Scholar]
  11. Gonnet G.H. 1992; A tutorial introduction to Computational Biochemistry using DARWIN. Zurich: Informatik E.T.H. (Obtainable from the author at Institut för Wissenschaftliches Rechnen, ETH-Zentrum, CH-8092, Zurich.).
    [Google Scholar]
  12. Gonnet G.H. 1994; New algorithms for the computation of evolutionary phylogenetic trees.. In Computational Methods in Genome Research pp. 153–161 Suhai S. Edited by New York: Academic Press;
    [Google Scholar]
  13. Gonnet G.H., Cohen M.A., Benner S.A. 1992; Exhaustive matching of the entire protein sequence database.. Science 256:1443–1445
    [Google Scholar]
  14. Grace S.C. 1990; Phylogenetic distribution of superoxide dismutase supports an endosymbiotic origin for chloroplasts and mitochondria.. Life Sci 47:1875–1886
    [Google Scholar]
  15. Hanahan D. 1983; Studies on transformation of Escherichia coli with plasmids.. J Mol Biol 166:557–580
    [Google Scholar]
  16. Hassan H.M., Fridovich I. 1979; Paraquat and Escherichia coli.. J Biol Chem 254:10846–10852
    [Google Scholar]
  17. Henkle K.J., Liebau E., Muller S., Bergmann B., Walter R.D. 1991; Characterization and molecular cloning of a Cu/Zn superoxide dismutase from the human parasite Onchocerca volvulus.. Infect Immun 59:2063–2069
    [Google Scholar]
  18. Hettari M., Sakakai Y. 1986; Dideoxy sequencing method using denatured plasmid templates.. Anal Biochem 152:232–238
    [Google Scholar]
  19. Hjalmarsson K., Marklund S.L., Engstrom A., Edlund T. 1987; Isolation and sequence of complementary DNA encoding human extracellular superoxide dismutase.. Proc Natl Acad Sci USA 84:6340–6344
    [Google Scholar]
  20. James E.R., McLean D.C. Jr Perler F. 1994; Molecular cloning of an Onchocerca volvulus extracellular Cu-Zn superoxide dismutase.. Infect Immun 62:713–716
    [Google Scholar]
  21. Johansen T.T., Overballe-Petersen C., Martin B., Hasemann V., Svendsen I. 1979; The complete amino acid sequence of copper, zinc superoxide dismutase from Saccharomyces cerevisiae.. Carlsberg Res Commun 44:201–217
    [Google Scholar]
  22. Kilian M., Nicolet J., Biberstein E.L. 1978; Biochemical and serological characterization of Haemophilus pleuropneumoniae (Matthews and Pattison 1961) Shope 1964 and proposal of a neotype strain.. Int J Syst Bacteriol 28:20–26
    [Google Scholar]
  23. Kroll J.S., Loynds B., Brophy L.N., Moxon E.R. 1990; Thebex locus in encapsulated Haemophilus influenzae-, a chromosomal region involved in capsule polysaccharide export.. Mol Microbiol 4:1853–1862
    [Google Scholar]
  24. Kroll J.S., Langford P.R., Loynds B.M. 1991; Copper-zinc superoxide dismutase of Haemophilus influenzae and H.parainfluenzye.. J Bacteriol 173:7449–7457
    [Google Scholar]
  25. Langford P.R., Loynds B.M., Kroll J.S. 1992; Copper-zinc superoxide dismutase in Haemophilus species.. J Gen Microbiol 138:517–522
    [Google Scholar]
  26. McCord J.M., Fridovich I. 1969; Superoxide dismutase. An enzymatic function for erythrocuprein (hemocuprein).. J Biol Chem 244:6049–6055
    [Google Scholar]
  27. McGuinness B.T., Clarke I.N., Lambden P.R., Barlow A.K., Poolman J.T., Jones D.M., Heckels J.E. 1991; Point mutation in meningococcal porA gene associated with increased endemic disease.. Lancet 337:514–516
    [Google Scholar]
  28. Norrod P., Morse S.A. 1979; Absence of superoxide dismutase in some strains of Neisseria gonorrhoeae.. Biochem Biophys Res Commun 90:1287–1294
    [Google Scholar]
  29. Puget K., Michelson A.M. 1974; Isolation of a new copper- containing superoxide dismutase bacteriocuprein.. Biochem Biophys Res Commun 58:830–838
    [Google Scholar]
  30. Purcell B.K., Richardson J.A., Radolf J.D., Hansen E.J. 1991; A temperature-dependent rabbit model for production of dermal lesions by Haemophilus ducreyi.. J Infect Dis 164:359–367
    [Google Scholar]
  31. Quentin R., Goudeau A., Wallace R.J. Jr Smith A.L., Selander R.K., Musser J.M. 1990; Urogenital, maternal and neonatal isolates of Haemophilus influenzae: identification of unusually virulent serologically non-typable clone families and evidence for a new Haemophilus species.. J Gen Microbiol 136:1203–1209
    [Google Scholar]
  32. Sambrook J., Fritsch E.F., Maniatis T. 1989 Molecular Cloning: A Laboratory Manual. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory;
    [Google Scholar]
  33. Sanger F., Nicklen S., Coulson A.R. 1977; DNA sequencing with chain-terminating inhibitors.. Proc Natl Acad Sci USA 74:5463–5467
    [Google Scholar]
  34. Sarkar G., Sommer S.S. 1990; Shedding light on PCR contamination.. Nature 343:27
    [Google Scholar]
  35. Simurda M.C., van Keulen H., Rekosh D.M., LoVerde P.T. 1988; Schistosoma mansoni-. identification and analysis of an mRNA and a gene encoding superoxide dismutase (Cu/Zn).. Exp Parasitol 67:73–84
    [Google Scholar]
  36. Stabel T.J., Sha Z., Mayfield J.E. 1994; Periplasmic location of Brucella abortus Cu/Zn superoxide dismutase.. Vet Microbiol 38:307–314
    [Google Scholar]
  37. Steinman H.M. 1982; Copper-zinc superoxide dismutase from Caulobacter crescentus CB15.. J Biol Chem 257:10283–10293
    [Google Scholar]
  38. Steinman H.M. 1985; Bacteriocuprein superoxide dismutases in Pseudomonads.. J Bacteriol 162:1255–1260
    [Google Scholar]
  39. Steinman H.M. 1987; Bacteriocuprein superoxide dismutase of Photobacterium leiognathi.. J Biol Chem 262:1882–1887
    [Google Scholar]
  40. Steinman H.M. 1992; Construction of an Escherichia coli K-12 strain deleted for manganese and iron superoxide dismutase genes and its use in cloning the iron superoxide dismutase gene of Eegionella pneumophila.. Mol & Gen Genet 232:427–430
    [Google Scholar]
  41. Steinman H.M. 1993; Function of periplasmic copper-zinc superoxide dismutase in Caulobacter crescentus.. J Bacteriol 175:1198–1202
    [Google Scholar]
  42. Steinman H.M., Ely B. 1990; Copper-zinc superoxide dismutase of Caulobacter crescentus-. cloning, sequencing, and mapping of the gene and periplasmic location of the enzyme.. J Bacteriol 172:2901–2910
    [Google Scholar]
  43. Steinman H.M., Naik V.R., Abernethy J.L., Hill R.L. 1974; Bovine erythrocyte superoxide dismutase. Complete amino acid sequence.. J Biol Chem 249:7326–7338
    [Google Scholar]
  44. Tatum F.M., Detilleux P.G., Sacks J.M., Hailing S.M. 1992; Construction of Cu,Zn superoxide dismutase deletion mutants of Brucella abortus: analysis of survival in vitro in epithelial and phagocytic cells and in vivo in mice.. Infect Immun 60:2863–2869
    [Google Scholar]
  45. Tsai C.-C., McArthur W.P., Baehni P.C., Hammond B.F., Taichman N.S. 1979; Extraction and partial characterization of a leukotoxin from a plaque-derived gram-negative microorganism.. Infect Immun 25:427–439
    [Google Scholar]
  46. Vignais P.M., Terech A., Meyer C.M., Henry M.-F. 1982; Isolation and characterization of a protein with cyanide-sensitive superoxide dismutase activity from the prokaryote Paracoccus denitrificans.. Biochim Biophys Acta 701:305–317
    [Google Scholar]
  47. Zhang Y., Lathigra R., Garbe T., Catty D., Young D. 1991; Cloning and characterisation of the superoxide dismutase gene from Mycobacterium tuberculosis.. Mol Microbiol 5:381–391
    [Google Scholar]
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