The ability of the bacteriophage 434 operator/repressor system to function in a eukaryotic cell has been explored. An idealized 434 operator was placed at various positions in the promoter of : within the upstream activator sequence, close to the TATA box, and downstream of the transcription-initiation site. Expression of the 434 gene from a 2 μm-based plasmid resulted in significant repression of gene expression from constructs containing the altered promoters linked to a β-galactosidase reporter gene. Attempts to use a variant of the 434 repressor that has the binding specificity of the P22 repressor (434) were unsuccessful, due to a severely inhibitory effect of this gene-product on the growth of the yeast cells.


Article metrics loading...

Loading full text...

Full text loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error