1887

Abstract

The ability of the bacteriophage 434 operator/repressor system to function in a eukaryotic cell has been explored. An idealized 434 operator was placed at various positions in the promoter of : within the upstream activator sequence, close to the TATA box, and downstream of the transcription-initiation site. Expression of the 434 gene from a 2 μm-based plasmid resulted in significant repression of gene expression from constructs containing the altered promoters linked to a β-galactosidase reporter gene. Attempts to use a variant of the 434 repressor that has the binding specificity of the P22 repressor (434) were unsuccessful, due to a severely inhibitory effect of this gene-product on the growth of the yeast cells.

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1995-09-01
2021-10-22
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References

  1. Aggarwal A.K., Rodgers D.W., Drotar M., Ptashne M., Harrison S.C. 1988; Recognition of a DNA operator by the repressor of phage 434: a view at high resolution.. Science 242:899–907
    [Google Scholar]
  2. Anderson J.E., Ptashne M., Harrison S.C. 1985; A phage repressor operator at 7 Å resolution.. Nature 316:596–601
    [Google Scholar]
  3. Anderson J.E., Ptashne M., Harrison S.C. 1987; Structure of the repressor-operator complex of bacteriophage 434.. Nature 326:846–852
    [Google Scholar]
  4. Beier D.R., Young E.T. 1982; Characterisation of a regulatory region upstream of the ARD2 locus of S.cerevisiae. . Nature 300:724–728
    [Google Scholar]
  5. Brent R., Ptashne M. 1984; A bacterial repressor protein or a yeast transcriptional terminator can block upstream activation of a yeast gene.. Nature 312:612–615
    [Google Scholar]
  6. Brown M., Figge J., Hansen U., Wright C., Jeang K.-T., Khoury G., Livingston D.M., Roberts T.M. 1987; lac repressor can regulate expression from a hybrid SV40 early promoter containing a lac operator in animal cells.. Cell 49:603–612
    [Google Scholar]
  7. Bullock W.O., Fernandez J.M., Short J.M. 1987; XLl-blue: a high efficiency plasmid transforming recA Escherichia coli strain with beta-galactosidase selection.. Biotechniques 5:376–379
    [Google Scholar]
  8. Chambers A., Stanway C., Kingsman A.J., Kingsman S.M. 1988; The UAS of the yeast PGK gene is composed of multiple functional elements.. Nucleic Acids Res 16:8245–8260
    [Google Scholar]
  9. Chambers A., Tsang J.S.H., Stanway C., Kingsman A.J., Kingsman S.M. 1989; Transcriptional control of the Saccharomjces cerevisiae PGK gene by RAPl.. Mol Cell Biol 9:5516–5526
    [Google Scholar]
  10. Chambers A., Stanway C., Tsang J.S.H., Henry Y., Kingsman A.J., Kingsman S.M. 1990; ARS binding factor 1 binds adjacent to RAPl at the UASs of the yeast glycolytic genes PGK and PYK1. . Nucleic Acids Res 18:5393–5399
    [Google Scholar]
  11. Derynck R., Singh A., Goeddel D.V. 1983; Expression of the human interferon-Ɣ cDNA in yeast.. Nucleic Acids Res 11:1819–1837
    [Google Scholar]
  12. Devereaux J., Haeberli P., Smithies O. 1984; A comprehensive set of sequence analysis programs for the VAX.. Nucleic Acids Res 12:387–395
    [Google Scholar]
  13. Deuschle U., Hipskind R.A., Bujard H. 1990; RNA polymerase II transcription blocked by Escherichia coli lac repressor.. Science 248:480–483
    [Google Scholar]
  14. Dingermann T., Frank-Stoll U., Werner H., Wissmann A., Hillen W., Jacquet M., Marschalek R. 1992; RNA polymerase III catalysed transcription can be regulated in Saccharomyces cerevisiae by the bacterial tetracycline repressor-operator system.. EMBO J 11:1487–1492
    [Google Scholar]
  15. Figge J., Wright C., Collins C.J., Roberts T.J., Livingston D.M. 1988; Stringent regulation of stably integrated chloramphenicol acetyl transferase genes by E. coli lac repressor in monkey cells.. Cell 52:713–728
    [Google Scholar]
  16. Gatz C., Quail P.H. 1988; Tn10-encoded tet repressor can regulate an operator-containing plant promoter.. Proc Natl Acad Sci USA 851394–1397
    [Google Scholar]
  17. Gatz G, Kaiser A., Wendenburg R. 1991; Regulation of a modified CaMV 35S promoter by the Tn10-encoded tet repressor in transgenic tobacco.. Mol & Gen Genet 227:229–237
    [Google Scholar]
  18. van Gorcom R.F.M., Punt P.J., Pouwels P.H., van den Hondel C. A. M. J.J. 1986; A system for the analysis of expression signals in Aspergillus. . Gene 48:211–217
    [Google Scholar]
  19. Hadfield C., Cashmore A., Meacock P.A. 1986; An efficient chloramphenicol resistance marker for Saccharomyces cerevisiae and Escherichia coli. . Gene 45:149–158
    [Google Scholar]
  20. Hadfield C., Cashmore A.M., Meacock P.A. 1987; Sequence and expression characteristics of a shuttle chloramphenicol-resistance marker for Saccharomyces cerevisiae and Escherichia coli. . Gene 52:59–70
    [Google Scholar]
  21. Hadfield G, Jordan B.E., Mount R.C., Pretorious G.H.S., Burak E. 1990; G418 resistance as a dominant marker and reporter for gene expression in Saccharomyces cerevisiae. . Curr Genet 18:303–313
    [Google Scholar]
  22. Hanahan D. 1983; Studies on transformation of Escherichia coli with plasmids.. J Mol Biol 166:557–580
    [Google Scholar]
  23. Hollis M., Vakenzuela D., Pioli D., Wharton R., Ptashne M. 1988; A repressor heterodimer binds to a chimeric operator.. Proc Natl Acad Sci USA 855834–5838
    [Google Scholar]
  24. Hu M.C.-T., Davidson N. 1987; The inducible lac operatorrepressor system is functional in mammalian cells.. Cell 48:555–566
    [Google Scholar]
  25. Johnston M., Davis R.W. 1984; Sequences that regulate the divergent GAL1-GAL10 promoter in Saccharomyces cerevisiae. . Mol Cell Biol 4:1440–1448
    [Google Scholar]
  26. Koudelka G.B., Harburg P., Harrison S.C., Ptashne M. 1988; DNA twisting and affinity of bacteriophage 434 operator for bacteriophage 434 repressor.. Proc Natl Acad Sci USA 854633–4637
    [Google Scholar]
  27. Künkel T.A., Roberts J.D., Zakour R.A. 1987; Rapid and efficient site-specific mutagenesis without phenotypic selection.. Methods Enzymol 154:367–373
    [Google Scholar]
  28. Lanzer M., Bujard H. 1988; Promoters largely determine the efficiency of repressor action.. Proc Natl Acad Sci USA 858973–8977
    [Google Scholar]
  29. Marsh L., Erfle M., Wykes E.J. 1984; The pIC plasmid and phage vectors with versatile cloning sites for recombination selection by insertional inactivation.. Gene 32:481–486
    [Google Scholar]
  30. Miller J.H. Experiments in Molecular Genetics pp. 352–355 Cold Spring Harbor, NY: Cold Spring Harbor Laboratory;
    [Google Scholar]
  31. Ogden J.E., Stanway C., Kim S., Mellor J., Kingsman A.J., Kingsman S.M. 1986; Efficient expression of the Saccharomyces cerevisiae PGK gene depends on an upstream activation sequence but does not require TATA sequences.. Mol Cell Biol 6:4335–4343
    [Google Scholar]
  32. Orr-Weaver T.G, Szostak J.W., Rothstein R.J. 1981; Yeast transformation; a model system for the study of recombination.. Proc Natl Acad Sci USA 786354–6358
    [Google Scholar]
  33. Pabo C.O., Sauer R.T. 1984; Protein-DNA recognition.. Annu Rev Biochem 53:293–321
    [Google Scholar]
  34. Rathjen J., Mellor J. 1990; Characterisation of sequences required for RNA initiation from the PGK promotor of Saccharomyces cerevisiae. . Nucleic Acids Res 18:3219–3225
    [Google Scholar]
  35. Riggs A.D., Suzuki H., Bourgeois S. 1970; lacrepressor-operator interaction. I. Equilibrium studies.. J Mol Biol 48:67–83
    [Google Scholar]
  36. Sambrook J., Fritsch E.F., Maniatis T. 1989 Molecular Cloning: a Eaboratory Manual. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory;
    [Google Scholar]
  37. Sanger F., Donelson J.E., Coulson A.R., Koessel H., Fisher D. 1974; Determination of a nucleotide sequence in bacteriophage F-l DNA by primed synthesis with DNA polymerase.. J Mol Biol 90:315–333
    [Google Scholar]
  38. Smith G.M., Mileham K.A., Cooke S.E., Woolston S.J., George H.K., Charles A.D., Brammar W.J. 1988; The Escherichia coli lexA repressor-operator system works in mammalian cells.. EMBO J 7:3975–3982
    [Google Scholar]
  39. Soberon X., Covarrubias L., Bolivar F. 1980; Construction and characterisation of new cloning vehicles. 4. Deletion derivatives of pBR322 and pBR325.. Gene 9:287–305
    [Google Scholar]
  40. Stanway C., Mellor J., Ogden J.E., Kingsman A.J., Kingsman S.M. 1987; The UAS of the yeast PGK gene contains functionally distinct domains.. Nucleic Acids Res 15:6865–6873
    [Google Scholar]
  41. Stanway C., Chambers A., Kingsman A.J., Kingsman S.M. 1989; Characterisation of the transcriptional potency of subelements of the UAS of the yeast PGK gene in a PGK mini-promotor.. Nucleic Acids Res 17:9205–9218
    [Google Scholar]
  42. Strickberger M.W. 1976 Genetics p. 54 New York: Collier MacMillan;
    [Google Scholar]
  43. Webster C., Merryweather A., Brammar W. 1992; Efficient repression by a heteromeric repressor in Escherichia coli. . Mol Microbiol 6:371–377
    [Google Scholar]
  44. Wharton R.P., Ptashne M. 1985; A new specificity mutant of 434 repressor that defines an amino acid-base pair contact.. Nature 326:888–891
    [Google Scholar]
  45. Wharton R.P., Ptashne M. 1987; Changing the binding specificity of a repressor by redesigning an α-helix.. Nature 316:601–605
    [Google Scholar]
  46. Wharton R.P., Brown E.L. 1984; Substituting an α-helix switches the sequence specific interactions of a repressor.. Cell 38:361–369
    [Google Scholar]
  47. Wilde R.J., Shufflebottom D., Cooke S., Jasinska I., Merryweather A., Beri R., Brammar W.J., Bevan M. 1992; Control of gene expression in tobacco cells using a bacterial operator-repressor system.. EMBO J 11:1251–1259
    [Google Scholar]
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