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Volume 141, Issue 9

The bacteriophage 434 operator/repressor system in yeast Free

Abstract

The ability of the bacteriophage 434 operator/repressor system to function in a eukaryotic cell has been explored. An idealized 434 operator was placed at various positions in the promoter of : within the upstream activator sequence, close to the TATA box, and downstream of the transcription-initiation site. Expression of the 434 gene from a 2 μm-based plasmid resulted in significant repression of gene expression from constructs containing the altered promoters linked to a β-galactosidase reporter gene. Attempts to use a variant of the 434 repressor that has the binding specificity of the P22 repressor (434) were unsuccessful, due to a severely inhibitory effect of this gene-product on the growth of the yeast cells.

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Keyword(s): bacteriophage 434 operator/repressor , P22 repressor , regulation of gene expression and Saccharomyces cerevisiae
© Society for General Microbiology 1995
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1995-09-01
2022-12-02
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The bacteriophage 434 operator/repressor system in yeast
Microbiology 141, 2191 (1995); https://doi.org/10.1099/13500872-141-9-2191
/content/journal/micro/10.1099/13500872-141-9-2191
/content/journal/micro/10.1099/13500872-141-9-2191
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