1887

Abstract

The thioredoxin gene, , was isolated by its ability to complement an mutant which was otherwise unable to grow on minimal medium lacking glutathione. The thioredoxin also enabled the reduction by of methionine sulfoxide to methionine, as well as the reduction of insulin. When present in , the thioredoxin supported the replication of phage T7, but not the growth of phage M13. The gene was sequenced and the thioredoxin was found to be most like that of (71% identity) and (70% identity). As in the case of , the gene was located immediately upstream of the gene for the transcriptional terminator. DNA:RNA blot hybridization and primer-extension analysis of the gene in and the cloned gene in indicated that it was transcribed as an independent unit and that the major transcriptional start sites were the same in both organisms.

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1995-09-01
2021-10-18
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