IS1500, an IS3-like element from Free

Abstract

Copies of an insertion-sequence (IS)-like element were isolated from two closely related serovars of sensu stricto. Nucleotide sequence analysis of the 1236 bp element showed a characteristic IS structure with terminal imperfect inverted repeats (IRs) flanking a 1159 bp central region. This element was designated IS 1500. Four open reading frames () were found in the central ‘unique’ region of IS 1500. Similarities were detected between ORFA and ORFB and the putative transposases from members of the IS3 family of transposable elements. IS 1500 or IS 1500-like sequences were also detected in all other pathogenic leptospiral serovars, but not in the saprophytic species Differences in IS1500 copy numbers in members of the same species suggest that this element can transpose. Physical mapping of IS 1500 insertions in serovars and showed insertions were only on the large chromosomal replicon. The location of some IS1500 insertions coincides with regions of the genome that have undergone large rearrangements.

Loading

Article metrics loading...

/content/journal/micro/10.1099/13500872-141-9-2165
1995-09-01
2024-03-29
Loading full text...

Full text loading...

/deliver/fulltext/micro/141/9/mic-141-9-2165.html?itemId=/content/journal/micro/10.1099/13500872-141-9-2165&mimeType=html&fmt=ahah

References

  1. Altschul S.F., Gish W., Miller W., Myers E.W., Lipman D.J. 1990; Basic local alignment search tool.. J Mol Biol 215:403–410
    [Google Scholar]
  2. Baril C., Herrmann J.L., Richaud C., Margarita D., Saint Girons I. 1992; Scattering of the rRNA genes on the physical map of the circular chromosome of Leptospira interrogans serovar icterohaemorrhagiae.. J Bacteriol 23:7566–7571
    [Google Scholar]
  3. Birkenbihl R.P., Vielmetter W. 1990; Complete maps of IS1, IS2,IS3, IS4, IS5, IS30, and IS150 locations in Escherichia coli K12.. Mol & Gen Genet 220:147–153
    [Google Scholar]
  4. Bonamy C, Labarre J., Reyes O., Leblon G. 1994; Identification of IS 1206, a Corynebacteriumglutamicum ISJ-related insertion sequence and phylogenetic analysis.. Mol Microbiol 14:571–581
    [Google Scholar]
  5. Brennan R.G., Matthews B.W. 1989; The helix-turn-helix DNA binding motif.. J Biol Chem 264:1903–1906
    [Google Scholar]
  6. Chandler M., Fayet O. 1993; Translational frameshifting in the control of transposition in bacteria.. Mol Microbiol 7:497–503
    [Google Scholar]
  7. Chiou C.-S., Jones A.L. 1993; Nucleotide sequence analysis of a transposon (TnS393) carrying streptomycin resistance genes in Erwinia amylovora and other gram-negative bacteria.. J Bacteriol 175:732–740
    [Google Scholar]
  8. Doak T.G., Doerder F.P., Jahn C.L> , Herrick G. 1994; A proposed superfamily of transposase genes: tranposon-like elements in ciliated protozoa and a common ‘D35E’ motif.. Proc Natl Acad Sci USA 91942–946
    [Google Scholar]
  9. Ellinghausen H.C., McCullough W.G. 1965; Nutrition of Leptospira pomona and growth of 13 other serotypes: fractionation of oleic albumin complex and medium bovine albumin and polysorbate 80.. Am J Vet Res 26:45–51
    [Google Scholar]
  10. Fayet O., Ramond P., Polard P., Prere M.F., Chandler M. 1990; Functional similarities between retroviruses and the ISJ family of bacterial insertion sequences.. Mol Microbiol 4:1771–1774
    [Google Scholar]
  11. Galas D.J., Chandler M. 1989; Bacterial insertion sequences.. In Mobile DNA pp. 109–162 Edited by Berg D. E., Howe M. M. Washington, DC: American Society for Microbiology;
    [Google Scholar]
  12. Herrmann J.L, Baril C., Bellenger E., Perolat P., Baranton G., Saint Girons I. 1991; Genome conservation in isolates of Leptospira interrogans. . J Bacteriol 173:7582–7588
    [Google Scholar]
  13. Herrmann J.L, Bellenger E., Perolat P., Baranton G., Saint Girons I. 1992; Pulsed-field gel electrophoresis of Notl digests of leptospiral DNA: a new rapid method of serovar identification.. J Clin Microbiol 30:1696–1702
    [Google Scholar]
  14. Higgins D.G., Sharp P.M. 1988; clustal: a package for performing multiple sequence alignment on a microcomputer.. Gene 73:237–244
    [Google Scholar]
  15. Johnson R.C., Harris V.G. 1967; Differentiation of pathogenic and saprophytic leptospires. I. Growth at low temperatures.. J Bacteriol 94:27–31
    [Google Scholar]
  16. Kropinski A.M., Farinha M.A., Jansons I. 1994; Nucleotide sequence of the Pseudomonas aeruginosa insertion sequence IS222: another member of the IS3 family.. Plasmid 31:222–228
    [Google Scholar]
  17. Landschulz W.H., Johnson P.F., McKnight S.L. 1988; The leucine zipper; a hypothetical structure common to a new class of DNA binding proteins.. Science 240:1759–1764
    [Google Scholar]
  18. Matsutani S., Ohtsubo H., Maeda Y., Ohtsubo E. 1987; Isolation and characterization of IS elements repeated in the bacterial chromosome.. J Mol Biol 196:445–455
    [Google Scholar]
  19. Miller J.H. 1972 Experiments in Molecular Genetics Cold Spring Harbor, NY: Cold Spring Harbor Laboratory;
    [Google Scholar]
  20. Pacciarini M.L., Savio J.L, Tagliabu S., Rossi G. 1992; Repetitive sequences cloned from Leptospira interrogans serovar hardjo genotype hardjoprajitno and their application to serovar identification.. J Clin Microbiol 30:1243–1249
    [Google Scholar]
  21. Polard P., Prere M.F., Chandler M., Fayet O. 1991; Programmed translational frameshifting and initiation at an AUU codon in gene expression of bacterial insertion sequence IS911.. J Mol Biol 222:465–477
    [Google Scholar]
  22. Polard P., Prere M.F., Fayet O., Chandler M. 1992; Transposase-induced excision and circularization of the bacterial insertion sequence 1S911.. EMBO J 11:5079–5092
    [Google Scholar]
  23. Polzin K.M., McKay L.L. 1991; Identification, DNA sequence, and distribution of IS981, a new, high-copy-number insertion sequence in Lactococci. . Appl Environ Microbiol 57:734–743
    [Google Scholar]
  24. Prere M.F., Chandler M., Fayet O. 1990; Transposition in Shigella dysenteriae: isolation and analysis of IS911, a new member of the IS3 group of insertion sequences.. J Bacteriol 172:4090–4099
    [Google Scholar]
  25. Ralph D., McClelland M. 1993; Intervening sequence with conserved open reading frame in eubacterial 23S rRNA genes.. Proc Natl Acad Sci USA 906864–6868
    [Google Scholar]
  26. Ramadass P., Jarvis B.D.W., Corner R.J., Penny D., Marshall R.B. 1992; Genetic characterization of pathogenic Leptospira species by DNA hybridization.. Int J Syst Bacteriol 42:215–219
    [Google Scholar]
  27. Saint Girons I., Norris S.J., Göbel U., Meyer J., Walker E.M., Zuerner R. 1992; Genome structure of spirochetes.. Res Microbiol 143:615–621
    [Google Scholar]
  28. Savio M.L., Rossi C., Fusi P., Tabliabue S., Pacciarini M.L. 1994; Detection and identification of Leptospira interrogans serovars by PCR coupled with restriction endonuclease analysis ofamplified DNA.. J Clin Microbiol 32:935–941
    [Google Scholar]
  29. Sanger F., Nicklen S., Coulson A.R. 1977; DNA sequencing with chain-terminating inhibitors.. Proc Natl Acad Sci USA 745463–5467
    [Google Scholar]
  30. Schwartz E., Kröger M., Rak B. 1988; IS 150: distribution, nucleotide sequence and phylogenetic relationships of a new E. coli insertion element.. Nucleic Acids Res 16:6789–6802
    [Google Scholar]
  31. Sekine Y., Eisaki N., Ohtsubo E. 1994; Translational control in production of transposase and in transposition of insertion sequence IS3.. J Mol Biol 235:1406–1420
    [Google Scholar]
  32. Shimizu-Kadota M., Kiwaki M., Hirokawa H., Tsuchida N. 1985; ISL1: a new transposable element in Lactobacillus casei. . Mol & Gen Genet 200:193–198
    [Google Scholar]
  33. Skaugen M., Nes I.F. 1994; Transposition in Lactobacillus sake and its abolition of lactocin S production by insertion of IS1163, a new member of the I S3 family.. Appl Environ Microbiol 60:2818–2825
    [Google Scholar]
  34. Timmerman K.P., Tu C.P.D. 1985; Complete sequence of IS3.. Nucleic Acids Res 13:2127–2139
    [Google Scholar]
  35. Umeda M., Ohtsubo E. 1989; Mapping of insertion elements IS1, IS2, and IS3 on the Escherichia coli K-12 chromosome. Role of the insertion elements in formation of Hfrs and F´ factors and in rearrangement of bacterial chromosomes.. J Mol Biol 208:601–614
    [Google Scholar]
  36. Van Eys G.J.J.M., Zall J., Schoone G.J., Terpstra W.J. 1988; DNA hybridization with hardjobovis specific recombinant probes as a method for type discrimination of Leptospira interrogans serovar hardjo. . J Gen Microbiol134–574
    [Google Scholar]
  37. Vögele K., Schwartz E., Welz C., Schiltz E., Rak B. 1991; High-level ribosomal frameshifting directs the synthesis of IS150 gene products.. Nucleic Acids Res 19:4377–4385
    [Google Scholar]
  38. Volkert M.R., Loewen P.C., Switala J., Crowley D., Conley M. 1994; The Δ(argF-lacZ)205(U169) deletion greatly enhances resistance to hydrogen peroxide in stationary-phase Escherichia coli. . J Bacteriol 176:1297–1302
    [Google Scholar]
  39. Woodward M.J., Sullivan G.J. 1991; Nucleotide sequence of a repetitive element isolated from Leptospira interrogans serovar hardjo type hardjo-bovis. . J Gen Microbiol 137:1101–1109
    [Google Scholar]
  40. Yasuda B.H., Steigerwalt A.G., Sulzer K.R., Kaufmann A.F., Rogers F., Brenner D.J. 1987; Deoxyribonucleic acid relatedness between serogroups and serovars in the family Leptospiraceae with proposals for seven new Leptospira species.. Int J Sjist Bacteriol 37:407–415
    [Google Scholar]
  41. Zuerner R.L. 1991; Physical map of chromosomal and plasmid DNA comprising the genome of Leptospira interrogans. . Nucleic Acids Res 19:4857–4860
    [Google Scholar]
  42. Zuerner R.L. 1993; Genomic structure, organization, and rearrangement in Leptospira. . J Cell Biochem S17E:309
    [Google Scholar]
  43. Zuerner R.L. 1994; Nucleotide sequence analysis of IS/333 from Leptospira borgpetersenii-. identification and expression of two IS- encoded proteins.. Plasmid 31:111
    [Google Scholar]
  44. Zuerner R.L., Bolin C.A. 1988; Repetitive sequence element cloned from Leptospira interrogans serovar hardjo type hardjo-bovis provides a sensitive diagnostic probe for bovine leptospirosis.. J Clin Microbiol 26:2495–2500
    [Google Scholar]
  45. Zuerner R.L., Bolin C.A. 1990; Nucleic acid probe characterizes Leptospira interrogans serovars by restriction fragment length polymorphisms.. Vet Microbiol 24:355–366
    [Google Scholar]
  46. Zuerner R.L, Ellis W.A., Bolin C.A., Montgomery J.M. 1993a; Restriction fragment length polymorphisms distinguish Leptospira borgpetersenii serovar hardjo type hardjo-bovis isolates from different geographical locations.. J Clin Microbiol 31:578–583
    [Google Scholar]
  47. Zuerner R.L, SaintGirons I. 1993b; Comparison of genetic maps for two Leptospira interrogans serovars provides evidence for two chromosomes and intraspecies heterogeneity.. J Bacteriol 175:5445–5451
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/13500872-141-9-2165
Loading
/content/journal/micro/10.1099/13500872-141-9-2165
Loading

Data & Media loading...

Most cited Most Cited RSS feed