Copies of an insertion-sequence (IS)-like element were isolated from two closely related serovars of Leptospira interrogans sensu stricto. Nucleotide sequence analysis of the 1236 bp element showed a characteristic IS structure with terminal imperfect inverted repeats (IRs) flanking a 1159 bp central region. This element was designated IS 1500. Four open reading frames (orfA-orfD) were found in the central ‘unique’ region of IS 1500. Similarities were detected between ORFA and ORFB and the putative transposases from members of the IS3 family of transposable elements. IS 1500 or IS 1500-like sequences were also detected in all other pathogenic leptospiral serovars, but not in the saprophytic species L. biflexa. Differences in IS1500 copy numbers in members of the same species suggest that this element can transpose. Physical mapping of IS 1500 insertions in L. interrogans serovars icterohaemorrhagiae and pomona showed insertions were only on the large chromosomal replicon. The location of some IS1500 insertions coincides with regions of the genome that have undergone large rearrangements.
BarilC.,
HerrmannJ.L.,
RichaudC.,
MargaritaD.,
Saint GironsI.1992; Scattering of the rRNA genes on the physical map of the circular chromosome of Leptospira interrogans serovar icterohaemorrhagiae.. J Bacteriol 23:7566–7571
BonamyC,
LabarreJ.,
ReyesO.,
LeblonG.1994; Identification of IS 1206, a Corynebacteriumglutamicum ISJ-related insertion sequence and phylogenetic analysis.. Mol Microbiol 14:571–581
ChiouC.-S.,
JonesA.L.1993; Nucleotide sequence analysis of a transposon (TnS393) carrying streptomycin resistance genes in Erwinia amylovora and other gram-negative bacteria.. J Bacteriol 175:732–740
DoakT.G.,
DoerderF.P.,
JahnC.L>
,
HerrickG.1994; A proposed superfamily of transposase genes: tranposon-like elements in ciliated protozoa and a common ‘D35E’ motif.. Proc Natl Acad Sci USA91942–946
EllinghausenH.C.,
McCulloughW.G.1965; Nutrition of Leptospira pomona and growth of 13 other serotypes: fractionation of oleic albumin complex and medium bovine albumin and polysorbate 80.. Am J Vet Res 26:45–51
FayetO.,
RamondP.,
PolardP.,
PrereM.F.,
ChandlerM.1990; Functional similarities between retroviruses and the ISJ family of bacterial insertion sequences.. Mol Microbiol 4:1771–1774
GalasD.J.,
ChandlerM.1989; Bacterial insertion sequences.. In Mobile DNA pp. 109–162 Edited by
BergD. E.,
HoweM. M.
Washington, DC: American Society for Microbiology;
HerrmannJ.L,
BellengerE.,
PerolatP.,
BarantonG.,
Saint GironsI.1992; Pulsed-field gel electrophoresis of Notl digests of leptospiral DNA: a new rapid method of serovar identification.. J Clin Microbiol 30:1696–1702
KropinskiA.M.,
FarinhaM.A.,
JansonsI.1994; Nucleotide sequence of the Pseudomonas aeruginosa insertion sequence IS222: another member of the IS3 family.. Plasmid 31:222–228
LandschulzW.H.,
JohnsonP.F.,
McKnightS.L.1988; The leucine zipper; a hypothetical structure common to a new class of DNA binding proteins.. Science 240:1759–1764
MatsutaniS.,
OhtsuboH.,
MaedaY.,
OhtsuboE.1987; Isolation and characterization of IS elements repeated in the bacterial chromosome.. J Mol Biol 196:445–455
PolardP.,
PrereM.F.,
ChandlerM.,
FayetO.1991; Programmed translational frameshifting and initiation at an AUU codon in gene expression of bacterial insertion sequence IS911.. J Mol Biol 222:465–477
PolzinK.M.,
McKayL.L.1991; Identification, DNA sequence, and distribution of IS981, a new, high-copy-number insertion sequence in Lactococci.
. Appl Environ Microbiol 57:734–743
PrereM.F.,
ChandlerM.,
FayetO.1990; Transposition in Shigella dysenteriae: isolation and analysis of IS911, a new member of the IS3 group of insertion sequences.. J Bacteriol 172:4090–4099
RamadassP.,
JarvisB.D.W.,
CornerR.J.,
PennyD.,
MarshallR.B.1992; Genetic characterization of pathogenic Leptospira species by DNA hybridization.. Int J Syst Bacteriol 42:215–219
SchwartzE.,
KrögerM.,
RakB.1988; IS 150: distribution, nucleotide sequence and phylogenetic relationships of a new E. coli insertion element.. Nucleic Acids Res 16:6789–6802
SekineY.,
EisakiN.,
OhtsuboE.1994; Translational control in production of transposase and in transposition of insertion sequence IS3.. J Mol Biol 235:1406–1420
SkaugenM.,
NesI.F.1994; Transposition in Lactobacillus sake and its abolition of lactocin S production by insertion of IS1163, a new member of the I S3 family.. Appl Environ Microbiol 60:2818–2825
UmedaM.,
OhtsuboE.1989; Mapping of insertion elements IS1, IS2, and IS3 on the Escherichia coli K-12 chromosome. Role of the insertion elements in formation of Hfrs and F´ factors and in rearrangement of bacterial chromosomes.. J Mol Biol 208:601–614
Van EysG.J.J.M.,
ZallJ.,
SchooneG.J.,
TerpstraW.J.1988; DNA hybridization with hardjobovis specific recombinant probes as a method for type discrimination of Leptospira interrogans serovar hardjo.
. J Gen Microbiol134–574
WoodwardM.J.,
SullivanG.J.1991; Nucleotide sequence of a repetitive element isolated from Leptospira interrogans serovar hardjo type hardjo-bovis.
. J Gen Microbiol 137:1101–1109
YasudaB.H.,
SteigerwaltA.G.,
SulzerK.R.,
KaufmannA.F.,
RogersF.,
BrennerD.J.1987; Deoxyribonucleic acid relatedness between serogroups and serovars in the family Leptospiraceae with proposals for seven new Leptospira species.. Int J Sjist Bacteriol 37:407–415
ZuernerR.L.
1994; Nucleotide sequence analysis of IS/333 from Leptospira borgpetersenii-. identification and expression of two IS- encoded proteins.. Plasmid 31:111
ZuernerR.L.,
BolinC.A.1988; Repetitive sequence element cloned from Leptospira interrogans serovar hardjo type hardjo-bovis provides a sensitive diagnostic probe for bovine leptospirosis.. J Clin Microbiol 26:2495–2500
ZuernerR.L,
SaintGironsI.1993b; Comparison of genetic maps for two Leptospira interrogans serovars provides evidence for two chromosomes and intraspecies heterogeneity.. J Bacteriol 175:5445–5451