RT Journal Article SR Electronic(1) A1 Mullins, Paul H. A1 Gürtler, Hanne A1 Wellington, Elizabeth M. H.YR 1995 T1 Selective recovery of Streptosporangium fragile from soil by indirect immunomagnetic capture JF Microbiology, VO 141 IS 9 SP 2149 OP 2156 DO https://doi.org/10.1099/13500872-141-9-2149 PB Microbiology Society, SN 1465-2080, AB A polyclonal antibody raised to Streptosporangium fragile spores reacted strongly and specifically with the immunizing strain and to a number of related species of Streptosporangium, as determined by dot immunoblotting. An indirect immunomagnetic capture method was developed for the recovery of the target organism from sterile and non-sterile soil, using sheep anti-rabbit M-280 Dynabeads. The effects of different soil blocking agents, antibody labelling concentrations and spore/Dynabead capture times on the recovery of S. fragile spores were investigated. Pre-blocking of antibody binding sites within the soil, with either 2% partially hydrolysed gelatin or 10% skimmed milk, was essential prior to immunomagnetic capture. Increasing the capture time from 15 to 60 min did not affect spore recovery; however, a 10-fold decline in the magnetic bead concentration did result in a significantly lower recovery of spores from soil. S. fragile was selectively enriched (1:190-fold) when present as a mixed population with Arthrobacter oxydans in sterile soil. The indirect immunomagnetic capture method was used to selectively recover S. fragile spores seeded into non-sterile soil, although some background binding of non-target bacteria was noted. The target was successfully recovered from a sterile soil microcosm after 14 d incubation and the capture rate was increased by the inclusion of an initial soil dispersion and biomass concentration procedure, using the ion-exchange resin Chelex 100., UL https://www.microbiologyresearch.org/content/journal/micro/10.1099/13500872-141-9-2149