Induction of heat shock response in Free

Abstract

General properties of the heat shock response in were examined. Enhanced or synthesis of 24 proteins was observed upon heat shock from 30 °C to 42°C in cells labelled with [S]methionine. A similar response could also be induced by a rise in temperature from 30 °C to 37 °C. Of these heat shock proteins, two were determined to be homologues of GroEL and DnaK, based upon their immunological cross-reactivity with antibodies raised against the proteins. Three proteins, of molecular sizes 38, 44 and 48 kDa, which were undetectable in the 30 °C grown culture, appeared after the heat shock. As in other prokaryotic systems thermal induction of many of the proteins was transient, but both DnaK and GroEL remained induced for at least 28 min after heat shock. DNA hybridization studies revealed that genes analogous not only to and but also to of exist in . Heat shock induced thermotolerance in but made the cells more sensitive to UV radiation. Unlike in , however, heat shock had no effect on the progeny virus yield in .

Keyword(s): DnaK , GroEL , heat shock and Vibrio cholerae
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1995-09-01
2024-03-29
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