1887

Abstract

The gene encoding a 51 kDa polypeptide of 381 was isolated by immunoblotting using an antiserum raised against alkaline phosphatase. DNA sequence analysis of a 2.5 kb DNA fragment containing a gene encoding the 51 kDa protein revealed one complete and two incomplete ORFs. Database searches using the FASTA program revealed significant homology between the 51 kDa protein and the MurC protein of , which functions in peptidoglycan synthesis. The cloned 51 kDa protein encoded a functional product that complemented an mutant. Moreover, the ORF just upstream of coded for a protein that was 31% homologous with the MurG protein. The ORF just downstream of coded for a protein that was 17% homologous with the penicillin-binding protein 2B (PBP2B), which functions in peptidoglycan synthesis and is responsible for antibiotic resistance. These results suggest that contains a homologue of the peptidoglycan synthesis gene and indicate the possibility of a cluster of genes responsible for cell division and cell growth, as in the region.

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1995-09-01
2021-08-02
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References

  1. Dickinson D.P., Kubiniec M.A., Yoshimura F., Genco R.J. 1988; Molecular cloning and sequencing of the gene encoding the fimbrial subunit protein of Bacteroides gingivalis. . J Bacteriol 170:1658–1665
    [Google Scholar]
  2. Hawkes R., Niday E., Gordon J. 1982; A dot-immunobinding assay for monoclonal and other antibodies.. Anal Biochem 119:142–147
    [Google Scholar]
  3. Hunter N. 1984; The interaction of bacterial peptidoglycan with macrophages in chronic inflammation.. J Dent Res 63:427–430
    [Google Scholar]
  4. Ikeda M., Wachi M., Jung H.K., Ishino F., Matsuhashi M. 1990; Nucleotide sequence involving murG and murC in the mra gene cluster region of Escherichia coli. . Nucleic Acids Res 18:4014
    [Google Scholar]
  5. Ishino F., Jung H.K., Ikeda M., Doi M., Wachi M., Matsuhashi M. 1989; New mutations fts-36, lts-33, and ftsW clustered in the mra region of the Escherichia coli chromosome induce thermosensitive cell growth and division.. J Bacteriol 171:5523–5530
    [Google Scholar]
  6. Laemmli U.K. 1970; Cleavage of structural proteins during the assembly of the head of bacteriophage T4.. Nature 227:680–685
    [Google Scholar]
  7. Maniatis T., Fritsch E.F., Sambrook J. 1982 Molecular Cloning: a Laboratory Manual. Cold Spring Harbor, NY:: Cold Spring Harbor Laboratory.;
    [Google Scholar]
  8. Marck C. 1988; ‘DNA strider’: a ‘C’ program for the fast analysis of DNA and protein sequences on the Apple Macintosh family of computers.. Nucleic Acids Res 16:1829–1836
    [Google Scholar]
  9. Marmur J. 1961; A procedure for the isolation of deoxyribonucleic acid from microorganisms.. J Mol Biol 3:208–218
    [Google Scholar]
  10. Meyer T.F., Mlawer N., So M. 1982; Pilus expression in Neisseria gonorrhoeae involves chromosomal rearrangement.. Cell 30:45–52
    [Google Scholar]
  11. Minhas H., Greenman J. 1989; Production of cell-bound and vesicle-associated trypsin-like protease, alkaline phosphatase and N-acetyl-β glucosaminidase by Bacteroides gingivalis strain W50.. J Gen Microbiol 135:557–564
    [Google Scholar]
  12. Miyakawa T., Matsuzawa H., Matsuhashi M., Sugino Y. 1972; Cell wall peptidoglycan mutants of Escherichia coli K-12: existence of two clusters of genes, mra and mrb, for cell wall peptidoglycan biosynthesis.. J Bacteriol 112:950–958
    [Google Scholar]
  13. Nanninga N. 1991; Cell division and peptidoglycan assembly in Escherichia coli. . Mol Microbiol 5:791–795
    [Google Scholar]
  14. Pearson W.G., Lipman D.J. 1988; Improved tools for biological sequence comparison.. Proc Natl Acad Sci USA 852444–2448
    [Google Scholar]
  15. Rogers H.J., Perkins H.R., Ward J.B. 1980; Biosynthesis of peptidoglycan.. In Microbial Cell Walls and Membranes pp. 239–297 Rogers H. J., Perkins H. R., Ward J.B. Edited by London:: Chapman & Hall.;
    [Google Scholar]
  16. Sanger F., Nickien S., Coulson A.R. 1977; DNA sequencing with chain-terminating inhibitors.. Proc Natl Acad Sci USA 745463–5467
    [Google Scholar]
  17. Slots J. 1977; The predominant cultivable microflora of advanced periodontitis.. Scand J Dent Res 85:114–121
    [Google Scholar]
  18. Slots J., Genco R.J. 1984; Black-pigmented Bacteroides species, Capnocytophaga species, and Actinobacillus actinomycetemcomitans in human periodontal disease: virulence factors in colonization, survival, and tissue destruction.. J Dent Res 63:412–421
    [Google Scholar]
  19. Stormo G.D. 1986; Translation initiation.. In Maximizing Gene Expression pp. 195–224 Reznikoff W., Gold L. Edited by Boston, MA:: Butterworth.;
    [Google Scholar]
  20. Tanner A.C.R., Haffer C., Bratthall G.T., Visconti R.A., Socransky S.S. 1979; A study of the bacteria associated with advancing periodontitis in man.. J Clin Periodontol 6:278–307
    [Google Scholar]
  21. Waxman D.J., Strominger J.L. 1983; Penicillin-binding proteins and the mechanism of action of β-lactam antibiotics.. Annu Rev Biochem 52:825–869
    [Google Scholar]
  22. Yamashita Y., Toyoshima K., Yamazaki M., Hanada N., Takehara T. 1990; Purification and characterization of alkaline phosphatase of Bacteroides gingivalis 381.. Infect Immun 58:2882–2887
    [Google Scholar]
  23. Yamashita Y., Takehara T., Kuramitsu H.K. 1993; Molecular characterization of a Streptococcus mutans mutant altered in environmental stress responses.. J Bacteriol 175:6220–6228
    [Google Scholar]
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