1887

Abstract

Forty intestinal spirochaete strains were investigated for nucleotide sequences related to the locus from by Southern hybridization of chromosomal DNA using the locus from strain P18A as a probe and by PCR using primers internal to the gene. The intensity of the hybridization signal at high stringency and positive PCR results suggested that 12 strains possessed a similar nucleotide sequence. PCR was negative for another 12 strains and the hybridization signal obtained from 11 of these was weak and one was negative. All strains hybridized under low stringency conditions. These results indicated that there is variation among the loci of strains. Among seven strains of and the proposed species ‘’ and ‘’, hybridization was weak and no PCR products were obtained, suggesting that these species have sequences related to, but divergent from, the sequences of strains of . Both gene probe hybridization and PCR analysis of nine strains of the proposed new genus ’, including isolates from pigs and humans, gave negative results.

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1995-09-01
2021-07-26
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References

  1. Andrews J.J., Hoffman L.J. 1982; A porcine colitis caused by a weakly beta-haemolytic treponeme (Treponema innocens?). . In Proceedings of the 25th Annual Meeting of the American Association of Veterinary Laboratory Diagnostics p. 395
    [Google Scholar]
  2. Barrett S.P. 1990; Intestinal spirochaetes in a Gulf Arab population.. Epidemiol Infect 104:261–266
    [Google Scholar]
  3. Binek M., Szynkiewicz Z.M. 1984; Physiological properties and classification of strains of Treponema sp. isolated from pigs in Poland.. Comp Immunol Microbiol Infect Dis 7:141–148
    [Google Scholar]
  4. Combs B.G., Hampson D.J., Harders S.J. 1992; Typing of Australian isolates of Treponema hyodysenteriae by serology and by restriction endonuclease analysis.. Vet Microbiol 31:273–285
    [Google Scholar]
  5. Dower W.J., Miller J.F., Ragsdale C.W. 1988; High efficiency transformation of E. coli by high voltage electroporation.. Nucleic Acids Res 16:6127–6145
    [Google Scholar]
  6. Harris D.L., Glock R.D., Christiansen C.R., Kinyon J.M. 1972; Swine dysentery I. Inoculation of pigs with Treponema hyodysenteriae (new species) and reproduction of the disease.. Vet Med Small Anim Clin 67:61–64
    [Google Scholar]
  7. Hudson M.J., Alexander T.J.L., Lysons R.J. 1976; Diagnosis of swine dysentery: spirochaetes which may be confused with Treponema hyodysenteriae. . Vet Rec 99:495–500
    [Google Scholar]
  8. Jacques M., Girard C., Higgins R., Goyette G. 1989; Extensive colonisation of the porcine colonic epithelium by a spirochaete similar to Treponema innocens. . J Clin Microbiol 27:1139–1141
    [Google Scholar]
  9. Kent K.A., Sellwood R., Lemcke R.M., Burrows M.R., Lysons R.J. 1989; Analysis of the axial filaments of Treponema hyodysenteriae by SDS-PAGE and immunoblotting.. J Gen Microbiol 135:1625–1632
    [Google Scholar]
  10. Kinyon J.M., Harris D.L. 1979; Treponema innocens, a new species of intestinal bacteria, and emended description of the type strain of Treponema hyodysenteriae. . Int J Syst Bacteriol 29:102–109
    [Google Scholar]
  11. Kinyon J.M., Harris D.L., Glock R.D. 1977; Entero- pathogenicity of various isolates of Treponema hyodysenteriae. . Infect Immun 15:638–646
    [Google Scholar]
  12. Lee J.1., Hampson D.J. 1992; Intestinal spirochaetes colonizing Aborigines from communities in the remote north of Western Australia.. Epidemiol. Infect 109:133–141
    [Google Scholar]
  13. Lee J.I., Hampson D.J. 1994; Genetic characterisation of intestinal spirochaetes and their association with disease.. J Med Microbiol 40:365–371
    [Google Scholar]
  14. Lee J.I., Hampson D.J., Combs B.G., Lymbery A.J. 1993a; Genetic relationships between isolates of Serpulina (Treponema) hyodysenteriae, and comparison of methods for their subspecific differentiation.. Vet Microbiol 34:35–46
    [Google Scholar]
  15. Lee J.I., Hampson D.J., Lymbery A.J., Harders S.J. 1993b; The porcine intestinal spirochaetes - identification of new genetic groups.. Vet Microbiol 34:273–285
    [Google Scholar]
  16. Lee J.I., McLaren A.J., Lymbery A.J., Hampson D.J. 1993c; Human intestinal spirochaetes are distinct from Serpulina hyodysenteriae. . J Clin Microbiol 31:16–21
    [Google Scholar]
  17. Lemcke R.M., Bew J. 1984; Antigenic differences among isolates of Treponema hyodysenteriae. . In Proceedings of the 8th International Pig Veterinary Society Congress Ghent, Belgium: p. 183
    [Google Scholar]
  18. Lemcke R.M., Burrows M.R. 1979; A disc growth-inhibition test for differentiating Treponema hyodysenteriae from other intestinal spirochaetes.. Vet Rec 104:548–551
    [Google Scholar]
  19. Lysons R.J., Hall G.A., Alexander T.J.L., Bew J., Bland A.P. 1978; Aetiological agents and pathogenesis of swine dysentery.. In Proceedings of the 5th International Pig Veterinary Society Congress Zagreb: p. M1
    [Google Scholar]
  20. Ruane P.J., Nakata M.M., Reinhardt J.F., George W.L. 1989; Spirochaete-like organisms in the human gastrointestinal tract.. Rev Infect Dis 11:184–196
    [Google Scholar]
  21. Sambrook J., Fritsch E.F., Maniatis T. 1989 Molecular Cloning: a Laboratory Manual, 2nd edn.. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory;
    [Google Scholar]
  22. Sanna A., Dettori G., Grillo R., Rossi A., Chiarenza D. 1982; Isolation and propagation of a strain of Treponema from the human digestive tract - preliminary report.. Ig Mod 77:287–297
    [Google Scholar]
  23. Sanna A., Dettori G., Agliano A.M., Branca G., Grillo R., Leone F., Rossi A., Parisi G. 1984; Studies of treponemes isolated from human gastrointestinal tract.. Ig Mod 81:959–973
    [Google Scholar]
  24. Scharf S.J., Higuchi R., Horn G.T., Mullis K.B., Erlich H.A. 1988; Primer-directed enzymatic amplification of DNA with a thermostable DNA polymerase.. Science 239:487–491
    [Google Scholar]
  25. Sellwood R., Kent K.A., Burrows M.R., Lysons R.J., Bland A.P. 1989; Antibodies to a common outer envelope antigen of Treponema hyodysenteriae with antibacterial activity.. J Gen Microbiol 135:2249–2257
    [Google Scholar]
  26. Silhavy T.J., Berman M.L., Enquist L.W. 1984 Experiments with Gene Fusions pp. 137–139 Cold Spring Harbor, NY: Cold Spring Harbor Laboratory;
    [Google Scholar]
  27. Taylor D.J., Alexander T.J.L. 1971; The production of dysentery in swine by feeding cultures containing a spirochaete.. Br Vet J 127:lviii–lxi
    [Google Scholar]
  28. Taylor D.J., Simmons J.R., Laird H.M. 1980; Production of diarrhoea and dysentery in pigs by feeding pure cultures of a spirochaete differing from Treponema hyodysenteriae. . Vet Rec 106:326–332
    [Google Scholar]
  29. Thomas W., Sellwood R. 1992; Monoclonal antibodies to a 16 kDa antigen of Serpulina (Treponema) hyodysenteriae. . J Med Microbiol 37:214–220
    [Google Scholar]
  30. Thomas W., Sellwood R. 1993; Molecular cloning, expression, and DNA sequence analysis of the gene that encodes the 16- kilodalton outer membrane lipoprotein of Serpulina hyodysenteriae. . Infect Immun 61:1136–1140
    [Google Scholar]
  31. Thomas W., Sellwood R., Lysons R.J. 1992; A 16 kilodalton lipoprotein of the outer membrane of Serpulina (Treponema) hyodysenteriae. . Infect Immun 60:3111–3116
    [Google Scholar]
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