1887

Abstract

has rough (R)-type lipopolysaccharide, containing 2-keto-3-deoxyoctonate but no heptoses. Its lipid A has a glucosamine-containing, phosphorylated backbone. It contains the rare 3-oxotetradecanoic (3-oxomyristic) acid as the only amide-bound fatty acid and ester-bound 3-hydroxydecanoic acid, this pattern being characteristic for the a 3 subgroup of Proteobacteria. Treatment of the major outer-membrane protein (porin, apparent molecular mass 88 kDa) of with EDTA (2 mM, 30°C, 20 min) resulted in the dissociation of the oligomers into monomers (apparent molecular mass 35 kDa). EDTA-sensitive dissociation has so far been observed only within the α-3 subgroup of Proteobacteria. The 12 N-terminal amino acids of the monomers exhibit sequence homology with the porins of and Renaming of Roseobacter denitrificans as is suggested.

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1995-08-01
2024-11-08
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References

  1. Brade H., Galanos C.H. 1983; A method to detect 2-keto-3-deoxyoctonate and related compounds on pherograms and chromatograms. Anal Biochem 132:158–159
    [Google Scholar]
  2. Evans W.R., Fleishman D.E., Calvert H.E., Pyaty P.V., Alter G.M., SubbaRao N.S. 1990; Bacteriochlorophyll and photosynthetic reaction centers in Rhiɀobium strain BTAil. Appl Environ Microbiol 56:3445–3449
    [Google Scholar]
  3. Fuerst J.A., Hawkins J.A., Holmes A., Sly L.I., Moore C.J., Stackebrandt E. 1993; Porphyrobacter neustonensis gen. nov., sp. nov., an aerobic bacteriochlorophyll-synthesizing budding bacterium from fresh water. Int J Syst Bacteriol 43:125–134
    [Google Scholar]
  4. Kawasaki H., Hoshino Y., Hirata A., Yamasato K. 1993; Is intracytoplasmic membrane structure a generic criterion? It does not coincide with phylogenetic interrelationships among phototrophic purple nonsulfur bacteria. Arch Microbiol 160:358–362
    [Google Scholar]
  5. Komuro T., Galanos C. 1988; Analysis of Salmonella LPS by sodium deoxycholate-polyacrylamide gel electrophoresis. J Chromatogr 450:381–387
    [Google Scholar]
  6. Krauss J.H., Seydel U., Weckesser J., Mayer H. 1989; Structural analysis of the nontoxic lipid A of Rhodobacter capsulatus37b4. Eur J Biochem 180:519–526
    [Google Scholar]
  7. Kreusch A., Neubüser A., Schiltz E., Weckesser J., Schulz G.E. 1994; Structure of the membrane channel porin from Rhodopseudomonas blastica at 2·0 A resolution. Protein Sci 3:58–63
    [Google Scholar]
  8. Liebetanz R., Hornberger U., Drews G. 1991; Organization of the gene coding for the reaction center L and M subunits and B870antenna polypeptides α and ▲ from the aerobic photosynthetic bacterium Erythrobacter sp. OCh 114. Mol Microbiol 5:1459–1468
    [Google Scholar]
  9. Lowry O.H., Roberts N.R., Kuner K.Y., Wu N.L., Farr A.L. 1954; The quantitative histochemistry of brain. Chemical methods I. J Biol Chem 207:1–17
    [Google Scholar]
  10. Mayer H., Weckesser J. 1984; ̒Unusual̓ lipid A̓s: structures, taxonomical relevance and potential value for endotoxin research. In Handbook of Endotoxin 1 Chemistry of Endotoxin pp. 221–247 Rietschel E.Th. Edited by Amsterdam:: Elsevier.;
    [Google Scholar]
  11. Mayer H., Masoud H., Urbanik-Sypniewska T., Weckesser J. 1989; Lipid A composition and phylogeny of Gram-negative bacteria. Bull Jpn Fed Cult Collect 5:19–25
    [Google Scholar]
  12. Okamura K., Miata T., Iwanaga S., Takamia K., Nishimura M. 1987; Complete amino acid sequence of cytochrome c551 from Erythrobacter species strain OCh 114. J Biochem 101:957–966
    [Google Scholar]
  13. Partridge S.M. 1948; Filter-paper partition chromatography of sugars. 1. General description and application to the qualitative analysis of sugars in apples, egg white and foetal blood of sheep. Biochem J 42:238–250
    [Google Scholar]
  14. Salimath P.V., Weckesser J., Strittmatter W., Mayer H. 1983; Structural studies on the non-toxic lipid A from Rhodopseudomonas sphaeroides ATCC 17023. Eur J Biochem 136:195–200
    [Google Scholar]
  15. Sato K. 1987; Bacteriochlorophyll formation by facultative methylotrophs, Protaminobacter ruber and Pseudomonas AMI. FEBS Lett 85:207–210
    [Google Scholar]
  16. Schiltz E., Kreusch A., Nestel U., Schulz G.E. 1991; Primary structure of porin from Rhodobacter capsulatus. Eur J Biochem 199:587–594
    [Google Scholar]
  17. Schnaitman C.A. 1971; Solubilization of the cytoplasmic membrane of Escherichia coli by Triton X-100. J Bacteriol 108:545–552
    [Google Scholar]
  18. Shiba T. 1991; Roseobacter litoralis gen. nov. sp. nov. and Roseobacter denitrificans sp. nov., aerobic pink-pigmented bacteria which contain bacteriochlorophyll a. Syst Appl Microbiol 14:140–145
    [Google Scholar]
  19. Shiba T., Shioi Y., Takamiya K., Sutton D.C., Wilkinson C.R. 1991; Distribution and physiology of aerobic bacteria containing bacteriochlorophyll a on the East and West coasts of Australia. Appl Environ Microbiol 57:295–300
    [Google Scholar]
  20. Shiba T., Simidu U. 1982; Erythrobacter longus gen. nov., sp. nov., an aerobic bacterium which contains bacteriochlorophyll a. Int J Syst Bacteriol 32:211–217
    [Google Scholar]
  21. Shioi Y. 1986; Growth characteristics and substrate specifity of aerobic photosynthetic bacterium Erythrobacter sp. (OCh 114). Plant Cell Physiol 27:567–572
    [Google Scholar]
  22. Snyder F., Stephens N. 1959; A simplified spectrophotometric determination of ester groups in lipids. Biochim Biophys Acta 34:244–245
    [Google Scholar]
  23. Stackebrandt E., Murray R.G.E., Trüper H.G. 1988; Proteobacteria classis nov., a name for the phylogenetic taxon that includes the “purple bacteria and their relatives*#x201D;. Int J Syst Bacteriol 38:312–325
    [Google Scholar]
  24. Stead A., Main J.S., Ward M.E., Watt P.J. 1975; Studies on lipopolysaccharides isolated from strains of Neisseria gonorrhoeae. J Gen Microbiol 88:123–131
    [Google Scholar]
  25. Tegtmeyer B., Weckesser J., Mayer H., Imhoff J.F. 1985; Chemical composition of the lipopolysaccharides of Rhodobacter sulfidophilus, Rhodopseudomonas acidophila and Rhodopseudomonas blastica. Arch Microbiol 143:32–36
    [Google Scholar]
  26. Weckesser J., Mayer H. 1988; Different lipid A types in lipopolysaccharides of phototrophic and related non-phototrophic bacteria. FEMS Microbiol Rev 54:143–154
    [Google Scholar]
  27. Weckesser J., Mayer H., Schulz G.E. 1995; Anoxygenic phototrophic bacteria: model organisms for studies on cell wall macromolecules. In Anoxygenic Photosynthetic Bacteria Blankenship R.E., Madigan M.T., Bauer C.E. Edited by Dordrecht:: Kluwer (in press).;
    [Google Scholar]
  28. Weiss M.S., Abele U., Weckesser J., Welte W., Schiltz E., Schulz G.E. 1991; Molecular architecture and electrostatic properties of a bacterial porin. Science 254:1627–1630
    [Google Scholar]
  29. Westphal O., Jann K. 1965; Bacterial lipopolysaccharides, extraction with phenol/water and further application of the procedure. Methods Carbohydr Chem 5:83–91
    [Google Scholar]
  30. Woese C.R. 1987; Bacterial evolution. Microbiol Rev 51:221–227
    [Google Scholar]
  31. Woese C.R., Stackebrandt E., Weisburg W.G., Paster B.J., Madigan M.T., Fowler V.J., Hahn C.M., Blanz P., Gupta R., Nealson K.H., Fox G.E. 1984; The phylogeny of the purple bacteria: the alpha subdivision. Syst Appl Microbiol 5:315–326
    [Google Scholar]
  32. Woitzik D., Weckesser J., Benz R., Stevanovic S., Jung G., Rosenbusch J.P. 1990; Porin of Rhodobacter capsulatus: biochemical and functional characterization. Z Naturforsch 45c:576–582
    [Google Scholar]
  33. Yao K., Ubuka T., Masuoka N., Kinuta N., Ikeda T. 1989; Direct determination of bound sialic acids in sialoglycoproteins by acidic ninhydrin reaction. Anal Biochem 179:332–335
    [Google Scholar]
  34. Yurkov V., Gorlenko V.M. 1992; A new genus of freshwater aerobic bacteriochlorophyll a-containing bacteria, Roseococcus gen. nov. Microbiology 60:628–632
    [Google Scholar]
  35. Yurkov V., Gorlenko V.M., Kompantseva E.I. 1992; A new type of fresh water orange-colored bacterium Erythromicrobium gen. nov. Microbiology 61:169–172
    [Google Scholar]
  36. Yurkov V., van Gemerden H. 1993; Abundance and salt tolerance of obligately aerobic, phototrophic bacteria in a microbial mat. Netherlands J Sea Res 31:57–62
    [Google Scholar]
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