1887

Abstract

In this paper we describe the nucleotide sequence of a 3·4 kbp region of the genome. This region contains an open reading frame of 1290 bp with a coding capacity for a protein of 46179 Da, designated the 38L protein. Using antibodies against part of the 38L protein, we were able to demonstrate that the 38L protein is present in the membrane protein of The 38L protein showed significant matches with a number of integral membrane proteins involved in the transport of small molecules through the cellular membrane. Among these are a human and a murine protein involved in melanin biosynthesis. The 38L protein might play a role in the hypopigmentation observed in leprosy patients.

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1995-08-01
2021-10-16
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References

  1. Chen C.-M., Misra T.K., Silver S., Rosen B.P. 1986; Nucleotide sequence of the structural genes for an anion pump. J Biol Chem 261:15030–15038
    [Google Scholar]
  2. Clark-Curtiss J.E., Jacobs W.R., Docherty M.A., Ritchie L.R., Curtiss R. III 1985; Molecular analysis of DNA and construction of genomic libraries of Mycobacterium leprae. . J Bacteriol 161:1093–1102
    [Google Scholar]
  3. Devereux J., Haeberli P., Smithies O. 1984; A comprehensive set of sequence analysis programs for the VAX. Nucleic Acids Res 12:387–395
    [Google Scholar]
  4. Eiglmeier K., Honoré N., Woods S.A., Caudron B., Cole S.T. 1993; Use of an ordered cosmid library to deduce the genomic organization of Mycobacterium leprae. . Mol Microbiol 1:197–206
    [Google Scholar]
  5. Gardner J.M., Nakatsu Y., Gondo Y., Lee S., Lyon M.F., King R.A., Brilliant M.H. 1992; The mouse pink-eyed dilution gene : association with human Prader-Willi and Angelman syndromes. Science 257:1121–1124
    [Google Scholar]
  6. Gastambide-Odier M., Sarda P., Lederer E. 1967; Biosynth°se des aglycones des mycosides A et B. Bull Soc Chim Biol 49:849–864
    [Google Scholar]
  7. Gaylord H., Brennan P.J. 1987; Leprosy and the leprosy bacillus: recent developments in the characterization of antigens and immunology of the disease. Annu Rev Microbiol 41:645–675
    [Google Scholar]
  8. Hunter S.W., Rivoire B., Mehra V., Bloom B.R., Brennan P.J. 1990; The major native proteins of the leprosy bacillus. J Biol Chem 265:14065–14068
    [Google Scholar]
  9. Ji G., Silver S. 1992; Regulation and expression of the arsenic resistance operon from Staphylococcus aureus plasmid pI258. J Bacteriol 174:3684–3694
    [Google Scholar]
  10. Klein P., Kanehisa M., DeLisi C. 1985; The detection and classification of membrane spanning proteins. Biochim Biophys Acta 815:468–476
    [Google Scholar]
  11. Kyte J., Doolittle R.F. 1982; A simple method for displaying the hydropathic character of a protein. J Mol Biol 157:105–132
    [Google Scholar]
  12. Laemmli U.K. 1970; Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227:680–685
    [Google Scholar]
  13. Matsuyama S.-I., Akimaru J., Mirushima S. 1990; SecE- dependent overproduction of SecY in Escherichia coli. . FEBS Lett 269:96–100
    [Google Scholar]
  14. Mehra V., Bloom B.R., Torigian V.K., Mandich D., Reichel M., Young S.M.M., Salgame P., Convit J., Hunter S.W., McNeil M., Brennan P.J., Rea T.H., Modlin R.L. 1989; Characterization of Mycobacterium leprae cell wall-associated proteins with the use of T lymphocyte clones. J Immunol 142:2873–2878
    [Google Scholar]
  15. Melancon-Kaplan J., Hunter S.W., McNeil M., Stewart C, Rea T.H., Convit J., Salgame P., Mehra V., Bloom B.R., Brennan P.J. 1988; Immunological significance of Mycobacterium leprae cell walls. Proc Natl Acad Sci USA 85:1917–1921
    [Google Scholar]
  16. Nordeen S.K., Bravo L.L., Sundaresan T.K. 1992; Estimated number of leprosy cases in the world. Bull WHO 70:7–10
    [Google Scholar]
  17. Parker M. 1988; Hypopigmentation in leprosy: its mechanism and significance. In Essays on Leprosy pp. 101–123 Ryan T.J., McDougall A.C. Edited by Oxford: Slade Hospital Department of Dermatology;
    [Google Scholar]
  18. Pinner E., Padan E., Schuldiner S. 1992; Cloning, sequencing and expression of the nhaB gene, encoding a Na+/H+ antiporter in Escherichia coli. . J Biol Chem 267:11064–11068
    [Google Scholar]
  19. Ridley D.S., Joplin W.H. 1966; Classification of leprosy according to immunity. A five-group system. Int J Lepr 34:255–273
    [Google Scholar]
  20. Rinchik E.M., Bultman S.J., Horsthemke B., Lee S.-T., Strumk K.M., Spritz R.A., Avidano K.M., Jong M.T.C., Nicholls R.D. 1993; A gene for the mouse pink-eyed dilution locus and for human type II oculocutaneous albinism. Nature 361:72–76
    [Google Scholar]
  21. Roche P.W., Neupane K.D., Britton W.J. 1992; Cellular immune response to the cell walls of Mycobacterium leprae in leprosy patients and healthy subjects exposed to leprosy. Clin Exp Immunol 89:110–114
    [Google Scholar]
  22. Sambrook J., Fritsch E.F., Maniatis T. 1989 Molecular Cloning-, a Laboratory Manual, 2. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory;
    [Google Scholar]
  23. Shereef P.H. 1992; Hypopigmented macules in leprosy-a histopathological and histochemical study of melanocytes. Indian J Lepr 64:189–191
    [Google Scholar]
  24. Silver S., Ji G., Bröer S., Dey S., Dou D., Rosen B.P. 1993; Orphan enzyme or patriarch of a new tribe: the arsenic resistance ATPase of bacterial plasmids. Mol Microbiol 8:637–642
    [Google Scholar]
  25. Swank R.T., Munkres K.D. 1971; Molecular weight analysis of oligopeptides by electrophoresis in polyacrylamide gel with sodium dodecyl sulphate. Anal Biochem 39:462–477
    [Google Scholar]
  26. Westerhof W. 1977; A possible dysfunction of melanosome transfer in leprosy: an electron-microscopic study. Acta Dermato- venerol(Stockholm) 57:297–304
    [Google Scholar]
  27. Wheeler P.R. 1989; Biosynthetic pathways in Mycobacterium leprae. . Acta Leproll 7: S1 21–24
    [Google Scholar]
  28. Yazyu H., Shiota-Niiya S., Shimamoto T., Kanazawa H., Futai M., Tsuchiya T. 1984; Nucleotide sequence of the me IB gene and characteristics of the deduced amino acid sequence of the melibiose carrier in Escherichia coli. . J Biol Chem 259:4320–4326
    [Google Scholar]
  29. Young D.B., Kaufmann S.H.E., Hermans P.W., Thole J.E.R. 1992; Mycobacterial protein antigens: a compilation. Mol Microbiolb 6:133–145
    [Google Scholar]
  30. Young R.A., Mehra V., Sweetser D., Buchanan T., Clark-Curtiss J., Davis R.W., Bloom B.R. 1985; Genes for the major protein antigens of the leprosy parasite Mycobacterium leprae. . Nature 316:450–452
    [Google Scholar]
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