The 168 circular chromosome yielded ten fragments on I-i endonuclease digestion. I-l recognizes a 26 bp sequence that is located within the gene encoding the 235 subunit of the rRNA in eugametos, and The precise locations of the I-l sites of the chromosome were determined on a l physical map by (i) double digestion analyses with I-l and l, (ii)comparison of mutant strains lacking a specific operon, (iii)using an I-l linking clone and (iv) analysis of nucleotide sequence data of some operons. In conclusion, all the I-l sites were located within the operons and the I-l sites were conserved in all the 168 derivatives tested. Thus, variations in size of the I-l fragments must be due to genome alterations. A 168 strain was investigated with I-l. We demonstrated that the aberrant structure was the outcome of the inversion of an ∼ 1700 kb DNA segment.


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