1887

Abstract

The gene, encoding a leucyl tRNA recognizing the UUA codon, is expressed at significantly lower levels in the mutant, J703, than in the parent A3(2). Expression of a TTA-containing reporter gene was reduced in the mutant, as was the mature, 87 nucleotide, form of the -encoded tRNA. This reduced level of the tRNA was also seen when the gene was introduced on a high-copy-number plasmid into the mutant, suggesting that maximal expression may require a -dependent promoter.

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1995-08-01
2021-09-25
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