A gene encoding a thermophilic alkaline serine proteinase from Thermus sp. strain Rt41A and its expression in Escherichia coli

References

1. Ævarsson A., Holst O., Kristjansson J.K. 1991; Growth behaviour and protease production by an Icelandic Thermus sp. isolate in batch and continuous culture.. Curr Microbiol 23:1–6
   [Google Scholar]
2. Bergquist P.L., Love D.R., Croft J.E., Streiff M.B, Daniel R.M., Morgan W.H. 1987; Genetics and potential biotechnological applications of thermophilic and extremely thermophilic micro-organisms.. Biotechnol Genet Eng Ren 5:199–244
   [Google Scholar]
3. Bjarnason J.B., Kristjánsson H., Olafsdóttir S., Gísladóttir G., Fox J.W., Kristjánsson J.K., Alfreósson G. 1992; Purification and characterisation of a thermophilic proteinase produced by Thermus strain IS-15.. In Thermophiles: Science and Technology IceTec: Reykjavík, Iceland;
   [Google Scholar]
4. Blumentals I.I., Robinson A.S., Kelly R.M. 1990; Characterisation of sodium dodecyl sulfate-resistant proteolytic activity in the hyperthermophilic archaebacterium Pyrococcus furiosus. . Appl Environ Microbiol 56:1992–1998
   [Google Scholar]
5. Borges K.M., Bergquist P.L. 1992; A rapid method for preparation of bacterial chromosomal DNA in agarose plugs using Thermus Rt41A proteinase.. Biotechniques 12:222–223
   [Google Scholar]
6. Coolbear T., Daniel R.M., Cowan D.A., Morgan H.W. 1988; Proteases from extreme thermophiles.. Enzyme Eng 9:279–281
   [Google Scholar]
7. Coolbear T., Daniel R.M., Morgan H.W. 1992; The enzymes from extreme thermophiles: bacterial sources, thermostabilities and industrial relevance.. InAdvances in Biochemical Engineering! Biotechnology 45 pp. 57–98 Edited by Fiechter A. Berlin: Springer-Verlag;
   [Google Scholar]
8. Cowan D.A., Daniel R.M., Morgan H.W. 1985; Thermophilic proteases: properties and potential applications.. Trends Biotechnol 3:68–72
   [Google Scholar]
9. Cowan D.A., Smolenski K.A., Daniel R.M., Morgan H.W. 1987a; An extremely thermostable extracellular proteinase from a strain of the archaebacterium Desulfurococcus growing at 88 °C.. Biochem J 247:121–133
   [Google Scholar]
10. Cowan D.A., Daniel R.M., Morgan H.W. 1987b; A comparison of extracellular serine proteases from four strains of Thermus aquaticus. . FEMS Microbiol Eett 43:155–159
    [Google Scholar]
11. Deane S.M., Robb F.T., Woods D.R. 1987; Production and activation of an SDS-resistant alkaline serine exoprotease of Vibrio alginolyticus. . J Gen Microbiol 133:391–398
    [Google Scholar]
12. Deane S.M., Robb F.T., Robb S.M., Woods D.R. 1989; Nucleotide sequence of the Vibrio alginolyticus calcium-dependent, detergent-resistant alkaline serine exoprotease A.. Gene 76:281–288
    [Google Scholar]
13. Devereux J., Haeberli P., Smithies 0. 1984; A comprehensive set of sequence analysis programs for the VAX.. Nucleic Acids Res 12:387–395
    [Google Scholar]
14. Frömmel C., Sander C. 1989; Thermitase, a thermostable subtilisin: comparison of predicted and experimental structures and the molecular cause of thermostability.. Proteins Struct Fund Genet 5:22–37
    [Google Scholar]
15. Fujishige A., Smith K.R., Silen J.L., Agard D.A. 1992; Correct folding of alpha-lytic protease is required for its extracellular secretion from Escherichia coli. . J Cell Biol 118:33–42
    [Google Scholar]
16. Fung M., Fung K.Y. 1991; PCR amplification of mRNA directly from a crude cell lysate prepared by thermophilic protease digestion.. Nucleic Acids Res 19:4300
    [Google Scholar]
17. Guan K.L., Dixon J.E. 1991; Eukaryotic proteins expressed in Escherichia coli-. an improved thrombin cleavage and purification procedure of fusion proteins with glutathione-S-transferase.. Anal Biochem 192:262–267
    [Google Scholar]
18. von Heijne G. 1983; Patterns of amino acids near signal-sequence cleavage sites.. Eur.J Biochem 133:17–21
    [Google Scholar]
19. Jacobs M., Eliasson M., Uhlen M., Flock J. 1985; Cloning, sequencing and expression of subtilisin Carlsberg from Bacillus licheniformis. . Nucleic Acids Res 13:8913–8926
    [Google Scholar]
20. Janssen P.H., Morgan H.W., Daniel R.M. 1991; Effects of medium composition on extracellular proteinase stability and yield in batch cultures of a Thermus sp.. Appl Microbiol Biotechnol 34:789–793
    [Google Scholar]
21. Jany K., Lederer G., Mayer B. 1986; Amino acid sequence of proteinase K from the mold Tritirachium album Limber. Proteinase K - a subtilisin-related enzyme with disulphide bonds.. FEBS Lett 199:139–144
    [Google Scholar]
22. Jones C.W., Morgan H.W., Daniel R.M. 1988; Aspects of protease production by Thermus strain Ok6 and other New Zealand isolates.. J Gen Microbiol 134:191–198
    [Google Scholar]
23. Kagawa Y., Nojima H., Nukiwa N., Ishizuka M., Nakajima T., Yasuhara T., Tanaka T., Oshima T. 1984; High guanine plus cytosine content in the third letter of codons of an extreme thermophile. DNA sequence of the isopropylmalate dehydrogenase of Thermus thermophilus. . J Biol Chem 259:2956–2960
    [Google Scholar]
24. Kobayashi T., Inouye M. 1992; Functional analysis of the intramolecular chaperone - mutational hot spots in the subtilisin pro-peptide and a 2nd-site suppressor mutation within the subtilisin molecule.. J Mol Biol 226:931–933
    [Google Scholar]
25. Kwon S., Terada I., Matsuzawa H., Ohta T. 1988; Nucleotide sequence of the gene for aqualysin I (a thermophilic alkaline serine protease) of Thermus aquaticus YT-1 and characteristics of the deduced primary structure of the enzyme.. Eur J Biochem 173:491–497
    [Google Scholar]
26. Lee Y.-G, Miyata Y., Terada I., Ohta T., Matsuzawa H. 1991; Involvement of NH-terminal pro-sequence in the production of active aqualysin I (a thermophilic serine protease) in Escherichia coli. . Agric Biol Chem 55:3027–3032
    [Google Scholar]
27. McHale R.H., Stapleton P.M., Bergquist P.L. 1991; A rapid method for the preparation of samples for PCR.. Biotechniques 10:21–22
    [Google Scholar]
28. Madver B., McHale R.H., Saul D.J., Bergquist P.L. 1994; Cloning and sequencing of a serine proteinase gene from a thermophilic Bacillus species and its expression in Escherichia coli. . Appl Environ Microbiol 60:3981–3988
    [Google Scholar]
29. Matsuzawa H., Hamaoki M., Ohta T. 1983; Production of thermophilic extracellular proteases (aqualysins I and II) by Thermus aquaticus YT-1, an extreme thermophile.. Agric Biol Chem 47:25–28
    [Google Scholar]
30. Meloun B., Baudys M., Kostka V., Hausdorf G., Frömmel C., Höhne W.E. 1985; Complete primary structure of thermitase from Thermoactinomyces vulgaris and its structural features related to the subtilisin-type proteinases.. FEBS Lett 183:195–200
    [Google Scholar]
31. Ohta Y., Inouye M. 1990; Pro-subtilisin E: purification and characterization of its autoprocessing to active subtilisin E in vitro. . Mol Microbiol 4:295–304
    [Google Scholar]
32. Pantoliano M.W., Ladner R.C, Bryan P.N., Rollence M.L., Wood J.F., Poulos T.L. 1987; Protein engineering of subtilisin BPN´, enhanced stabilization through the introduction of two cysteines to form a disulfide bond.. Biochemistry 26:2077–2082
    [Google Scholar]
33. Peek K., Daniel R.M., Monk G, Coolbear T. 1992; Purification and characterisation of a thermostable proteinase isolated from a Thermus sp. strain Rt41A.. Eur J Biochem 207:1035–1044
    [Google Scholar]
34. Samal B.B., Karan B., Boone T.G, Osslund T.D., Chen K.K., Stabinsky Y. 1990; Isolation and characterization of the gene encoding a novel, thermostable serine proteinase from the mould Tritirachium album Limber.. Mol Microbiol 4:1789–1792
    [Google Scholar]
35. Samal B.B., Karan B., Parker C., Stabinsky Y. 1991; Isolation and thermal studies of two novel serine proteinases from the fungus Tritirachium album Limber.. Enzyme Microb Technol 13:66–70
    [Google Scholar]
36. Saravani G., Cowan D.A., Daniel R.M., Morgan H.W. 1989; Caldolase, a chelator-insensitive extracellular serine proteinase from a Thermus sp.. Biochem J 262:409–416
    [Google Scholar]
37. Saul D.J., Rodrigo A.G., Reeves R.A., Williams L.G., Borges K.M., Morgan H.W., Bergquist P.L. 1993; Phylogeny of twenty Thermus isolates constructed from 16S rRNA gene sequence data.. Int J Syst Baderiol 43:754–760
    [Google Scholar]
38. Siezen R.J., de Vos W.M., Leunissen J.A.M., Dijkstra B.W. 1991; Homology modelling and protein engineering strategy of subtilases, the family of subtilisin-like serine proteinases.. Protein Eng 4:719–737
    [Google Scholar]
39. Skjenstad T., Hreggvidsson G.O., Gudjónsson T., Kristjánsson J.K. 1992; Isolation and some properties of a protease from Thermus ruber. . In Thermophiles-. Science and Technology IceTec: Reykjavik, Iceland;
    [Google Scholar]
40. Sorensen M.A., Kurland C.G., Pedersen S. 1989; Codon usage determines translation rate in Escherichia coli. . J Mol Biol 207:365–377
    [Google Scholar]
41. Souter N.H., Sharp R.J., Marks T.S. 1992; Characterisation of an alkaline serine protease from Thermus ruber. . In Thermophiles: Science and Technology IceTec: Reykjavik, Iceland;
    [Google Scholar]
42. Takagi H., Takahashi T., Momose H., lnouye M., Maeda Y., Matsuzawa H., Ohta T. 1990; Enhancement of the thermostability of subtilisin E by introduction of a disulfide bond engineered on the basis of structural comparison with a thermophilic serine protease.. J Biol Chem 265:6874–6878
    [Google Scholar]
43. Terada I., Kwon S., Miyata Y., Matsuzawa H., Ohta T. 1990; Unique precursor structure of an extracellular protease, aqualysin I, with NH₂- and COOH-terminal pro-sequences and its processing in Escherichia coli. . J Biol Chem 265:6576–6581
    [Google Scholar]
44. Touhara N., Taguchi H., Koyama Y., Ohta T., Matsuzawa H. 1991; Production and extracellular secretion of aqualysin-I (a thermophilic subtilisin-type protease) in a host-vector system for Thermus thermophilus. . Appl Environ Microbiol 57:3385–3387
    [Google Scholar]
45. Vasantha N., Thompson L.D., Rhodes C., Banner G, Nagle J., Filpula D. 1984; Genes for alkaline protease and neutral protease from bacillus amyloliquefaciens contain a large open reading frame between the regions coding for signal sequences and mature protein.. J Bacteriol 159:811–819
    [Google Scholar]
46. Wells J.A., Estell D.A. 1988; Subtilisin - an enzyme designed to be engineered.. Trends Biochem Sci 13:291–297
    [Google Scholar]
47. Wells J.A., Powers D.B. 1986; In vivo formation and stability of engineered disulfide bonds in subtilisin.. J Biol Chem 261:6564–6570
    [Google Scholar]
48. Wells J.A., Ferrari E., Henner D.J., Estell D.A., Chen E.Y. 1983; Cloning, sequencing and secretion of Bacillus amyloliquefaciens subtilisin in Bacillus subtilis. . Nucleic Acids Res 11:7911–7925
    [Google Scholar]