RT Journal Article SR Electronic(1) A1 Serebriiski, I. G. A1 Tsygankov, Y. D.YR 1995 T1 Genetic study of biotin biosynthesis in the obligate methanol-oxidizing bacterium ‘Methylobacillus flagellatum’ JF Microbiology, VO 141 IS 7 SP 1723 OP 1729 DO https://doi.org/10.1099/13500872-141-7-1723 PB Microbiology Society, SN 1465-2080, AB Summary: Twenty-seven mutants defective in the biosynthesis of biotin were generated by N-methyl-N -nitro-N-nitrosoguanidine treatment of the obligate methylotrophic bacterium ‘Methylobacillus flagellatum’. The metabolic lesion in some of these mutants was determined in cross-feeding experiments using known Escherichia coli bio mutants. R-prime plasmids carrying various fragments of the ‘M. flagellatum’ chromosome were used for complementation of bio mutations in ‘M. flagellatum’ and E. coli. By complementation analysis and cross-feeding experiments, ‘M. flagellatum’ Bio- mutants were classified into five groups: bioA, bioB, bioD, bioH, and a group with unidentified bio mutations (bioF or/and bioC). Using R-prime complementation mapping and also Hfr-like mapping, most of the bio genes of ‘M. flagellatum’ identified in this study were localized on the ‘M. flagellatum’ chromosome., UL https://www.microbiologyresearch.org/content/journal/micro/10.1099/13500872-141-7-1723