Summary: Twenty-seven mutants defective in the biosynthesis of biotin were generated by -methyl- -nitro--nitrosoguanidine treatment of the obligate methylotrophic bacterium ‘’. The metabolic lesion in some of these mutants was determined in cross-feeding experiments using known mutants. R-prime plasmids carrying various fragments of the ‘’ chromosome were used for complementation of mutations in ‘’ and . By complementation analysis and cross-feeding experiments, ‘’ Bio mutants were classified into five groups: , and a group with unidentified mutations ( or/and ). Using R-prime complementation mapping and also Hfr-like mapping, most of the genes of ‘’ identified in this study were localized on the ‘’ chromosome.


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