1887

Abstract

Summary: Twenty-three bacteria capable of growing with -phthalate as sole carbon source were isolated from sewage sludge. One of these, named UCC61, was identified as a strain of and was selected for further study. UCC61 was found to carry a single plasmid (named pOPH1) of about 270 kbp in size. The phthalate-utilizing phenotype of UCC61 was unstable and could be lost either by complete curing of pOPH1, or by the deletion of a specific 70 kbp segment from pOPH1. This segment, termed the Pht element, was extensively restriction mapped (using four restriction endonucleases), and was found to be flanked by directly repeated sequences greater than 1-9 kbp in length. Data from comparative restriction analysis of pOPH1 and its deleted derivative were consistent with a deletion mechanism that involves homologous recombination between the direct repeats. UCC61 was found to catabolize -phthalate via 4,5-oxygenation, dehydrogenation and decarboxylation to protocatechuate. These three activities were encoded within the Pht element, with the genes clustered near the right-hand terminus. Protocatechuate metabolism was chromosomally encoded, as also was the ability to catabolize the - and -isomers of phthalate.

Loading

Article metrics loading...

/content/journal/micro/10.1099/13500872-141-7-1673
1995-07-01
2021-05-16
Loading full text...

Full text loading...

/deliver/fulltext/micro/141/7/mic-141-7-1673.html?itemId=/content/journal/micro/10.1099/13500872-141-7-1673&mimeType=html&fmt=ahah

References

  1. Bagdasarian M., Lurz R., Ruckbert B., Franklin F.C.H., Bagdasarian M.M., Frey J., Timmis K.N. 1981; Specific- purpose cloning vectors. II. Broad-host-range high copy number RSFlOlO-derived vectors and a host-vector system for gene cloning in Pseudomonas.. Gene 26:237–247
    [Google Scholar]
  2. Batie C.J., LaHaie E., Ballou D.P. 1987; Purification and characterization of phthalate oxygenase and phthalate oxygenase reductase from Pseudomonas cepacia.. J Biol Chem 262:1510–1518
    [Google Scholar]
  3. Birnboim H., Doly J. 1979; A rapid alkaline extraction procedure for screening recombinant plasmid DNA.. Nucleic Acids Res 7:1513–1523
    [Google Scholar]
  4. Ditta G., Stansfield S., Corbin D., Helinski D.R. 1980; Broad host range DNA cloning system for gram-negative bacteria. Construction of a gene bank of Rhizobium meliloti.. Proc Natl Acad Sci USA 77:7347–7351
    [Google Scholar]
  5. Eaton R.W., Ribbons D.W. 1982a; Utilisation of phthalate esters by micrococci.. Arch Microbiol 132:185–188
    [Google Scholar]
  6. Eaton R.W., Ribbons D.W. 1982b; Metabolism of dibutyl- phthalate and phthalate by Micrococcus sp. strain 12B.. J Bacteriol 151:48–57
    [Google Scholar]
  7. Eaton R.W., Ribbons D.W. 1982c; Biotransformation of 3- methvlphthalate by Micrococcus sp. strain 12B.. f Gen Microbiol 133:2473–2476
    [Google Scholar]
  8. Eaton R.W., Ribbons D.W. 1982d; The transformation of phthalaldehydate by phthalate-grown Micrococcus strain 12B.. Arch Biochem Biophjs 216:288–295
    [Google Scholar]
  9. Eaton R.W., Ribbons D.W. 1982e; Metabolism of dimethyl- phthalate by Micrococcus sp. strain 12B.. J Bacteriol 151:465–467
    [Google Scholar]
  10. Eaton R.W., Timmis K.N. 1986; Spontaneous deletion of a 20- kilobase DNA segment carrying genes specifying isopropylbenzene metabolism in Pseudomonas putida RE204.. J Bacteriol 168:428–430
    [Google Scholar]
  11. Engelhardt G., Wallnofer P.R. 1978; Metabolism of di- andmono-n-butyl phthalate by soil bacteria.. Appl Environ Microbiol 35:243–246
    [Google Scholar]
  12. Evans J.S., Venables W.A. 1990; Degradation of thiophene-2- carboxylate, furan-2-carboxylate, pyrrole-2-carboxylate and other thiophene derivatives by the bacterium Vibrio YC1.. Appl Microbiol Biotechnol 32:715–720
    [Google Scholar]
  13. Franklin F.C.H., Bagdasarian M., Bagdasarian M.M., Timmis K.N. 1981; A molecular and functional analysis of the TOL plasmid pWWO from Pseudomonas putida and cloning of genes for the entire regulated aromatic ring meta-cleavage pathway.. Proc Natl Acad Sci USA 78:7458–7462
    [Google Scholar]
  14. Gaffney T.D., Lessie T.G. 1987; Insertion-sequence-dependent rearrangements of Pseudomonas cepacia plasmid pTGLl.. J Bacteriol 168:224–230
    [Google Scholar]
  15. Grindley N.D.F., Reed R.R. 1985; Transpositional recom-bination in prokaryotes.. Amu Rev Biochem 54:863–896
    [Google Scholar]
  16. Grinter N.J. 1983; A broad host range cloning vector trans¬posable to various replicons.. Gene 21:133–143
    [Google Scholar]
  17. Guerry P., LeBlanc D.J., Falkow S. 1973; General method for the isolation of plasmid deoxyribonucleic acid.. J Bacteriol 116:1064–1066
    [Google Scholar]
  18. Holmes D.S., Quigley M. 1981; A rapid boiling method for the preparation of bacterial plasmids.. Anal Biochem 114:193–197
    [Google Scholar]
  19. Jeenes D.J., Williams P.A. 1982; Excision and integration of degradative pathway genes from TOL plasmid pWWO.. J Bacteriol 150:188–194
    [Google Scholar]
  20. Keyser P., Pujar B.G., Eaton R.W., Ribbons D.W. 1976; Biodegradation of the phthalates and their esters by bacteria.. Environ Health Per sped 18:159–166
    [Google Scholar]
  21. King E.O., Ward M.K., Raney D.E. 1964; Two simple media for the demonstration of pyocyanin and fluorescein.. J Eab Clin Med 43:301–307
    [Google Scholar]
  22. Kurane R., Suzuki T., Takahara Y., Komagata K. 1977a; Identification of phthalate ester-assimilating bacteria.. Agric Biol Chem 41:1031–1038
    [Google Scholar]
  23. Kurane R., Suzuki T., Takahara Y. 1977b; Isolation of microorganisms growing on phthalate esters and degradation of phthalate esters by Pseudomonas acidovorans256-1. Agric Biol Cbem 41:2119–2123
    [Google Scholar]
  24. McClure N.C., Venables W.A. 1987; pTDNl, a catabolic plasmid involved in aromatic amine catabolism in Pseudomonas putida mt-2.. J Gen Microbiol 133:2073–2077
    [Google Scholar]
  25. Meulein P., Downing R.G., Broda P. 1981; Excision of the 40 kb segment of the TOL plasmid from Pseudomonas putida mt-2 involves direct repeats.. Mol & Gen Genet 184:97–101
    [Google Scholar]
  26. Nakazawa T., Hayashi E. 1977; Phthalate metabolism in Pseudomonas testosteroni: accumulation of 4,5-dihydroxyphthalate by a mutant strain.. J Bacterial 131:42–48
    [Google Scholar]
  27. Nakazawa T., Hayashi E. 1978; Phthalate and 4-hydrox- yphthalate metabolism in Pseudomonas testosteroni-. purification and properties of 4,5-dihydroxyphthalate decarboxylase.. Appl Environ Microbiol 36:264–269
    [Google Scholar]
  28. Nomura Y., Harashima S., Oshima Y. 1989a; A simple method for detection of enzyme activities involved in the initial step of phthalate degradation in microorganisms.. J Ferment Bioeng 67:291–296
    [Google Scholar]
  29. Nomura Y., Takada N., Oshima Y. 1989b; Isolation and identification of phthalate-utilizing bacteria.. J Ferment Bioeng 67:297–299
    [Google Scholar]
  30. Nomura Y., Harashima S., Oshima Y. 1990a; PHT, a transmissible plasmid responsible for phthalate utilization in Pseudomonas putida.. J Ferment Bioeng 70:295–300
    [Google Scholar]
  31. Nomura Y., Harashima S., Takada N., Oshima Y. 1990b; Phthalate degradation in Pseudomonas putida.. Ann NY Acad Sci 13:776–780
    [Google Scholar]
  32. Ono K., Nozaki M., Hayashi O. 1970; Purification and some properties of protocatechuate 4,5-dioxygenase.. Biochim Biophys Acta 220:224–228
    [Google Scholar]
  33. Pujar B.G., Ribbons D.W. 1985; Phthalate metabolism in Pseudomonas fluorescens PHK: purification and properties of 4,5- dihydroxyphthalate decarboxylase.. Appl Environ Microbiol 49:374–376
    [Google Scholar]
  34. Ribbons D.W., Keyser P., Kunz D.A., Taylor B.F., Eaton R.W., Anderson B.N. 1984; Microbial degradation of phthalates.. In Microbial Degradation of Organic Compounds pp. 371–397 Gibson D.T. Edited by New York: Dekker;
    [Google Scholar]
  35. Saint C.P., Ribbons D.W. 1990; A catabolic plasmid involved in 4-methyl-o-phthalate and 4-hydroxy-Ao-phthalate degradation in Pseudomonas cepacia.. FEMS Microbiol Lett 69:323–328
    [Google Scholar]
  36. Saint C.P., Venables W.A. 1990; Loss of Tdn catabolic genes by deletion and curing of plasmid pTDNl in Pseudomonas putida: rate and mode of loss are substrate and pH dependent.. J Gen Microbiol 136:627–636
    [Google Scholar]
  37. Saint C.P., McClure N.C., Venables W.A. 1990; Physical map of the aromatic amine and w-toluate catabolic plasmid pTDNl in Pseudomonas putida-. location of a unique meta-cleavage pathway.. J Gen Microbiol 136:615–625
    [Google Scholar]
  38. Sambrook J., Fritsch E.F., Maniatis T. 1989 Molecular Cloning-, a Laboratory Manual. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory;
    [Google Scholar]
  39. Sayler G.S., Hooper S.W., Layton A.C., Henry-King J.M. 1990; Catabolic plasmids of environmental and ecological sign-ificance.. Microb Ecol 19:1–20
    [Google Scholar]
  40. Stanier R.Y., Palleroni N.J., Doudoroff M. 1966; The aerobic pseudomonads, a taxonomic study.. J Gen Microbiol 43:159–271
    [Google Scholar]
  41. Staskawicz B., Dahlbeck D., Keen N., Napoli C. 1987; Molecular characterization of cloned avirulence genes from race O and race 1 of Pseudomonas syringae pv.glycinea.. J Bacterial 169:5789–5794
    [Google Scholar]
  42. Tsuda M., lino T. 1988; Identification and characterisation of Tn4653, a transposon covering the toluene transposon Tn4651 on TOL plasmid pWWO.. Mol & Gen Genet 213:72–77
    [Google Scholar]
  43. Wheatcroft R., Williams P.A. 1981; Rapid methods for the study of both stable and unstable plasmids in Pseudomonas.. J Gen Microbiol 124:433–437
    [Google Scholar]
  44. Wyndham R.C., Straus N.A. 1988; Chlorobenzoate catabolism and interactions between Alcaligenes and Pseudomonas species from Bloody Run Creek.. Arch Microbiol 150:230–236
    [Google Scholar]
  45. Wyndham R.C., Singh R.K., Straus N.A. 1988; Catabolic instability, plasmid gene deletion and recombination in Alcaligenes sp. BR 60.. Arch Microbiol 150:237–243
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/13500872-141-7-1673
Loading
/content/journal/micro/10.1099/13500872-141-7-1673
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error