1887

Abstract

Summary: Enterotoxigenic are known to secrete several types of toxins including STb, a heat-stable enterotoxin. STb enterotoxin production was studied in wild-type strains. Using a quantitative STb-specific inhibition ELISA, the amount of toxin present in the culture supernatant fractions of various strains was determined. Variation in the production of STb toxin was observed for the wild-type strains. For strain 82-4247 grown in trypticase soy broth, the toxin was produced after 4 h of growth and was maximal after about 57 h of growth. The amount of toxin in the culture supernatant fraction increased concomitantly with bacterial growth. Using the rat loop assay, the biological activity of STb was retained even after the logarithmic phase of growth when STb production levelled off (i.e. from 24 to 74 h). STb production by strain 82-4247 varied with the culture medium used. In particular, addition of 1-0% (w/v) compared to 0-1 % glucose to Davis minimal medium decreased STb production, whereas addition of 1-0% (w/v) glycerol did not affect STb production. Addition of exogenous cAMP reversed the repressive effect of glucose. Using mutant strains, STb production was shown to be subject to catabolite repression.

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1995-07-01
2021-10-26
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References

  1. Adhya S., Garges S. 1982; How cyclic AMP and its receptor protein act inEscherichia coli. . Cell 29:287–289
    [Google Scholar]
  2. Alderette J.F., Robertson D.C. 1977a; Repression of heat- stable enterotoxin synthesis in enterotoxigenicEscherichia coli. . Infect Immun 17:629–633
    [Google Scholar]
  3. Alderette J.F., Robertson D.C. 1977b; Nutrition and enterotoxin synthesis by enterotoxigenic strains ofEscherichia coli: defined medium for production of heat-stable enterotoxin.. Infect Immun 15:781–788
    [Google Scholar]
  4. Al-Jumaili I., Burke D.A., Scotland S.M., Al-Mardini H., Record C.O. 1992; A method of enhancing verotoxin production byEscherichia coli. . FEMS Microbiol Lett 93:121–126
    [Google Scholar]
  5. Amirmozafari N., Robertson D.C. 1993; Nutritional require-ments for synthesis of heat-stable enterotoxin byYersinia enterocolitica. . Appl Environ Microbiol 59:3314–3320
    [Google Scholar]
  6. Bossé M., Handl C.E., Lortie L-A., Harel J., Dubreuil J.D. 1993; Fusion of the genes encodingEscherichia coli heat-stable enterotoxin b (STb) and the maltose-binding protein to obtain mature STb enterotoxin.. J Gen Microbiol 139:631–638
    [Google Scholar]
  7. Boyer H.V., Roulland-Dussoix D. 1969; A complementation analysis of the restriction and modification of DNA inEscherichia coli. . J Mol Biol 41:459–472
    [Google Scholar]
  8. Burgess M.N., Bywater R.J., Cowley C.M., Mullan N.A., Newsome P.M. 1978; Biological evaluation of a methanol- solubleEscherichia coli enterotoxin in infant mice, pigs, rabbits and calves.. Infect Immun 59:198–203
    [Google Scholar]
  9. Dubreuil J.D., Fairbrother J.M., Lallier R., Lariviére S. 1991; Production and purification of heat-stable enterotoxin b from a porcineEscherichia coli strain.. Infect Immun 58:198–203
    [Google Scholar]
  10. Dreyfus L.A., Urban R.G., Whipp S.C., Slaughter C., Tachias K., Kupersztoch Y.M. 1992; Purification of the STb enterotoxin ofEscherichia coli and the role of selected amino acids on its secretion, stability and toxicity.. Mol Microbiol 6:2397–2406
    [Google Scholar]
  11. Echeverria P., Seriwatana J., Patamaroj U., Moseley S.L, Chityothin O., Chaicumpa W. 1984; Prevalence of heat-stable II enterotoxigenicEscherichia coli in pigs, water, and people at farms in Thailand as determined by DNA hybridization.. J Clin Microbiol 19:489–491
    [Google Scholar]
  12. Evans D.G., Evans D.I., Karjalainen T.K., Lee C.-H. 1991; Production of colonization factor antigen II of enterotoxigenicEscherichia coli is subject to catabolite repression.. Curr Microbiol 23:71–74
    [Google Scholar]
  13. Fujii Y., Hayashi M., Hitotsubashi S., Fuke Y., Yamanaka H., Okamoto K. 1991; Purification and characterization ofEscherichia coli heat-stable enterotoxin II.. J Bacteriol 173:5516–5522
    [Google Scholar]
  14. Garges S. 1994; Activation of transcription inEscherichia coli, the cyclic AMP receptor protein.. In Transcription: Mechanisms and Regulation. Series on Molecular and Cellular Biology 3: pp. 343–352 Conaway R. C., Conaway. J. W. Edited by New York;: Raven Press.;
    [Google Scholar]
  15. Goransson M., Forsman K., Nilsson P., Uhlin B.E. 1989; Upstream activating sequences that are shared by two differently transcribed operons mediated cAMP-CRP regulation of pilus- adhesin inEscherichia coli. . Mol Microbiol 3:1557–1565
    [Google Scholar]
  16. Guerrant R.L., Hugues J.M., Lima N.L., Crane J. 1990; Diarrhea in developed and developing countries: magnitude, special settings and etiologies.. Rev Infect Dis Suppl 12:S41–S50
    [Google Scholar]
  17. Gyles C, So M., Falkow S. 1974; The enterotoxin plasmids ofEscherichia coli. . J Infect Dis 130:40–49
    [Google Scholar]
  18. Handl C.E., Flock J.-I. 1990; Studies on heat-stable enterotoxin type II (STII or STb) fromEscherichia coli. . In Molecular Pathogenesis of Gastrointestinal Infections, pp. 307–311 Wadstrom T., Makela P. H., Svennerholm A.-M., Wolf-Watz. H. Edited by New York:: Plenum;
    [Google Scholar]
  19. Handl C.E., Flock J.-l. 1992; STb producingEscherichia coli are rarely associated with human infantile diarrhoea.. J Diarrhoeal Dis Res 10:37–38
    [Google Scholar]
  20. Handl C.E., RÖnnberg B., Nilsson B., Olsson E., Johnsson H., Flock J.-I. 1988; Enzyme-linked immunosorbent assay forEscherichia coli heat-stable enterotoxin type II.. J Clin Microbiol 26:1555–1560
    [Google Scholar]
  21. Handl C.E., Harel J., Flock J.-l., Dubreuil J.D. 1993; High yield of active STb enterotoxin from a fusion protein (MBP-STb) expressed inEscherichia coli. . Prot Expr Purif 4275–281
    [Google Scholar]
  22. Hawkes R. 1982; Identification of concavalin A-binding proteinsafter sodium dodecyl sulfate-gel electrophoresis and protein blotting.. Anal Biochem 123:143–146
    [Google Scholar]
  23. Hedegaard L., Danchin A. 1985; Thecya gene region ofErwinia chrjsanthemi 13374: organization and gene products.. Mol & Gen Genet 201:38–42
    [Google Scholar]
  24. Hitotsubashi S., Fujii Y., Yamanaka H., Okamoto K. 1992; Some properties of purifiedEscherichia coli heat-stable enterotoxin II.. Infect Immun 60:4468–4474
    [Google Scholar]
  25. Kolb A., Busby S., Buc H., Garges S., Adhya S. 1993; Transcription regulation by cAMP and its receptor protein.. Annu Rev Biochem 62:749–795
    [Google Scholar]
  26. Kupersztoch Y.M., Tachias K., Moomaw C.R., Dreyfus L.A., Urban R., Slaughter C., Whipp S. 1990; Secretion of methanol- insoluble heat-stable enterotoxin (STb): energy- and secA-de- pendent conversion of pre-STb to an intermediate indistinguishable from the extracellular toxin.. J Bacteriol 172:2427–2432
    [Google Scholar]
  27. Lee C.H., Moseley S.L, Whipp S.C., Gyles C.L., So M. 1983; Characterization of the gene encoding heat-stable toxin II and preliminary molecular epidemiological studies of enteropathogenicEscherichia coli heat-stable toxin II producers.. Infect Immun 42:264–268
    [Google Scholar]
  28. Levner M., Wiener F.P., Rubin B.A. 1977; Induction ofEscherichia coli andVibrio cholerae enterotoxins by an inhibitor of protein synthesis.. Infect Immun 15:132–137
    [Google Scholar]
  29. Lortie L.-A., Dubreuil J.D., Harel J. 1991a; Characterization ofEscherichia coli strains producing heat-stable enterotoxin b (STb) isolated from humans with diarrhea.. J Clin Microbiol 29:656–659
    [Google Scholar]
  30. Lortie L.-A., Harel J., Fairbrother J.M., Dubreuil J.D. 1991b; Evaluation of three new techniques for the detection of STb- positiveEscherichia coli strains.. Mol Cell Probes 5:271–275
    [Google Scholar]
  31. Lortie L.-A., Harel J., Fairbrother J.M., Dubreuil J.D. 1991c; Immunodot detection ofEscherichia coli heat-stable enterotoxin b by using enhanced chemiluminescence reaction.. J Clin Microbiol 29:2250–2252
    [Google Scholar]
  32. Maniatis T., Fritsch E.F., Sambrook J. 1982 In Molecular Cloning-, a Eaboratory Manual. Cold Spring Harbor, NY;: Cold Spring Harbor Laboratory.;
    [Google Scholar]
  33. Mainil J.G., Moseley S.L., Schneider R.A., Sutch K., Casey T.A., Moon H.W. 1986; Hybridization of bovineEscherichia coli isolates with gene probes for four enterotoxins (STaP, STaH, STb, LT).. Am J Vet Res 47:1145–1148
    [Google Scholar]
  34. Moon H.W., Schneider R.A., Moseley S.L. 1986; Comparative prevalence of four enterotoxin genes amongEscherichia coli isolated from swine.. Am J Vet Res 47:210–212
    [Google Scholar]
  35. Picken R.N., Mazaitis A.J., Maas W.K., Rey M., Heyneker H. 1983; Nucleotide sequence of the gene for heat-stable enterotoxin II ofEscherichia coli. . Infect Immun 42:269–275
    [Google Scholar]
  36. Reeves P. 1972; Molecular biology.. In Molecular Biology, Biochemistry and Biophysics, 11: pp. 35–45 Kleinzeller A., Springer G. F., Wittmann. H. G. Edited by London:: Chapman & Hall.;
    [Google Scholar]
  37. Söderlind O., Thafvelin B., MÖilby R. 1988; Virulence factors inEscherichia coli strains isolated from Swedish piglets with diarrhea.. J Clin Microbiol 26:879–884
    [Google Scholar]
  38. Spandau D.F., Lee C.H. 1987; Determination of the promoter strength of the gene encodingEscherichia coli heat-stable enterotoxin II.. J Bacteriol 169:1740–1744
    [Google Scholar]
  39. Urban R.G., Pipper E.M., Dreyfus L.A., Whipp S.C. 1990; High-level production ofEscherichia coli STb enterotoxin and quantification by a direct enzyme-linked immunosorbent assay.. J Clin Microbiol 28:2383–2388
    [Google Scholar]
  40. Wang Y.-P., Giblin L, O'Gara F. 1993; TheEscherichia coli cAMP receptor protein (CRP) represses theRhizobium meliloti dctA promoter in a cAMP-dependent fashion.. Mol Microbiol 8:253–259
    [Google Scholar]
  41. Weikel C.S., Nellans H.N., Guerran R.L. 1986; In vivo andin vitro effects of a novel enterotoxin, STb, produced byEscherichia coli. . J Infect Dis 153:893–901
    [Google Scholar]
  42. Whipp S.C. 1987; Protease degradation ofEscherichia coli heat- stable, mouse-negative, pig-positive enterotoxin.. Infect Immm 55:2057–2060
    [Google Scholar]
  43. Whipp S.C. 1991; Intestinal responses to enterotoxigenicEscherichia coli heat-stable toxin b in non-porcine species.. Am J Vet Res 52:734–737
    [Google Scholar]
  44. Whipp S.C, Moseley S.L, Moon H.W. 1986; Microscopic alterations in jejunal epithelium of 3-week-old pigs induced by pig- specific, mouse-negative, heat-stableEscherichia coli enterotoxin.. Am J Vet Res 47:615–618
    [Google Scholar]
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