Coordination of sucrose uptake and respiration in the yeast Free

Abstract

Summary: Screening in batch cultures identified as a yeast that exhibits the Kluyver effect for sucrose: this disaccharide can be respired but, even under oxygen-limited conditions, alcoholic fermentation of sucrose does not occur. Ethanol, glycerol and arabitol were the main fermentation products during oxygen-limited growth on glucose in chemostat cultures. None of these fermentation products were produced in oxygen-limited chemostat cultures grown on sucrose and the fraction of the sucrose that could not be respired remained unused in the culture medium. This absence of alcoholic fermentation was not due to repression of the key fermentative enzymes pyruvate decarboxylase and alcohol dehydrogenase. In contrast to some other yeasts that exhibit a Kluyver effect, did not exhibit a preference for ethanol in batch cultures grown on mixtures of ethanol and sucrose. Sucrose metabolism in involves intracellular hydrolysis by an α-glucosidase. Incubation of weakly buffered cell suspensions with sucrose led to a rapid transient alkalinization, indicating the presence of a sucrose-proton symport system. The apparent substrate saturation constant of the sucrose-uptake system was 0.2 mmol l. Sucrose-dependent alkalinization rates were much lower in samples from oxygen-limited cultures than in samples from aerobic cultures. Transient responses of to oxygen limitation were investigated by applying a sudden decrease in the oxygen feed to aerobic sugar-limited chemostat cultures. In glucose-grown cultures, this led to alcoholic fermentation and no significant accumulation of sugar occurred after the switch. In sucrose-limited cultures, sugar accumulation occurred instantaneously after the switch, and ethanol formation was virtually absent. The results indicate that the Kluyver effect for sucrose in , i.e. the adjustment of the glycolytic flux to the cells' respiratory capacity, is effected by rapid down-regulation of the capacity of the sucrose carrier under oxygen-limited conditions.

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1995-07-01
2024-03-28
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References

  1. Barnett J.A. 1981; The utilization of disaccharides and some other sugars by yeasts.. Adv Carbohydr Chem Biochem 39:347–404
    [Google Scholar]
  2. Barnett J.A. 1992; Some controls on oligosaccharide utilization by yeasts: the physiological basis of the Kluyver effect.. FEMS Microbiol Lett 100:371–378
    [Google Scholar]
  3. Barnett J.A., Sims A.P. 1982; The requirement of oxygen for the active transport of sugars into yeasts.. J Gen Microbiol 128:2303–2312
    [Google Scholar]
  4. Barnett J.A., Payne R.W., Yarrow D. 1990 Yeasts: Characteristics and Identification, 2nd. Cambridge:: Cambridge University Press.;
    [Google Scholar]
  5. Bush D.R. 1993; Proton-coupled sugar and amino acid transporters in plants.. Anna Ren Plant Physiol Plant Mol Biol 44:513–542
    [Google Scholar]
  6. Carvalho-Silva M., Spencer-Martins I. 1990; Modes of lactose uptake in the yeast species Kluyveromyces marxianus. . Antonie Leeuwenhoek 57:77–81
    [Google Scholar]
  7. Dickson R.C., Barr K. 1983; Characterization of lactose transport in Kluyveromyces lactis. . J Bacteriol 154:1245–1251
    [Google Scholar]
  8. van Dijken J.P., van Den Bosch E., Hermans J.J., Rodrigues de Miranda L., Scheffers W.A. 1986; Alcoholic fermentation by ‘non-fermentative’ yeasts.. Yeast 2:123–127
    [Google Scholar]
  9. Holzer H. 1976; Catabolite inactivation in yeast.. Trends Biochem Sci 1:178–181
    [Google Scholar]
  10. Johansson M., Sjöström J.E. 1984; Enhanced production of glycerol in an alcohol dehydrogenase (ADH1) deficient mutant of Saccharomyces cerevisiae. . Biotechnol Lett 6:49–54
    [Google Scholar]
  11. Kaliterna J., Weusthuis R.A., Castrillo J.I., van Dijken J.P., Pronk J.T. 1995; Transient responses of Candida utilis to oxygen limitation:regulation of the Kluyver effect for maltose.. Yeast 11: (in press).
    [Google Scholar]
  12. Noorman H.J., Baksteen J., Heijnen J.J., Luyben K.Ch.A.M. 1991; The bioreactor overflow device: an undesired selective separator in continuous cultures?. J Gen Microbiol 137:2171–2177
    [Google Scholar]
  13. Postma E., Verduyn C., Scheffers W.A., van Dijken J.P. 1989; Enzymic analysis of the Crabtree effect in glucose-limited chemostat cultures of Saccharomyces cerevisiae. . Appl Environ Microbiol 55:159–165
    [Google Scholar]
  14. Santos E., Rodriguez L., Elorza M.V., Sentandreu R. 1982; Uptake of sucrose by Saccharomyces cerevisiae. . Arch Biochem Biophys 216:652–660
    [Google Scholar]
  15. Schulz B., Höfer M. 1986; Utilization of lactose in nonrespiring cells of the yeast Debaryomyces polymorpbus. . Arch Microbiol 145:367–371
    [Google Scholar]
  16. Serrano R. 1977; Energy requirements for maltose transport in yeast.. Eur J Biochem 80:97–102
    [Google Scholar]
  17. Sims A.P., Barnett J.A. 1978; The requirement of oxygen for the utilization of maltose, cellobiose and D-galactose by certain anaerobically fermenting yeasts (Kluyver effect).. J Gen Microbiol 106:277–288
    [Google Scholar]
  18. Sims A.P., Barnett J.A. 1991; Levels of activity of enzymes involved in anaerobic utilization of sugars by six yeast species: observations towards understanding the Kluyver effect.. FEMS Microbiol Lett 77:295–298
    [Google Scholar]
  19. Sims A.P., Ståhlbrand H., Barnett J.A. 1991; The role of pyruvate decarboxylase in the Kluyver effect in the food yeast, Candida utilis. . Yeast 7:479–487
    [Google Scholar]
  20. van Urk H., Schipper D., Breedveld G.J., Mak P.R., Scheffers W.A., van Dijken J.P. 1989; Localization and kinetics of pyruvate metabolizing enzymes in Saccharomyces cerevisiae CBS 8066 and Candida utilis CBS 621.. Biochim Biophys Acta 992:78–86
    [Google Scholar]
  21. Van Leeuwen C.C.M., Weusthuis R.A., Postma E., van den Broek P.J.A., van Dijken J.P. 1992; Maltose/proton cotransport in Saccharomyces cerevisiae. Comparative study with cells and plasma membrane vesicles.. Biochem J 284:441–445
    [Google Scholar]
  22. Weusthuis R.A., Adams H., Scheffers W.A., van Dijken J.P. 1993; Energetics and kinetics of maltose transport in Saccharomyces cerevisiae: a continuous culture study.. Appl Environ Microbiol 59:3102–3109
    [Google Scholar]
  23. Weusthuis R.A., Visser W., Pronk J.T., Scheffers W.A., van Dijken J.P. 1994a; Effects of oxygen limitation on sugar metabolism in yeasts: a continuous-culture study of the Kluyver effect.. Microbiology 140:703–715
    [Google Scholar]
  24. Weusthuis R.A., Luttik M.A.H., Scheffers W.A., van Dijken J.P., Pronk J.T. 1994b; Is the Kluyver effect caused by product inhibition?. Microbiology 140:1723–1729
    [Google Scholar]
  25. Williamson P.R., Huber M.A., Bennett J.E. 1993; Role of maltase in the utilization of sucrose by Candida albicans. . Biochem J 291:765–771
    [Google Scholar]
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