1887

Abstract

Summary: The monoclonal antibody (mAb) B9E, which reacts with a cell wall surface determinant of serotype A, and a polyclonal monospecific antiserum against the antigen 6 (IF6) were used to investigate the expression of the antigens responsible for the serotype specificity in under different growth conditions. By indirect immunofluorescence, both antibodies reacted with the cell wall surface of serotype A yeast cells and germ tubes grown but no reactivity was observed with serotype B yeast cells. In some cases, only a weak reactivity restricted to a zone close to the parent yeast cell was observed in serotype B germ tubes stained with mAb B9E. Both antibodies reacted strongly with yeast cells and germ tubes present in kidney abscesses from rabbits infected with both serotypes, but only serotype A yeast cells and germ tubes present in smears from patients with vulvovaginal candidiasis reacted with B9E and IF6 antibodies. The expression of antigens reactive with both antibodies was modulated by the pH of the environment in which the fungus was grown. Both antibodies showed a similar pattern of reactivity when studied with a spectrofluorometer. Serotype A yeast cells showed maximum reactivity when cells were grown on Sabouraud dextrose broth supplemented with yeast extract at pH 4.6. The lowest reactivity was observed in cells grown at pH 2.0. Conversely, the reactivity of serotype B yeast cells increased at alkaline pH values, the highest being in cells grown at pH values of 7.2 and 9.5. A precise use of the methods employed in studies on serotype prevalence will be important to avoid the influence of pH on the expression of antigens conferring serotype specificity.

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1995-07-01
2021-08-05
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References

  1. Brawner D.L. 1991; Comparison between methods for serotyping of Candida albicans produces discrepancies in results.. J Clin Microbiol 29:1020–1025
    [Google Scholar]
  2. Brawner D.L., Cutler J.E. 1984; Variability in expression of cell surface determinant on Candida albicans as evidenced by an agglutinating monoclonal antibody.. Infect Immun 43:966–972
    [Google Scholar]
  3. Brawner D.L., Cutler J.E. 1989; Oral Candida albicans isolates from nonhospitalized normal carriers, immunocompetent hospitalized patients, and immunocompromised patients with or without acquired immunodeficiency syndrome.. J Clin Microbiol 27:1335–1341
    [Google Scholar]
  4. Brawner D.L., Anderson G.L., Yuen K.Y. 1992; Serotype prevalence of Candida albicans from blood culture isolates.. J Clin Microbiol 30:149–153
    [Google Scholar]
  5. Drohuet E., Mercier-Soucy L., Montplaisir S. 1975; Sensibilité et résistance des levures pathogenes aux 5-fluoropyrimidines. I. Relation entre les phénotypes de résistance a la 5-fluorocytosine, le serotype de Candida albicans et l’écologie de différentes espèces de Candida d’origine humaine.. Ann Microbiol (Paris) 126B:25–39
    [Google Scholar]
  6. Guinet R.M.F., Gabriel S.M. 1980; Candida albicans group A-specific soluble antigens demonstrated by quantitative immuno-electrophoresis.. Infect Immun 29:853–858
    [Google Scholar]
  7. Hasendever H.F. 1965; The antigens of Candida albicans.. Am Rep Respir Dis 92:150–158
    [Google Scholar]
  8. Hasendever H.F., Mitchell W.O. 1961; Antigenic studies of Candida. I. Observations of two antigenic groups in Candida albicans.. J Bacteriol 82:570–573
    [Google Scholar]
  9. Hasendever H.F., Mitchell W.O., Loewe J. 1961; Antigenic studies of Candida. II. Antigenic relation of Candida albicans group A and group B to C. stellatoidea and C. tropicalis.. J Bacteriol 82:574–577
    [Google Scholar]
  10. Kennedy M.J., Johnson A.M., Volz P.A., Neely A.N., Yancey R.J. 1992; Virulence and adhesive properties of serotypes A and B of Candida albicans isolated from paediatric burn patients.. J Med Microbiol 36:428–436
    [Google Scholar]
  11. Kobayashi H., Giummelly P., Takahashi S., Ishida M., Sato J., Takaku M., Nishidate Y., Shibata N., Okawa Y., Suzuki S. 1991; Candida albicans serotype A strains grow in yeast extract-added Sabouraud liquid medium at pH 2·0, elaborating mannans without β-l,2 linkage and phosphate group.. Biochem Biophjs Res Commun 175:1003–1009
    [Google Scholar]
  12. Kobayashi H., Shibata N., Suzuki S. 1992; Evidence for oligomannosyl residues containing both β-l,2 and α-1,2 linkages as a serotype A-specific epitope(s) in mannans of Candida albicans.. Infect Immun 60:2106–2109
    [Google Scholar]
  13. Kobayashi H., Takahashi S., Shibata N., Miyauchi M., Ishida M., Sato J., Maeda K., Suzuki S. 1994; Structural modification of cell wall mannans of Candida albicans serotype A strains grown in yeast extract-Sabouraud liquid medium under acidic conditions.. Infect Immun 62:968–973
    [Google Scholar]
  14. Laemmli U.K. 1970; Cleavage of structural proteins during the assembly of the head of bacteriophage T4.. Nature 227:680–685
    [Google Scholar]
  15. Miyakawa Y., Kagaya K., Fukazawa Y., Soe G. 1986; Production and characterization of agglutinating monoclonal antibodies against predominant antigenic factors for Candida albicans.. J Clin Microbiol 23:881–886
    [Google Scholar]
  16. Miyakawa Y., Kuribayashi T., Kagaya K., Suzuki M., Nakase T., Fukazawa Y. 1992; Role of specific determinants in mannan of Candida albicans serotype A in adherence to human buccal epithelial cells.. Infect Immun 60:2493–2499
    [Google Scholar]
  17. Neely A.N., Odds F.C., Basatia B.K., Holder I.A. 1988; Characterization of Candida isolates from pediatric burn patients.. J Clin Microbiol 26:1645–1649
    [Google Scholar]
  18. Pontón J., Jones J.M. 1986; Analysis of cell wall extracts of Candida albicans by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and western blot techniques.. Infect Immun 53:565–572
    [Google Scholar]
  19. Pontón J., Marot-Leblond A., Ezkurra P.A., Barturen B., Robert R., Senet J.M. 1993; Characterization of Candida albicans cell wall antigens with monoclonal antibodies.. Infect Immun 61:4842–4847
    [Google Scholar]
  20. Poulain D., Tronchin G., Vernes A., Popeye R., Biguet J. 1983; Antigenic variations of Candida albicans in vivo and in vitro. Relationships between P antigens and serotypes.. Sabouraudia 21:99–112
    [Google Scholar]
  21. Poulain D., Hopwood V., Vernes A. 1985; Antigenic variability of Candida albicans.. Crit Rev Microbiol 12:223–270
    [Google Scholar]
  22. Quindós G., Pontón J., Cisterna R. 1987; Detection of antibodies to Candida albicans germ tube in the diagnosis of systemic candidiasis.. Eur J Clin Microbiol 6:142–145
    [Google Scholar]
  23. Reiss E., De Repentigny L., Kuykendall R.J., Carter A.W., Galindo R., Auger P., Bragg S.L., Kaufman L. 1986; Monoclonal antibodies against Candida tropicalis mannan: antigen detection by enzyme immunoassay and immunofluorescence.. J Clin Microbiol 24:796–802
    [Google Scholar]
  24. Smail E. H., Jones J.M. 1984; Demonstration and solubilization of antigens expressed primarily on the surfaces of Candida albicans germ tubes.. Infect Immun 45:74–81
    [Google Scholar]
  25. Summers D.F., Grollman A., Hasendever H.F. 1964; Polysaccharide antigens of Candida cell wall.. J Immunol 92:491–499
    [Google Scholar]
  26. Sundstrom P.M., Kenny G.E. 1984; Characterization of antigens specific to the surface of germ tubes of Candida albicans by immunofluorescence.. Infect Immun 43:850–855
    [Google Scholar]
  27. Towbin H., Staehelin T., Gordon J. 1979; Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications.. Proc Natl Acad Sci USA 764350–4354
    [Google Scholar]
  28. Tsuchiya T., Takaguchi M., Fukazawa Y., Shinoda T. 1984; Serological characterization of yeasts as an aid in identification and classification.. In Methods in Microbiology 16 pp. 75–126 Edited by Bergan T. London: Academic Press;
    [Google Scholar]
  29. Voller A., Bidwell D., Bartlett A. 1980; Enzyme-linked immunosorbent assay.. In Manual of Clinical Immunology pp. 359–371 Edited by Rose N. R., Friedman H. Washington, DC: American Society for Microbiology;
    [Google Scholar]
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