1887

Abstract

A facultatively chemolithotrophic thiocyanate-degrading bacterium, strain THI 011, which was previously isolated from activated sludge and tentatively named sp., was studied taxonomically and phylogenetically. This bacterium utilizes thiocyanate as sole energy source and the specific growth rate for chemolithoautotrophic growth with thiocyanate was 0059 h. Molecular phylogenetic relationships of strain THI 011 to and members of the genus were elucidated by comparing 16S rRNA gene sequences. Binary sequence comparisons showed that strain THI 011 was most related to , at a similarity level of 970%, and was most similar to , at a level of 991%. A neighbour-joining phylogenetic tree showed that strain THI 011 formed a cluster together with and known species of the genus within the α-3 subclass of the DNA-DNA hybridization assays and phenotypic studies indicated that strain THI 011 differed from and known species of the genus On the basis of these results, we propose to classify strain THI 011 into a new species of the genus with the name sp. nov. We also propose to transfer to the genus and present an emended description of the genus.

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1995-06-01
2021-05-08
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